Construct: ORF TRCN0000471609
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012750.1_s317c1
- Derived from:
- ccsbBroadEn_04546
- DNA Barcode:
- CCAATCCGCCACAACTATGGTCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF185 (91445)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471609
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91445 | RNF185 | ring finger protein 185 | NM_152267.4 | 100% | 100% | |
| 2 | human | 91445 | RNF185 | ring finger protein 185 | NM_001135825.2 | 70.8% | 70.8% | 194_195ins168 |
| 3 | human | 112268469 | LOC112268469 | uncharacterized LOC112268469 | XR_002959800.1 | 19.3% | (many diffs) | |
| 4 | human | 91445 | RNF185 | ring finger protein 185 | NR_024210.2 | 17.1% | 1_170del;533_699del;914_3349del | |
| 5 | human | 91445 | RNF185 | ring finger protein 185 | NR_024212.2 | 15.6% | (many diffs) | |
| 6 | human | 91445 | RNF185 | ring finger protein 185 | NR_024211.2 | 14.5% | 1_170del;362_363ins113;634_3069del | |
| 7 | human | 91445 | RNF185 | ring finger protein 185 | NR_024209.2 | 14.1% | 1_268del;460_461ins113;732_3167del | |
| 8 | mouse | 193670 | Rnf185 | ring finger protein 185 | NM_001290472.1 | 93% | 98.4% | (many diffs) |
| 9 | mouse | 193670 | Rnf185 | ring finger protein 185 | NM_145355.5 | 78.3% | 82.8% | (many diffs) |
| 10 | mouse | 193670 | Rnf185 | ring finger protein 185 | NM_001290473.1 | 70.8% | 66.3% | (many diffs) |
| 11 | mouse | 193670 | Rnf185 | ring finger protein 185 | XM_006514594.3 | 70.8% | 66.3% | (many diffs) |
| 12 | mouse | 193670 | Rnf185 | ring finger protein 185 | NR_110959.1 | 15.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 642
- ORF length:
- 576
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aagcaagggg ccctcggcct ctgcatctcc tgagaactcc agtgcagggg 121 ggcccagtgg gagcagcaat ggcgctggcg agagcggagg gcaggacagc actttcgagt 181 gcaacatctg cttggacaca gccaaggatg ccgtcatcag cctgtgtggc cacctcttct 241 gttggccgtg tttacatcag tggttggaga ccagaccTAA CAGACAGGTG TGTCCTGTTT 301 GCAAAGCTGG CATCAGCCGA GACAAGGTCA TCCCCCTCTA TGGAAGGGGC AGCACTGGGC 361 AACAGGACCC CAGAGAGAAG ACCCCTCCTC GTCCTCAAGG ACAGAGGCCA GAGCCGGAGA 421 ATAGAGGGGG ATTTCAAGGA TTTGGATTTG GAGATGGTGG CTTCCAGATG TCTTTTGGAA 481 TTGGGGCATT TCCCTTTGGG ATATTTGCCA CAGCATTTAA TATAAATGAT GGGCGGCCTC 541 CTCCAGCTGT CCCTGGGACA CCCCAGTATG TGGACGAGCA GTTCCTGTCA CGCCTCTTCC 601 TATTTGTGGC CCTGGTGATC ATGTTCTGGC TCCTGATTGC CTACCCAACT TTCTTGTACA 661 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 721 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 781 AGGACGACCA ATCCGCCACA ACTATGGTCA AACGCGTTAA GTCgacaatc aacctctgga 841 ttacaaaatt tgtgaaagat t