Construct: ORF TRCN0000471625
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004334.1_s317c1
- Derived from:
- ccsbBroadEn_16135
- DNA Barcode:
- CGAGCCCGAAAGTTCCTGTAATAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNX32 (254122)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471625
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 254122 | SNX32 | sorting nexin 32 | NM_152760.3 | 99.9% | 100% | 24G>C |
| 2 | human | 254122 | SNX32 | sorting nexin 32 | XM_006718488.4 | 97.1% | 94.8% | (many diffs) |
| 3 | human | 254122 | SNX32 | sorting nexin 32 | XM_024448417.1 | 87.6% | 73.4% | (many diffs) |
| 4 | human | 254122 | SNX32 | sorting nexin 32 | XM_024448418.1 | 86.4% | 72.2% | (many diffs) |
| 5 | human | 254122 | SNX32 | sorting nexin 32 | XM_017017473.1 | 68% | 66.1% | (many diffs) |
| 6 | human | 254122 | SNX32 | sorting nexin 32 | XR_001747821.2 | 65.5% | (many diffs) | |
| 7 | human | 254122 | SNX32 | sorting nexin 32 | XM_024448419.1 | 61.6% | 49.3% | (many diffs) |
| 8 | human | 254122 | SNX32 | sorting nexin 32 | XM_024448420.1 | 61.6% | 49.3% | (many diffs) |
| 9 | human | 254122 | SNX32 | sorting nexin 32 | XR_002957137.1 | 49.6% | (many diffs) | |
| 10 | human | 254122 | SNX32 | sorting nexin 32 | XR_001747822.2 | 48.4% | (many diffs) | |
| 11 | mouse | 225861 | Snx32 | sorting nexin 32 | NM_001024560.2 | 86.9% | 90.8% | (many diffs) |
| 12 | mouse | 225861 | Snx32 | sorting nexin 32 | XM_006531706.3 | 76.7% | 79.1% | (many diffs) |
| 13 | mouse | 225861 | Snx32 | sorting nexin 32 | XM_011248617.2 | 76.1% | 56.8% | (many diffs) |
| 14 | mouse | 225861 | Snx32 | sorting nexin 32 | XM_011248618.1 | 54.4% | 36.6% | (many diffs) |
| 15 | mouse | 225861 | Snx32 | sorting nexin 32 | XR_388254.3 | 51.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1275
- ORF length:
- 1209
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gacgtatgcg gaggttggca aggagggcaa gccttcctgt gcatcggtgg 121 atctgcaggg agacagctcc ttacaggtgg agatttctga cgcagtgagt gagcgggaca 181 aggtgaaatt cactgttcaa acaaagagct gcctccctca cttcgcccag accgagttct 241 cagtcgtgcg gcagcacgag gagttcatct ggctgcatga tgcctacgtg gagaatgagg 301 agtacgccgg cctcatcatc cccccagccc ctccgaggcc agactttgag gcttcgaggg 361 aaaagctaca gaaattgggc gagggggaca gctctgtcac tcgggaagag tttgccaaga 421 tgaagcagga gctggaagcg gagtacctgg ccatctttaa gaagacagtt gcgatgcacg 481 aagtctttct gcagcgcctg gcggcccacc ccaccctgcg tcgagaccac aacttctttg 541 tgtttttgga atatggacag gatctgagtg tccgggggaa gaacaggaag gagctcctcg 601 gagggtttct gaggaatatt gtgaagtccg cggatgaagc cctcatcacg ggcatgtcag 661 ggctcaagga ggtggatgac ttctttgagc atgagaggac cttcctgttg gagtatcaca 721 cccgtatccg agatgcctgc ctgcgggccg accgcgtcat gcgcgcccac aagtgcctgg 781 cagacgatta tatccctatc TCAGCTGCGC TGAGCAGTCT GGGAACACAG GAAGTCAACC 841 AGCTAAGGAC GAGCTTCCTC AAATTGGCAG AGCTCTTTGA ACGGCTGAGG AAGCTGGAGG 901 GCCGGGTGGC TTCCGATGAG GACCTGAAGC TGTCAGACAT GCTGAGGTAC TACATGCGTG 961 ACTCACAGGC AGCCAAGGAC CTGCTGTACC GGCGGCTGCG GGCACTGGCC GACTACGAGA 1021 ATGCCAACAA GGCGCTGGAC AAGGCGCGCA CCAGGAACCG GGAGGTGCGG CCCGCCGAGA 1081 GCCACCAGCA GCTGTGCTGC CAACGCTTCG AGCGCCTCTC CGACTCCGCC AAGCAAGAGC 1141 TCATGGACTT CAAGTCCCGC CGGGTCTCCT CTTTTCGAAA GAATCTCATT GAGCTGGCAG 1201 AGCTGGAGCT CAAACACGCC AAGGCCAGCA CCCTGATTCT CCGGAACACC CTTGTTGCCC 1261 TAAAGGGGGA GCCTTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1321 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGCCCGTAA CTTGAAAGTA 1381 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CGAGCCCGAA AGTTCCTGTA 1441 ATATACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt