Transcript: Mouse XM_006531706.3

PREDICTED: Mus musculus sorting nexin 32 (Snx32), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Snx32 (225861)
Length:
2108
CDS:
325..1680

Additional Resources:

NCBI RefSeq record:
XM_006531706.3
NBCI Gene record:
Snx32 (225861)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006531706.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000178635 CGGAATACTTGGCCATCTTTA pLKO.1 701 CDS 100% 13.200 9.240 N Snx32 n/a
2 TRCN0000200216 CCAGGAAGTCAACCAACTCAA pLKO.1 1089 CDS 100% 4.950 3.465 N Snx32 n/a
3 TRCN0000182592 GAAGAACAGGAAGGAGGTCTT pLKO.1 840 CDS 100% 4.050 2.835 N Snx32 n/a
4 TRCN0000182166 CCCTATAAAGACGCTCTCCTT pLKO.1 1833 3UTR 100% 2.640 1.848 N Snx32 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006531706.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_16135 pDONR223 0% 76.7% 79.1% None (many diffs) n/a
2 ccsbBroad304_16135 pLX_304 0% 76.7% 79.1% V5 (many diffs) n/a
3 TRCN0000471625 CGAGCCCGAAAGTTCCTGTAATAT pLX_317 39.4% 76.7% 79.1% V5 (many diffs) n/a
4 ccsbBroadEn_09895 pDONR223 100% 76.4% 78.4% None (many diffs) n/a
5 ccsbBroad304_09895 pLX_304 0% 76.4% 78.4% V5 (many diffs) n/a
6 TRCN0000467330 AACTCGTTTTTGTACTACGACACG pLX_317 33.2% 76.4% 78.4% V5 (many diffs) n/a
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