Construct: ORF TRCN0000471638
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016761.1_s317c1
- Derived from:
- ccsbBroadEn_12043
- DNA Barcode:
- CCCTCTGCGTCATTAGCAGCGTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RETREG1 (54463)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471638
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54463 | RETREG1 | reticulophagy regulator 1 | XM_024446117.1 | 43.7% | 43.7% | 1_543del |
| 2 | human | 54463 | RETREG1 | reticulophagy regulator 1 | XM_024446118.1 | 43.7% | 43.7% | 1_543del |
| 3 | human | 54463 | RETREG1 | reticulophagy regulator 1 | NM_019000.4 | 39.6% | 39.6% | 1_645del |
| 4 | human | 54463 | RETREG1 | reticulophagy regulator 1 | XM_011514055.3 | 38.7% | 38.7% | 1_669del |
| 5 | human | 54463 | RETREG1 | reticulophagy regulator 1 | XM_011514054.2 | 35.6% | 35.6% | 1_765del |
| 6 | human | 54463 | RETREG1 | reticulophagy regulator 1 | NM_001034850.2 | 28.3% | 28.3% | 1_1068del |
| 7 | human | 54463 | RETREG1 | reticulophagy regulator 1 | XM_011514053.3 | 26.2% | 26.2% | 1_1188del |
| 8 | mouse | 66270 | Retreg1 | reticulophagy regulator 1 | NM_001277318.1 | 37.1% | 37.8% | (many diffs) |
| 9 | mouse | 66270 | Retreg1 | reticulophagy regulator 1 | NM_001277317.1 | 34.5% | 35.2% | (many diffs) |
| 10 | mouse | 66270 | Retreg1 | reticulophagy regulator 1 | NM_001277315.1 | 33.5% | 34.2% | (many diffs) |
| 11 | mouse | 66270 | Retreg1 | reticulophagy regulator 1 | NM_025459.3 | 33.5% | 34.2% | (many diffs) |
| 12 | mouse | 66270 | Retreg1 | reticulophagy regulator 1 | XM_006520134.3 | 33.5% | 34.2% | (many diffs) |
| 13 | mouse | 66270 | Retreg1 | reticulophagy regulator 1 | XM_017316720.1 | 33.5% | 34.2% | (many diffs) |
| 14 | mouse | 66270 | Retreg1 | reticulophagy regulator 1 | NM_001277316.1 | 32.8% | 33.5% | (many diffs) |
| 15 | mouse | 66270 | Retreg1 | reticulophagy regulator 1 | NM_001034851.2 | 24.9% | 25.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 489
- ORF length:
- 423
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttG TACAAAAAAG 61 TTGGCATGGG AACAAATGAT GAAGATGAAT TAAGCCTTGG TTTGCCCACT GAGCTCAAGA 121 GAAAGAAGGA ACAGTTGGAC AGTGGTCACA GACCAAGCAA AGAGACGCAA TCAGCAGCTG 181 GTCTCACCCT TCCTCTGAAC AGTGACCAAA CCTTTCACCT GATGAGCAAC CTGGCTGGGG 241 ATGTTATCAC AGCTGCAGTG ACTGCAGCTA TCAAAGACCA GTTAGAGGGT GTGCAGCAAG 301 CACTTTCTCA GGCTGCCCCC ATCCCAGAAG AGGACACAGA CACTGAAGAA GGTGATGACT 361 TTGAACTACT TGACCAGTCA GAGCTGGATC AAATTGAGAG TGAATTGGGA CTTACACAAG 421 ACCAGGAAGC AGAAGCACAG CAAAATAAGA AGTCTTCAGG TTTCCTTTCA AATCTGCTGG 481 GAGGCCATTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 541 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 601 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCCTCT GCGTCATTAG CAGCGTTAAC 661 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt