Construct: ORF TRCN0000471639
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004572.1_s317c1
- Derived from:
- ccsbBroadEn_06151
- DNA Barcode:
- GACATCCACCACTGACTAGTGAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF5 (1983)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471639
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1983 | EIF5 | eukaryotic translation init... | NM_001969.5 | 99.9% | 99.7% | 18C>G |
| 2 | human | 1983 | EIF5 | eukaryotic translation init... | NM_183004.5 | 99.9% | 99.7% | 18C>G |
| 3 | mouse | 217869 | Eif5 | eukaryotic translation init... | NM_173363.5 | 92.3% | 96.9% | (many diffs) |
| 4 | mouse | 217869 | Eif5 | eukaryotic translation init... | NM_178041.2 | 92.3% | 96.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1359
- ORF length:
- 1293
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgtcaatgtc aagcgcagcg tgtcagacca gttctatcgc tacaagatgc 121 cccgtctgat tgccaaggtt gagggcaaag gcaatggaat caagacagtt atagtcaaca 181 tggttgacgt tgcaaaggcg cttaatcggc ctccaacgta tcccaccaaa tattttggtt 241 gtgagctggg agcacagacc cagtttgatg ttaagaatga ccgttacatt gtcaatggat 301 ctcatgaggc gaataagctg caagacatgt tggatggatt cattaaaaaa tttgttctct 361 gtcctgaatg tgagaatcct gaaacagatt tgcatgtcaa tccaaagaag caaacaatag 421 gtaattcttg taaagcctgt ggctatcgag gcatgcttga cacacatcat aaactctgca 481 cattcattct caaaaaccca cctgagaata gtgacagtgg tacaggaaag aaagaaaaag 541 aaaagaaaaa cagaaagggc aaagacaagg aaaatggctc cgtatccagc agtgagacac 601 caccaccacc accaccacca aatgaaatta atcctcctcc acatacaatg gaagaagagg 661 aggatgatga ctggggagaa gatacaactg aggaagctca aaggcgtcga atggatgaaa 721 tcagtgacca tgcaaaagtt ctgacactca gtgatgattt ggaaagaaca attgaggaga 781 gggtcaatat cctctttgat tttgttaaga aaaagaaaga agagggtgtt attgattcat 841 cTGACAAAGA AATCGTTGCT GAAGCAGAAA GACTGGATGT AAAAGCCATG GGCCCTCTTG 901 TTCTAACTGA AGTTCTTTTT AATGAGAAGA TTAGAGAACA GATTAAGAAA TACAGGCGCC 961 ATTTCCTACG ATTTTGTCAC AACAACAAAA AAGCCCAACG GTACCTTCTT CATGGTTTGG 1021 AGTGTGTGGT AGCAATGCAT CAAGCTCAGC TTATCTCCAA GATTCCACAT ATCTTGAAGG 1081 AGATGTACGA TGCAGACCTT TTAGAAGAAG AGGTCATCAT CAGCTGGTCG GAAAAGGCCT 1141 CTAAGAAATA TGTCTCCAAA GAACTTGCCA AAGAGATTCG TGTCAAAGCA GAACCATTTA 1201 TAAAATGGTT GAAGGAGGCA GAGGAAGAAT CTTCTGGTGG CGAAGAAGAA GATGAAGATG 1261 AGAACATTGA GGTGGTGTAT TCGAAGGCTG CCAGTGTACC GAAAGTTGAG ACTGTAAAGT 1321 CAGACAACAA GGATGACGAC ATCGATATTG ATGCCATTTA CCCAACTTTC TTGTACAAAG 1381 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1441 GAACTAGTCC GTAACTTGAA AGTATTTCGA TCTTGGCTTT ATATATCTTG TGGAAAGGAC 1501 GAGACATCCA CCACTGACTA GTGAGCACGC GTTAAGTCga caatcaacct ctggattaca 1561 aaatttgtga aagatt