Construct: ORF TRCN0000471647
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018996.1_s317c1
- Derived from:
- ccsbBroadEn_14694
- DNA Barcode:
- TCGGTCCAATATTTAGTTCTGCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MATK (4145)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471647
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4145 | MATK | megakaryocyte-associated ty... | NM_139355.3 | 100% | 100% | |
| 2 | human | 4145 | MATK | megakaryocyte-associated ty... | XM_011528018.1 | 100% | 100% | |
| 3 | human | 4145 | MATK | megakaryocyte-associated ty... | NM_002378.4 | 95.2% | 94% | (many diffs) |
| 4 | human | 4145 | MATK | megakaryocyte-associated ty... | NM_139354.3 | 91.9% | 91.9% | 0_1ins123 |
| 5 | mouse | 17179 | Matk | megakaryocyte-associated ty... | XM_006513294.3 | 83.8% | 87.3% | (many diffs) |
| 6 | mouse | 17179 | Matk | megakaryocyte-associated ty... | NM_010768.2 | 83.7% | 87.3% | (many diffs) |
| 7 | mouse | 17179 | Matk | megakaryocyte-associated ty... | XM_006513295.3 | 83.7% | 87.3% | (many diffs) |
| 8 | mouse | 17179 | Matk | megakaryocyte-associated ty... | XM_011243376.2 | 81.1% | 85% | (many diffs) |
| 9 | mouse | 17179 | Matk | megakaryocyte-associated ty... | XM_011243377.2 | 81.1% | 85% | (many diffs) |
| 10 | mouse | 17179 | Matk | megakaryocyte-associated ty... | NM_001285853.1 | 77.8% | 82.4% | (many diffs) |
| 11 | mouse | 17179 | Matk | megakaryocyte-associated ty... | XM_006513298.3 | 77.8% | 82.4% | (many diffs) |
| 12 | mouse | 17179 | Matk | megakaryocyte-associated ty... | XM_006513300.2 | 77.8% | 82.4% | (many diffs) |
| 13 | mouse | 17179 | Matk | megakaryocyte-associated ty... | NM_001285854.1 | 77.8% | 82.4% | (many diffs) |
| 14 | mouse | 17179 | Matk | megakaryocyte-associated ty... | NM_001285855.1 | 77.8% | 82.4% | (many diffs) |
| 15 | mouse | 17179 | Matk | megakaryocyte-associated ty... | XM_006513299.2 | 77.8% | 82.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1590
- ORF length:
- 1521
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggggcga ggctctctgg tttcctggcg ggcatttcac ggctgtgatt 121 ctgctgagga acttccccgg gtgagccccc gcttcctccg agcctggcac ccccctcccg 181 tctcagccag gatgccaacg aggcgctggg ccccgggcac ccagtgtatc accaaatgcg 241 agcacacccg ccccaagcca ggggagctgg ccttccgcaa gggcgacgtg gtcaccatcc 301 tggaggcctg cgagaacaag agctggtacc gcgtcaagca ccacaccagt ggacaggagg 361 ggctgctggc agctggggcg ctgcgggagc gggaggccct ctccgcagac cccaagctca 421 gcctcatgcc gtggttccac gggaagatct cgggccagga ggctgtccag cagctgcagc 481 ctcccgagga tgggctgttc ctggtgcggg agtccgcgcg ccaccccggc gactacgtcc 541 tgtgcgtgag ctttggccgc gacgtcatcc actaccgcgt gctgcaccgc gacggccacc 601 tcacaatcga tgaggccgtg ttcttctgca acctcatgga catggtggag cattacagca 661 aggacaaggg cgctatctgc accaagctgg tgagaccaaa gcggaaacac gggaccaagt 721 cggccgagga ggagctggcc agggcgggct ggttactgaa cctgcagcat ttgacattgg 781 gagcacagat cggagaggga gagtttggag ctgtcctgca gggtgagtac ctggggcaaa 841 aggtggccgt gaagaatatc aagtgtgatg tgacagccca ggccttcctg gacgagacgg 901 ccgtcatgac gaagatgcaa cacgagaacc tggtgcgtct cctgggcgtg atcctgcacc 961 aggggctgta cattgtcatg gagcacgtga gcaagggcaa cctggtgaac tttctgcgga 1021 cccggggtcg agccctcgtg aacaccgctc agctcctgca gttttctctg cacgtggccg 1081 agggcatgga gtacctggag agcaagaagc ttgtgcaccg cgacctggcc gcccgcaaca 1141 tcctggtctc agaggaccTG GTGGCCAAGG TCAGCGACTT TGGCCTGGCC AAAGCCGAGC 1201 GGAAGGGGCT AGACTCAAGC CGGCTGCCCG TCAAGTGGAC GGCGCCCGAG GCTCTCAAAC 1261 ACGGGAAGTT CACCAGCAAG TCGGATGTCT GGAGTTTTGG GGTGCTGCTC TGGGAGGTCT 1321 TCTCATATGG ACGGGCTCCG TACCCTAAAA TGTCACTGAA AGAGGTGTCG GAGGCCGTGG 1381 AGAAGGGGTA CCGCATGGAA CCCCCCGAGG GCTGTCCAGG CCCCGTGCAC GTCCTCATGA 1441 GCAGCTGCTG GGAGGCAGAG CCCGCCCGCC GGCCACCCTT CCGCAAACTG GCCGAGAAGC 1501 TGGCCCGGGA GCTACGCAGT GCAGGTGCCC CAGCCTCCGT CTCAGGGCAG GACGCCGACG 1561 GCTCCACCTC GCCCCGAAGC CAGGAGCCCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1621 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1681 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCGGTCCA 1741 ATATTTAGTT CTGCTTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1801 aagatt