Construct: ORF TRCN0000471655
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018950.3_s317c1
- Derived from:
- ccsbBroadEn_14817
- DNA Barcode:
- TTTCTTGAACGCGGACCGTGTCTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PSKH1 (5681)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471655
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5681 | PSKH1 | protein serine kinase H1 | NM_006742.3 | 99.6% | 38.7% | (many diffs) |
| 2 | mouse | 244631 | Pskh1 | protein serine kinase H1 | NM_173432.2 | 88.3% | 36.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 612
- ORF length:
- 543
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggctgtggg acaagcaagg tccttcccga gccacccaag gatgtccagc 121 tggatctggt caagaaggtg gagcccttca gtggcactaa gagtgacgtg tacaagcact 181 tcatcacaga ggtggacagt gttggccctg tcaaagccgg gttcccagca gcaagtcagt 241 atgcacaccc ctgccccggt cccccgactg ctggccacac ggagcctccc tcagaaccac 301 cacgcagggc cagggtagct aagtacaggg ccaagtttga cccacgtgtt acagctaagt 361 atgacatcaa ggccctaatt ggccgaggca gcttcagccg agtggtacgt gtagagcacc 421 gggcaacccg gcagccgtat gccatcaaga tgattgagac caagtaccgg gaggggcggg 481 aggtgtgtga gtcggagctg cgtgtgctgc gtcgggtgcg tcatgccaac atcatccagc 541 tggtggaggt gttcgagaca caggagcggg tgttacatgg tgatggagct ggccactggt 601 ggagagctct ttgaccgcat cattgccaag ggctccttca ccgagcgtga cgccacgcgg 661 gtgctgcaga tggtgctgga tggcgtccgg tatctgcatg cactgggcat cacacaccga 721 gacctcaaac ntgagaatct gctctactac catcccgggc actgactcca agatcatcat 781 caccgacttc ggcctggcca gtgctcgcaa gaagggtgga tgactgcntg atgaagacca 841 cctgtggcac gcctgagtac attgccccag aagtcctggt ccgcaagcca tacaccaact 901 cagtggacat gtgggcgctg ggcgtcattg cctacatcct actcagtggc accatgccgt 961 ttGAGGATGA CAACCGTACC CGGCTGTACC GGCAGATCCT CAGGGGCAAG TACAGTTACT 1021 CTGGGGAGCC CTGGCCTAGT GTGTCCAACC TGGCCAAGGA CTTCATTGAC CGCCTGCTGA 1081 CAGTGGACCC TGGAGCCCGT ATGACTGCAC TGCAGGCCCT GAGGCACCCG TGGGTGGTGA 1141 GCATGGCTGC CTCTTCATCC ATGAAGAACC TGCACCGCTC CATATCCCAG AACCTCCTTA 1201 AACGTGCCTC CTCGCGCTGC CAGAGCACCA AATCTGCCCA GTCCACGCGT TCCAGCCGCT 1261 CCACACGCTC CAATAAGTCA CGCCGTGTGC GGGAACGGGA GCTGCGGGAG CTCAACCTGC 1321 GCTACCAGCA GCAATACAAT GGCTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1381 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1441 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TTCTTGAACG 1501 CGGACCGTGT CTCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1561 att