Construct: ORF TRCN0000471659
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002114.3_s317c1
- Derived from:
- ccsbBroadEn_02108
- DNA Barcode:
- GTACGGTTTCTTGAGACCCTTTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AURKB (9212)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471659
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9212 | AURKB | aurora kinase B | NM_001313950.2 | 100% | 100% | |
| 2 | human | 9212 | AURKB | aurora kinase B | NM_004217.4 | 100% | 100% | |
| 3 | human | 9212 | AURKB | aurora kinase B | NM_001284526.2 | 99.7% | 99.7% | 207_209delCAG |
| 4 | human | 9212 | AURKB | aurora kinase B | NM_001313953.3 | 88.9% | 87.2% | (many diffs) |
| 5 | human | 9212 | AURKB | aurora kinase B | NM_001256834.3 | 88% | 88% | 0_1ins123 |
| 6 | human | 9212 | AURKB | aurora kinase B | NM_001313951.1 | 88% | 88% | 0_1ins123 |
| 7 | human | 9212 | AURKB | aurora kinase B | XM_011524072.3 | 88% | 88% | 0_1ins123 |
| 8 | human | 9212 | AURKB | aurora kinase B | XM_017025307.2 | 88% | 88% | 0_1ins123 |
| 9 | human | 9212 | AURKB | aurora kinase B | NM_001313952.2 | 87.8% | 87.8% | 0_1ins123;84_86delCAG |
| 10 | human | 9212 | AURKB | aurora kinase B | XM_017025308.2 | 77% | 75.2% | (many diffs) |
| 11 | human | 9212 | AURKB | aurora kinase B | NR_132731.1 | 74.6% | (many diffs) | |
| 12 | human | 9212 | AURKB | aurora kinase B | NR_132730.2 | 71.6% | 1_71del;327_328ins142;962_1101del | |
| 13 | human | 9212 | AURKB | aurora kinase B | NM_001313954.2 | 54.3% | 49.2% | 0_1ins466;71_80delGGTCCGGGCG |
| 14 | human | 9212 | AURKB | aurora kinase B | XM_017025309.1 | 54.3% | 49.2% | 0_1ins466;71_80delGGTCCGGGCG |
| 15 | human | 9212 | AURKB | aurora kinase B | XM_017025310.1 | 54.3% | 49.2% | 0_1ins466;71_80delGGTCCGGGCG |
| 16 | human | 9212 | AURKB | aurora kinase B | XM_017025311.1 | 54.3% | 49.2% | 0_1ins466;71_80delGGTCCGGGCG |
| 17 | human | 9212 | AURKB | aurora kinase B | NM_001313955.2 | 50.2% | 47.5% | (many diffs) |
| 18 | mouse | 20877 | Aurkb | aurora kinase B | NM_011496.2 | 81.3% | 82.9% | (many diffs) |
| 19 | mouse | 20877 | Aurkb | aurora kinase B | XM_006532725.3 | 53.5% | 55% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1098
- ORF length:
- 1032
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccagaaggag aactcctacc cctggcccta cggccgacag acggctccat 121 ctggcctgag caccctgccc cagcgagtcc tccggaaaga gcctgtcacc ccatctgcac 181 ttgtcctcat gagccgctcc aatgtccagc ccacagctgc ccctggccag aaggtgatgg 241 agaatagcag tgggacaccc gacatcttaa cgcggcactt cacaattgat gactttgaga 301 ttgggcgtcc tctgggcaaa ggcaagtttg gaaacgtgta cttggctcgg gagaagaaaa 361 gccatttcat cgtggcgctc aaggtcctct tcaagtccca gatagagaag gagggcgtgg 421 agcatcagct gcgcagagag atcgaaatcc aggcccacct gcaccatccc aacatcctgc 481 gtctctacaa ctatttttat gaccggagga ggatctactt gattctagag tatgcccccc 541 gcggggagct ctacaaggag ctgcagaaga gctgcacatt tgacgagcag cgaacagcca 601 cgatcatgga ggagttggca gatgctctaa tgtactgcca tgggaagaag gtgattcaca 661 gagacataaa gccagaaaat ctgcTCTTAG GGCTCAAGGG AGAGCTGAAG ATTGCTGACT 721 TCGGCTGGTC TGTGCATGCG CCCTCCCTGA GGAGGAAGAC AATGTGTGGC ACCCTGGACT 781 ACCTGCCCCC AGAGATGATT GAGGGGCGCA TGCACAATGA GAAGGTGGAT CTGTGGTGCA 841 TTGGAGTGCT TTGCTATGAG CTGCTGGTGG GGAACCCACC CTTTGAGAGT GCATCACACA 901 ACGAGACCTA TCGCCGCATC GTCAAGGTGG ACCTAAAGTT CCCCGCTTCC GTGCCCATGG 961 GAGCCCAGGA CCTCATCTCC AAACTGCTCA GGCATAACCC CTCGGAACGG CTGCCCCTGG 1021 CCCAGGTCTC AGCCCACCCT TGGGTCCGGG CCAACTCTCG GAGGGTGCTG CCTCCCTCTG 1081 CCCTTCAATC TGTCGCCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1141 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1201 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGTACGGT TTCTTGAGAC 1261 CCTTTACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt