Transcript: Human NM_001256834.3

Homo sapiens aurora kinase B (AURKB), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-16
Taxon:
Homo sapiens (human)
Gene:
AURKB (9212)
Length:
1170
CDS:
122..1033

Additional Resources:

NCBI RefSeq record:
NM_001256834.3
NBCI Gene record:
AURKB (9212)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001149202 CATCAACCCATACTGCAGGT pXPR_003 GGG 271 30% 4 0.6392 AURKB AURKB 77617
2 BRDN0001146837 TGACGAGCAGCGAACAGCCA pXPR_003 CGG 409 45% 5 0.5338 AURKB AURKB 77619
3 BRDN0001146267 ATTCTAGAGTATGCCCCCCG pXPR_003 CGG 350 38% 5 0.1546 AURKB AURKB 77618
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001256834.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000000777 CCTGCGTCTCTACAACTATTT pLKO.1 409 CDS 100% 13.200 18.480 N AURKB n/a
2 TRCN0000344504 CCTGCGTCTCTACAACTATTT pLKO_005 409 CDS 100% 13.200 18.480 N AURKB n/a
3 TRCN0000000776 CTACCTCCTCCTTTGTTTAAT pLKO.1 1126 3UTR 100% 15.000 12.000 N AURKB n/a
4 TRCN0000344570 CTACCTCCTCCTTTGTTTAAT pLKO_005 1126 3UTR 100% 15.000 12.000 N AURKB n/a
5 TRCN0000219657 TTAACGCGGCACTTCACAATT pLKO.1 200 CDS 100% 13.200 9.240 N AURKB n/a
6 TRCN0000332971 TTAACGCGGCACTTCACAATT pLKO_005 200 CDS 100% 13.200 9.240 N AURKB n/a
7 TRCN0000195436 CATCGTCAAGGTGGACCTAAA pLKO.1 850 CDS 100% 10.800 7.560 N AURKB n/a
8 TRCN0000199233 CCATCTGCACTTGTCCTCATG pLKO.1 104 5UTR 100% 4.050 2.835 N AURKB n/a
9 TRCN0000000779 GAAGAGCTGCACATTTGACGA pLKO.1 499 CDS 100% 2.640 1.848 N AURKB n/a
10 TRCN0000353008 GAAGAGCTGCACATTTGACGA pLKO_005 499 CDS 100% 2.640 1.848 N AURKB n/a
11 TRCN0000000778 TGATGGAGAATAGCAGTGGGA pLKO.1 168 CDS 100% 0.660 0.462 N AURKB n/a
12 TRCN0000199198 CAGCGAGTCCTCCGGAAAGAG pLKO.1 74 5UTR 100% 0.000 0.000 N AURKB n/a
13 TRCN0000353066 CAGCGAGTCCTCCGGAAAGAG pLKO_005 74 5UTR 100% 0.000 0.000 N AURKB n/a
14 TRCN0000010547 GCATCACACAACGAGACCTAT pLKO.1 824 CDS 100% 4.950 2.970 N AURKB n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001256834.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02108 pDONR223 100% 88% 88% None 0_1ins123 n/a
2 ccsbBroad304_02108 pLX_304 0% 88% 88% V5 0_1ins123 n/a
3 TRCN0000471659 GTACGGTTTCTTGAGACCCTTTAC pLX_317 40.3% 88% 88% V5 0_1ins123 n/a
4 ccsbBroadEn_14932 pDONR223 0% 88% 88% None 0_1ins123 n/a
5 ccsbBroad304_14932 pLX_304 0% 88% 88% V5 0_1ins123 n/a
6 TRCN0000474845 GGCTCCACACATCCACTGCTTAGC pLX_317 48.2% 88% 88% V5 0_1ins123 n/a
7 TRCN0000488915 ATCAAATATCTTTTAGGCTAAAAC pLX_317 34.3% 87.8% 87.7% V5 0_1ins123;770T>C;894C>T n/a
8 TRCN0000489191 CTGATGCCGCCGCGGCGTTTTATT pLX_317 34.3% 87.8% 87.7% V5 (not translated due to prior stop codon) 0_1ins123;762C>T;770T>C n/a
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