Construct: ORF TRCN0000471680
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017215.1_s317c1
- Derived from:
- ccsbBroadEn_07366
- DNA Barcode:
- GGCCAAACTTTGCAATACTTCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC6A5 (9152)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471680
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9152 | SLC6A5 | solute carrier family 6 mem... | NM_004211.5 | 99.7% | 99.7% | (many diffs) |
| 2 | human | 9152 | SLC6A5 | solute carrier family 6 mem... | NM_001318369.2 | 70.5% | 70.6% | (many diffs) |
| 3 | human | 9152 | SLC6A5 | solute carrier family 6 mem... | XM_017018544.2 | 63.2% | 63.3% | (many diffs) |
| 4 | human | 9152 | SLC6A5 | solute carrier family 6 mem... | XM_017018545.2 | 56.3% | 56.4% | 0_1ins1041;189A>G;1062G>A |
| 5 | mouse | 104245 | Slc6a5 | solute carrier family 6 (ne... | NM_001146013.1 | 88.5% | 94% | (many diffs) |
| 6 | mouse | 104245 | Slc6a5 | solute carrier family 6 (ne... | NM_148931.3 | 87.5% | 93% | (many diffs) |
| 7 | mouse | 104245 | Slc6a5 | solute carrier family 6 (ne... | XM_006540547.1 | 87.5% | 93% | (many diffs) |
| 8 | mouse | 104245 | Slc6a5 | solute carrier family 6 (ne... | XM_006540546.3 | 86.8% | 92.1% | (many diffs) |
| 9 | mouse | 104245 | Slc6a5 | solute carrier family 6 (ne... | XM_006540545.3 | 85% | 90.2% | (many diffs) |
| 10 | mouse | 104245 | Slc6a5 | solute carrier family 6 (ne... | XM_006540548.2 | 72.4% | 76.3% | (many diffs) |
| 11 | mouse | 104245 | Slc6a5 | solute carrier family 6 (ne... | XM_006540549.3 | 63.7% | 69.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2460
- ORF length:
- 2391
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggattgcagt gctcccaagg aaatgaataa actgccagcc aacagcccgg 121 aggcggcggc ggcgcagggc cacccggatg gcccatgcgc tcccaggacg agcccggagc 181 aggagcttcc cgcggctgcc gccccgccgc cgccacgtgt gcccaggtcc gcttccaccg 241 gcgcccaaac tttccagtca gcggacgcgc gagcctgcga ggctgagcgg ccaggagtgg 301 ggtcttgcaa actcagtagc ccgcgggcgc aggcggcctc tgcagctctg cgggacttga 361 gagaggcgca aggcgcgcag gcctcgcccc ctcccgggag ctccgggccc ggcaacgcgt 421 tgcactgtaa gatcccttct ctgcgaggcc cggaggggga tgcgaacgtg agtgtgggca 481 agggcaccct ggagcggaac aatacccctg ttgtgggctg ggtgaacatg agccagagca 541 ccgtggtgct gggcacggat ggaatcacgt ccgtgctccc gggcagcgtg gccaccgttg 601 ccacccagga ggacgagcaa ggggatgaga ataaggcccg agggaactgg tccagcaaac 661 tggacttcat cctgtccatg gtggggtacg cagtggggct gggcaatgtc tggaggtttc 721 cctacctggc cttccagaac gggggaggtg ctttcctcat cccttacctg atgatgctgg 781 ctctggctgg attacccatc ttcttcttgg aggtgtcgct gggccagttt gccagccagg 841 gaccagtgtc tgtgtggaag gccatcccag ctctacaagg ctgtggcatc gcgatgctga 901 tcatctctgt cctaatagcc atatactaca atgtgattat ttgctataca cttttctacc 961 tgtttgcctc ctttgtgtct gtactaccct ggggctcctg caacaaccct tggaatacac 1021 cagaatgcaa agataaaacc aaacttttat tagattcctg tgttatcagt gaccatccca 1081 aaatacagat caagaactcg actttctgca tgaccgctta tcccaacgtg acaatggtta 1141 atttcaccag ccaggccaat aagacatttg tcagtggaag tgaagagtac ttcaagtact 1201 ttgtgctgaa gatttctgca gggattgaat atcctggcga gatcaggtgg ccactagctc 1261 tctgcctctt cctggcttgg gtcattgtgt atgcatcgtt ggctaaagga atcaagactt 1321 caggaaaagt ggtgtacttc acggccacgt tcccgtatgt cgtactcgtg atcctcctca 1381 tccgaggagt caccctgcct ggagctggag ctgggatctg gtacttcatc acacccaagt 1441 gggagaaact cacggatgcc acggtgtgga aagatgctgc cactcagatt ttcttctctt 1501 tatctgctgc atggggaggc ctgatcactc tctcttctta caacaaattc cacaacaact 1561 gctacaggga cactctaatt gtcacctgca ccaacagtgc cacaagcatc tttgccggct 1621 tcgtcatctt ctccgttatc ggcttcatgg ccaatgaacg caaagtcaac attgagaatg 1681 tggcagacca agggccaggc attgcatttg tggtttaccc ggaagcctta accaggctgc 1741 ctctctctcc gttctgggcc atcatctttt tcctgatgct cctcactctt ggacttgaca 1801 ctatgtttgc caccatcgag accatagtga cctccatctc agacgagttt cccaagtacc 1861 tacgcacaca caagccagtg tttactctgg gctgctgcat ttgtttcttc atcatgggtt 1921 ttccaatgat cactcagggt ggaatttaca tgtttcagct tgtggacacc tatgctgcct 1981 cctatgccct tgtcatcatt gccatttttg agctcgtggg gatctcttat gtgtatggct 2041 tgcaaagatt ctgtgaagat atagagatga tgattggatt ccagcctaac atcttctgga 2101 aagtctgctg ggcatttgta accccaacca ttttaacctt tatcctttgc ttcagctttt 2161 accagtggga acccatgacc tatggctcTT ACCGCTATCC TAACTGGTCC ATGGTGCTCG 2221 GATGGCTAAT GCTCGCCTGT TCCGTCATCT GGATCCCAAT TATGTTTGTG ATAAAAATGC 2281 ATCTGGCCCC TGGAAGATTT ATTGAGAGGC TGAAGTTGGT GTGCTCGCCA CAGCCGGACT 2341 GGGGCCCATT CTTAGCTCAA CACCGCGGGG AGCGTTACAA GAACATGATC GACCCCTTGG 2401 GAACCTCTTC CTTGGGACTC AAACTGCCAG TGAAGGATTT GGAACTGGGC ACTCAGTGCT 2461 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2521 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAAGTATTTC GATTTCTTGG 2581 CTTTATATAT CTTGTGGAAA GGACGAGGCC AAACTTTGCA ATACTTCTTT ACGCGTTAAG 2641 TCgacaatca acctctggat tacaaaattt gtgaaagatt