Construct: ORF TRCN0000471681
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014629.1_s317c1
- Derived from:
- ccsbBroadEn_06967
- DNA Barcode:
- AACAGATAGCAAAAAACGACCTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC15A1 (6564)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471681
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6564 | SLC15A1 | solute carrier family 15 me... | NM_005073.4 | 99.9% | 99.8% | 952C>G;1347T>C |
| 2 | mouse | 56643 | Slc15a1 | solute carrier family 15 (o... | NM_053079.2 | 83.3% | 85% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2193
- ORF length:
- 2124
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggaatgtcc aaatcacaca gtttctttgg ttatcccctg agcatcttct 121 tcatcgtggt caatgagttt tgcgaaagat tttcctacta tggaatgcga gcaatcctga 181 ttctgtactt cacaaatttc atcagctggg atgataacct gtccaccgcc atctaccata 241 cgtttgtggc tctgtgctac ctgacgccaa ttctcggagc tcttatcgcc gactcgtggc 301 tgggaaagtt caagaccatt gtgtcgctct ccattgtcta cacaattgga caagcagtca 361 cctcagtaag ctccattaat gacctcacag accacaacca tgatggcacc cccgacagcc 421 ttcctgtgca cgtggtgctg tccttgatcg gcctggccct gatagctctc gggactggag 481 gaatcaaacc ctgtgtgtct gcgtttggtg gagatcagtt tgaagagggc caggagaaac 541 aaagaaacag atttttttcc atcttttact tggctattaa tgctggaagt ttgctttcca 601 caatcatcac acccatgctc agagttcaac aatgtggaat tcacagtaaa caagcttgtt 661 acccactggc ctttggggtt cctgctgctc tcatggctgt agccctgatt gtgtttgtcc 721 ttggcagtgg gatgtacaag aagttcaagc cacagggcaa catcatgggt aaagtggcca 781 agtgcatcgg ttttgccatc aaaaatagat ttaggcatcg gagtaaggca tttcccaaga 841 gggagcactg gctggactgg gctaaagaga aatacgatga gcggctcatc tcccaaatta 901 agatggttac gagggtgatg ttcctgtata ttccactccc aatgttctgg gccttgtttg 961 accagcaggg ctccaggtgg acactgcagg caacaactat gtccgggaaa atcggagctg 1021 ttgaaattca gcccgatcag atgcagaccg tgaacgccat cctgatcgtg atcatggtcc 1081 cgatcttcga tgctgtgctg taccctctca ttgcaaaatg tggcttcaat ttcacctcct 1141 tgaagaagat ggcagttggc atggtcctgg cctccatggc ctttgtggtg gctgccatcg 1201 tgcaggtgga aatcgataaa actcttccag tcttccccaa aggaaacgaa gtccaaatta 1261 aagttttgaa tataggaaac aataccatga atatatctct tcctggagag atggtgacac 1321 ttggcccaat gtctcaaaca aatgcattta tgacttttga tgtaaacaaa ctgacaagga 1381 taaacatttc ttctcctgga tcaccagtca ctgccgtaac tgacgacttc aagcagggcc 1441 aacgccacac gcttctagtg tgggccccca atcactacca ggtggtaaag gatggtctta 1501 accagaagcc agaaaaaggg gaaaatggaa tcagatttgt aaatactttt aacgagctca 1561 tcaccatcac aatgagtggg aaagtttatg caaacatcag cagctacaat gccagcacat 1621 accagttttt tccttctggc ataaaaggct tcacaataag ctcaacagag attccgccac 1681 aatgtcaacc taatttcaat actttctacc ttgaatttgg tagtgcttat acctatatag 1741 tccaaaggaa gaatgacagc tgccctgaag tgaaggtgtt tGAAGATATT TCAGCCAACA 1801 CAGTTAACAT GGCTCTGCAA ATCCCGCAGT ATTTTCTTCT CACCTGTGGC GAAGTGGTCT 1861 TCTCTGTCAC GGGATTGGAA TTCTCATATT CTCAGGCTCC TTCCAACATG AAGTCGGTGC 1921 TTCAGGCAGG ATGGCTGCTG ACCGTGGCTG TTGGCAACAT CATTGTGCTC ATCGTGGCAG 1981 GGGCAGGCCA GTTCAGCAAA CAGTGGGCCG AGTACATTCT ATTTGCCGCG TTGCTTCTGG 2041 TCGTCTGTGT AATTTTTGCC ATCATGGCTC GGTTCTATAC TTACATCAAC CCAGCGGAGA 2101 TCGAAGCTCA ATTTGATGAG GATGAAAAGA AAAACAGACT GGAAAAGAGT AACCCATATT 2161 TCATGTCAGG GGCCAATTCA CAGAAACAGA TGTTGCCAAC TTTCTTGTAC AAAGTGGTTG 2221 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2281 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA 2341 CAGATAGCAA AAAACGACCT CTACGCGTTA AGTCgacaat caacctctgg attacaaaat 2401 ttgtgaaaga tt