Construct: ORF TRCN0000471683
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005943.1_s317c1
- Derived from:
- ccsbBroadEn_12931
- DNA Barcode:
- GTTCGCATTGACTCGACCACCATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRT8P12 (90133)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471683
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3856 | KRT8 | keratin 8 | NM_001256293.2 | 22.2% | 17.5% | (many diffs) |
| 2 | human | 3856 | KRT8 | keratin 8 | NM_002273.4 | 22.2% | 17.5% | (many diffs) |
| 3 | human | 3856 | KRT8 | keratin 8 | NM_001256282.2 | 21% | 16.5% | (many diffs) |
| 4 | human | 283102 | KRT8P41 | keratin 8 pseudogene 41 | NR_027713.1 | 16% | (many diffs) | |
| 5 | human | 3856 | KRT8 | keratin 8 | NR_045962.2 | 15% | (many diffs) | |
| 6 | mouse | 16691 | Krt8 | keratin 8 | NM_031170.2 | 20.4% | 16.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 444
- ORF length:
- 351
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGCGAGG CATCACCACC ATCACGGTAA 121 ACCAGAGCCT GCTGAGCCCC CTTAACCTGG AGGTGGACTC CAACATTCAG GCTGTATACA 181 CCCAGGAGAA GGAGCAGATC AAGACCCTCA ACAACAAGTT TGCCTCCTTC ACAGACCAGG 241 TATGGTTCCT GGAGCAGCAG AACAAGGTGC TGGAGACCAA GTGGAGCCTT CTGCAGCAGC 301 ACAAGATGGC TGGGAGCAAC ATGGACAACA TGTTCGGGAG CTATATCAAC AACCTTAGGC 361 GGCTGCTGGA GCCAGGAGAA GCTGAGGCTG GAAGCGGAGC TTGGCAACAT GCAGGGGCTG 421 GTGGAGGACT TCAGGAACAA GTATTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 481 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 541 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG TTCGCATTGA 601 CTCGACCACC ATGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 661 att