Construct: ORF TRCN0000471706
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012557.1_s317c1
- Derived from:
- ccsbBroadEn_06268
- DNA Barcode:
- TCATGCTTAGTCCCCTTTCGGGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GCSH (2653)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471706
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2653 | GCSH | glycine cleavage system pro... | NM_004483.5 | 99.8% | 99.4% | 62C>T |
| 2 | human | 2653 | GCSH | glycine cleavage system pro... | XM_017023136.2 | 96.4% | 96% | 62C>T;424_441del |
| 3 | human | 2653 | GCSH | glycine cleavage system pro... | XM_017023137.1 | 77.4% | 72.2% | (many diffs) |
| 4 | human | 729080 | LOC729080 | glycine cleavage system pro... | NR_033244.1 | 46.8% | (many diffs) | |
| 5 | human | 641746 | LOC641746 | glycine cleavage system pro... | NR_033245.1 | 41.2% | (many diffs) | |
| 6 | human | 100329109 | GCSHP3 | glycine cleavage system pro... | NR_033248.1 | 32.4% | (many diffs) | |
| 7 | human | 2653 | GCSH | glycine cleavage system pro... | NR_033249.2 | 29.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 585
- ORF length:
- 519
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gctgcgagtg gtgcggagcg tgcgggccct gctctgcacc ctgcgcgcgg 121 tcccgttacc cgccgcgccc tgcccgccga ggccctggca gctgggggtg ggcgccgtcc 181 gtacgctgcg cactggaccc gctctgctct cggtgcgtaa attcacagag aaacacgaat 241 gggtaacaaC AGAAAATGGC ATTGGAACAG TGGGAATCAG CAATTTTGCA CAGGAAGCGT 301 TGGGAGATGT TGTTTATTGT AGTCTCCCTG AAGTTGGGAC AAAATTGAAC AAACAAGATG 361 AGTTTGGTGC TTTGGAAAGT GTGAAAGCTG CTAGTGAACT CTATTCTCCT TTATCAGGAG 421 AAGTAACTGA AATTAATGAA GCTCTTGCAG AAAATCCAGG ACTTGTAAAC AAATCTTGTT 481 ATGAAGATGG TTGGCTGATC AAGATGACAC TGAGTAACCC TTCAGAACTA GATGAACTTA 541 TGAGTGAAGA AGCATATGAG AAATACATAA AATCTATTGA GGAGTGCCCA ACTTTCTTGT 601 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 661 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 721 GAAAGGACGA TCATGCTTAG TCCCCTTTCG GGCAACGCGT TAAGTCgaca atcaacctct 781 ggattacaaa atttgtgaaa gatt