Construct: ORF TRCN0000471721
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016379.1_s317c1
- Derived from:
- ccsbBroadEn_15684
- DNA Barcode:
- TGCCTTCTTGTCGTTCTTTACTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARHGAP44 (9912)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471721
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9912 | ARHGAP44 | Rho GTPase activating prote... | NM_001321168.2 | 19.1% | 19% | 1_1377del;1700C>T |
| 2 | human | 9912 | ARHGAP44 | Rho GTPase activating prote... | XM_005256891.2 | 14.7% | 14.4% | 1_1755del;2078_2080delATC;2085_2214delinsG |
| 3 | human | 9912 | ARHGAP44 | Rho GTPase activating prote... | NM_001321164.2 | 14.1% | 14% | 1_1977del;2300C>T |
| 4 | human | 9912 | ARHGAP44 | Rho GTPase activating prote... | NM_001321167.2 | 14% | 13.9% | 1_1995del;2318C>T |
| 5 | human | 9912 | ARHGAP44 | Rho GTPase activating prote... | NM_001321166.2 | 13.3% | 13.1% | 1_1977del;2300_2302delATC;2307_2436delinsG |
| 6 | human | 9912 | ARHGAP44 | Rho GTPase activating prote... | NM_014859.6 | 13.2% | 13% | 1_1995del;2318_2320delATC;2325_2454delinsG |
| 7 | human | 9912 | ARHGAP44 | Rho GTPase activating prote... | NR_135569.2 | 8.4% | 1_1920del;2243_2245delATC;2250_3854delinsG | |
| 8 | mouse | 216831 | Arhgap44 | Rho GTPase activating prote... | XM_017314452.1 | 13.6% | 14% | (many diffs) |
| 9 | mouse | 216831 | Arhgap44 | Rho GTPase activating prote... | XM_006532874.3 | 13% | 13.3% | (many diffs) |
| 10 | mouse | 216831 | Arhgap44 | Rho GTPase activating prote... | XM_006532873.3 | 12.8% | 13.1% | (many diffs) |
| 11 | mouse | 216831 | Arhgap44 | Rho GTPase activating prote... | NM_175003.3 | 12.2% | 12.6% | (many diffs) |
| 12 | mouse | 216831 | Arhgap44 | Rho GTPase activating prote... | XM_006532872.3 | 12.2% | 12.5% | (many diffs) |
| 13 | mouse | 216831 | Arhgap44 | Rho GTPase activating prote... | NM_001099288.1 | 11.6% | 11.8% | (many diffs) |
| 14 | mouse | 216831 | Arhgap44 | Rho GTPase activating prote... | XM_006532871.3 | 11.5% | 11.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 417
- ORF length:
- 327
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCAGACCA GTCCGCTGGC CAGCCGTCCC 121 CAGTCAGCCT GTCCCCCACC CCGCCCAGCA CCCCGTCACC CTATGGACTG AGCTACCCTC 181 AGGGGTACTC CTTGGCCTCG GGCCAGCTCT CCCCAGCTGC AGCTCCTCCC CTGGCCTCTC 241 CTTCTGTCTT TACAAGCACT TTGAGCAAAT CGCGGCCCAC TCCTAAGCCG CGACAGAGAC 301 CTACTCTGCC GCCTCCTCAG CCTCCCACAG TAAACCTCTC GGCCTCTAGT CCACAGTCCA 361 CGGAGGCCCC CATGCTAGAT GGCATGTCCC CTGGGGAAAG CATGTCTACA GTTGTGTGCC 421 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 481 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 541 ATATATCTTG TGGAAAGGAC GATGCCTTCT TGTCGTTCTT TACTTTACGC GTTAAGTCga 601 caatcaacct ctggattaca aaatttgtga aagatt