Construct: ORF TRCN0000471741
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003888.1_s317c1
- Derived from:
- ccsbBroadEn_06482
- DNA Barcode:
- CTCCGCTTACACCTAAGTAACTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNJ13 (3769)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471741
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3769 | KCNJ13 | potassium inwardly rectifyi... | NM_002242.4 | 99.7% | 99.1% | 524C>T;869C>A;925G>T |
| 2 | human | 3769 | KCNJ13 | potassium inwardly rectifyi... | NM_001172417.1 | 77.5% | 76.9% | (many diffs) |
| 3 | human | 3769 | KCNJ13 | potassium inwardly rectifyi... | NM_001172416.1 | 26.1% | 22.2% | 223_224ins236;282_283ins562 |
| 4 | mouse | 100040591 | Kcnj13 | potassium inwardly-rectifyi... | NM_001110227.2 | 89.9% | 92.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1146
- ORF length:
- 1080
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cagcagtaat tgcaaagtta ttgctcctct cctaagtcaa agataccgga 121 ggatggtcac caaggatggc cacagcacac ttcaaatgga tggcgctcaa agaggtcttg 181 catatcttcg agatgcttgg ggaatcctaa tggacatgcg ctggcgttgg atgatgttgg 241 tcttttctgc ttcttttgtt gtccactggc ttgtctttgc agtgctctgg tatgttctgg 301 ctgagatgaa tggtgatctg gaactagatc atgatgcccc acctgaaaac cacactatct 361 gtgtcaagta tatcaccagt ttcacagctg cattctcctt ctccctggag acacaactca 421 caattggtta tggtaccatg ttccccagtg gtgactgtcc aagtgcaatc gccttacttg 481 ccatacaaat gctcctaggc ctcatgctag aggcttttat cacaggtgct tttgtggcga 541 agattgcccg gccaaaaaat cgagcttttt caattcgctt tactgacata gcagtagtag 601 ctcacatgga tggcaaacct aatcttatct tccaagtggC CAACACCCGA CCTAGCCCTC 661 TAACCAGTGT CCGGGTCTCA GCTGTACTCT ATCAGGAAAG AGAAAATGGC AAACTCTACC 721 AGACCAGTGT GGATTTCCAC CTTGATGGCA TCAGTTCTGA CGAATGTCCA TTCTTCATCT 781 TTCCACTAAC GTACTATCAC TCCATTACAC CATCAAGTCC TCTGGCTACT CTGCTCCAGC 841 ATGAAAATCC TTCTCACTTT GAATTAGTTG TATTCCTTTC AGCAATGCAG GAGGGCACTG 901 GAGAAATATG CCAAAGGAGG ACATCCTACC TACAGTCTGA AATCATGTTA CATCACTGTT 961 TTGCATCTCT GTTGACCCGA GGTTCCAAAT GTGAATATCA AATCAAGATG GAGAATTTTG 1021 ACAAGACTGT CCCTGAATTT CCAACTCCTC TGGTTTCTAA AAGCCCAAAC AGGACTGACC 1081 TGGATATCCA CATCAATGGA CAAAGCATTG ACAATTTTCA GATCTCTGAA ACAGGACTGA 1141 CAGAATACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1201 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1261 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACTCCGCTTA CACCTAAGTA ACTTAACGCG 1321 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt