Construct: ORF TRCN0000471757
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007312.1_s317c1
- Derived from:
- ccsbBroadEn_10236
- DNA Barcode:
- ATGGTTAGTAAGAGCCTCTAAGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- INGX (27160)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471757
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3621 | ING1 | inhibitor of growth family ... | NM_198218.2 | 17.6% | 14.2% | (many diffs) |
| 2 | human | 27160 | INGX | inhibitor of growth family,... | NR_002226.3 | 16.3% | 1_202del;329_769del | |
| 3 | human | 3621 | ING1 | inhibitor of growth family ... | NM_198217.2 | 15.7% | 12.7% | (many diffs) |
| 4 | human | 3621 | ING1 | inhibitor of growth family ... | NM_001267728.1 | 14.1% | 11.4% | (many diffs) |
| 5 | human | 3621 | ING1 | inhibitor of growth family ... | NM_198219.3 | 13.2% | 10.7% | (many diffs) |
| 6 | human | 3621 | ING1 | inhibitor of growth family ... | NM_005537.5 | 8.7% | 7.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 323
- ORF end:
- 449
- ORF length:
- 126
- Sequence:
-
1 TCAGCCGTCG CTTCATGTGA CTCCACGGAG TACCGGGCGC CGTCCAGGCA CCTCGATTAG 61 TTCTCGAGCT TTTGGAGTAC GTCGTCTTTA GGTTGGGGGG AGGGGTTTTA TGCGATGGAG 121 TTTCCCCACA CTGAGTGGGT GGAGACTGAA GTTAGGCCAG CTTGGCACTT GATGTAATTC 181 TCCTTGGAAT TTGCCCTTTT TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG 241 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT GATCAACAAG 301 TTTGTACAAA AAAGTTGGCA CCATGATCCG CTGCGACAAC GAATGCCCCA TCGAGTGGTT 361 CCGCTTCTCG TGTGTGAGTC TCAACCATAA ACCAAAGCGC AAGTGGTACT GTTCCAGATG 421 CCGGGGAAAG AACGATGGGC AAAGCCCTTT GCCAACTTTC TTGTACAAAG TGGTTGATAT 481 CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC 541 GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATGGTT 601 AGTAAGAGCC TCTAAGAAAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt 661 gaaagatt