Construct: ORF TRCN0000471758
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007753.1_s317c1
- Derived from:
- ccsbBroadEn_08197
- DNA Barcode:
- CCATCACGCGACAACTATGAAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GOLT1B (51026)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471758
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51026 | GOLT1B | golgi transport 1B | NM_016072.5 | 99.7% | 99.2% | 407A>C |
| 2 | human | 51026 | GOLT1B | golgi transport 1B | XM_024448999.1 | 58.3% | 58% | 1_294del;701A>C |
| 3 | mouse | 66964 | Golt1b | golgi transport 1B | NM_025872.4 | 89.1% | 97.8% | (many diffs) |
| 4 | mouse | 66964 | Golt1b | golgi transport 1B | XM_006506981.3 | 82.1% | 89.8% | (many diffs) |
| 5 | mouse | 66964 | Golt1b | golgi transport 1B | XM_011241596.2 | 74.1% | 78.7% | (many diffs) |
| 6 | mouse | 66964 | Golt1b | golgi transport 1B | XM_011241597.2 | 74.1% | 78.7% | (many diffs) |
| 7 | mouse | 66964 | Golt1b | golgi transport 1B | XR_869553.1 | 51.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 480
- ORF length:
- 414
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttG TACAAAAAAG 61 TTGGCATGAT CTCCTTAACG GACACGCAGA AAATTGGAAT GGGATTAACA GGATTTGGAG 121 TGTTTTTCCT GTTCTTTGGA ATGATTCTCT TTTTTGACAA AGCACTACTG GCTATTGGAA 181 ATGTTTTATT TGTAGCCGGC TTGGCTTTTG TAATTGGTTT AGAAAGAACA TTCAGATTCT 241 TCTTCCAAAA ACATAAAATG AAAGCTACAG GTTTTTTTCT GGGTGGTGTA TTTGTAGTCC 301 TTATTGGTTG GCCTTTGATA GGCATGATCT TCGAAATTTA TGGATTTTTT CTCTTGTTCA 361 GGGGCTTCTT TCCTGTCGTT GTTGGCTTTA TTAGAAGAGT GCCAGTCCTT GGATCCCTCC 421 TAAATTTACC TGGAATTAGA TCATTTGTAG ATAAAGTTGG AGAAAGCAAC ACTATGGTAT 481 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 541 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 601 TTTATATATC TTGTGGAAAG GACGACCATC ACGCGACAAC TATGAAAATA CGCGTTAAGT 661 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt