Transcript: Mouse XR_869553.1

PREDICTED: Mus musculus golgi transport 1B (Golt1b), transcript variant X3, misc_RNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Golt1b (66964)
Length:
703
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_869553.1
NBCI Gene record:
Golt1b (66964)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XR_869553.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000133954 CCTGTTCTTTGGAATGATTCT pLKO.1 196 3UTR 100% 4.950 3.960 N GOLT1B n/a
2 TRCN0000343520 CCTGTTCTTTGGAATGATTCT pLKO_005 196 3UTR 100% 4.950 3.960 N GOLT1B n/a
3 TRCN0000126155 GCCTCTGATAGGCATGATCTT pLKO.1 379 3UTR 100% 4.950 3.960 N Golt1b n/a
4 TRCN0000126156 GTTGGCTTTATCAGAAGAGTA pLKO.1 449 3UTR 100% 4.950 3.960 N Golt1b n/a
5 TRCN0000305017 TCCTCGGATCCCTCCTAAATC pLKO_005 474 3UTR 100% 13.200 9.240 N Golt1b n/a
6 TRCN0000134476 GATAGGCATGATCTTCGAAAT pLKO.1 385 3UTR 100% 10.800 7.560 N GOLT1B n/a
7 TRCN0000343521 GATAGGCATGATCTTCGAAAT pLKO_005 385 3UTR 100% 10.800 7.560 N GOLT1B n/a
8 TRCN0000126157 CGAGAGAACGTTCAGATTCTT pLKO.1 289 3UTR 100% 5.625 3.938 N Golt1b n/a
9 TRCN0000308351 CGAGAGAACGTTCAGATTCTT pLKO_005 289 3UTR 100% 5.625 3.938 N Golt1b n/a
10 TRCN0000159888 GTTCTTTGGAATGATTCTCTT pLKO.1 199 3UTR 100% 4.950 3.465 N GOLT1B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_869553.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08197 pDONR223 100% 51.2% None (many diffs) n/a
2 ccsbBroad304_08197 pLX_304 0% 51.2% V5 (many diffs) n/a
3 TRCN0000471758 CCATCACGCGACAACTATGAAAAT pLX_317 100% 51.2% V5 (many diffs) n/a
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