Transcript: Human NM_016072.5

Homo sapiens golgi transport 1B (GOLT1B), mRNA.

Source:
NCBI, updated 2019-05-02
Taxon:
Homo sapiens (human)
Gene:
GOLT1B (51026)
Length:
3253
CDS:
136..552

Additional Resources:

NCBI RefSeq record:
NM_016072.5
NBCI Gene record:
GOLT1B (51026)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_016072.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000161911 GACAAAGCACTACTGGCTATT pLKO.1 226 CDS 100% 10.800 15.120 N GOLT1B n/a
2 TRCN0000158960 GCAAGAATAGTATCTGCTAAT pLKO.1 2384 3UTR 100% 10.800 15.120 N GOLT1B n/a
3 TRCN0000136775 CCGGAAAGTCTCATACTTCTT pLKO.1 1236 3UTR 100% 4.950 6.930 N GOLT1B n/a
4 TRCN0000311218 GAAAGCAACAATATGGTATAA pLKO_005 532 CDS 100% 13.200 10.560 N Golt1b n/a
5 TRCN0000162456 CCTTATTGGTTGGCCTTTGAT pLKO.1 369 CDS 100% 5.625 4.500 N GOLT1B n/a
6 TRCN0000343569 CCTTATTGGTTGGCCTTTGAT pLKO_005 369 CDS 100% 5.625 4.500 N GOLT1B n/a
7 TRCN0000159888 GTTCTTTGGAATGATTCTCTT pLKO.1 201 CDS 100% 4.950 3.960 N GOLT1B n/a
8 TRCN0000134476 GATAGGCATGATCTTCGAAAT pLKO.1 387 CDS 100% 10.800 7.560 N GOLT1B n/a
9 TRCN0000343521 GATAGGCATGATCTTCGAAAT pLKO_005 387 CDS 100% 10.800 7.560 N GOLT1B n/a
10 TRCN0000159195 GCACTATACATCCTATTGTTT pLKO.1 2704 3UTR 100% 5.625 3.938 N GOLT1B n/a
11 TRCN0000343570 GCACTATACATCCTATTGTTT pLKO_005 2704 3UTR 100% 5.625 3.938 N GOLT1B n/a
12 TRCN0000160906 GCCTTTGATAGGCATGATCTT pLKO.1 381 CDS 100% 4.950 3.465 N GOLT1B n/a
13 TRCN0000133954 CCTGTTCTTTGGAATGATTCT pLKO.1 198 CDS 100% 4.950 2.970 N GOLT1B n/a
14 TRCN0000343520 CCTGTTCTTTGGAATGATTCT pLKO_005 198 CDS 100% 4.950 2.970 N GOLT1B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_016072.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08197 pDONR223 100% 99.7% 99.2% None 407A>C n/a
2 ccsbBroad304_08197 pLX_304 0% 99.7% 99.2% V5 407A>C n/a
3 TRCN0000471758 CCATCACGCGACAACTATGAAAAT pLX_317 100% 99.7% 99.2% V5 407A>C n/a
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