Construct: ORF TRCN0000471759
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011520.1_s317c1
- Derived from:
- ccsbBroadEn_08519
- DNA Barcode:
- AAACGTTTCAACTTCTCTCCTGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNLS (55328)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471759
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55328 | RNLS | renalase, FAD dependent ami... | NM_001031709.3 | 99.8% | 99.4% | 4G>T;111G>C |
| 2 | human | 55328 | RNLS | renalase, FAD dependent ami... | NM_018363.3 | 89.4% | 85.3% | (many diffs) |
| 3 | human | 55328 | RNLS | renalase, FAD dependent ami... | XM_011539924.3 | 89.4% | 85.3% | (many diffs) |
| 4 | human | 55328 | RNLS | renalase, FAD dependent ami... | XM_017016380.2 | 89.4% | 85.3% | (many diffs) |
| 5 | human | 55328 | RNLS | renalase, FAD dependent ami... | XM_005269946.2 | 88% | 84.8% | (many diffs) |
| 6 | human | 55328 | RNLS | renalase, FAD dependent ami... | XM_011539927.3 | 86.1% | 84.1% | (many diffs) |
| 7 | human | 55328 | RNLS | renalase, FAD dependent ami... | XM_017016381.2 | 85.1% | 84.7% | 4G>T;111G>C;876_877ins150 |
| 8 | human | 55328 | RNLS | renalase, FAD dependent ami... | XM_005269947.2 | 77.2% | 64.4% | (many diffs) |
| 9 | human | 55328 | RNLS | renalase, FAD dependent ami... | XM_005269948.3 | 75.5% | 75.1% | 4G>T;111G>C;114_115ins249 |
| 10 | human | 55328 | RNLS | renalase, FAD dependent ami... | XM_005269949.5 | 67.9% | 67.5% | (many diffs) |
| 11 | human | 55328 | RNLS | renalase, FAD dependent ami... | XM_005269950.4 | 65.3% | 61.3% | (many diffs) |
| 12 | human | 55328 | RNLS | renalase, FAD dependent ami... | XM_017016382.2 | 65.3% | 61.3% | (many diffs) |
| 13 | human | 55328 | RNLS | renalase, FAD dependent ami... | XM_024448063.1 | 63.8% | 60.6% | (many diffs) |
| 14 | human | 55328 | RNLS | renalase, FAD dependent ami... | XM_017016383.2 | 55.8% | 48% | (many diffs) |
| 15 | human | 55328 | RNLS | renalase, FAD dependent ami... | XM_017016384.2 | 53% | 41.5% | (many diffs) |
| 16 | human | 55328 | RNLS | renalase, FAD dependent ami... | XM_017016385.1 | 52% | 48.5% | (many diffs) |
| 17 | human | 55328 | RNLS | renalase, FAD dependent ami... | XR_001747122.2 | 17.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1092
- ORF length:
- 1026
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gcaggtgctg atcgtgggcg ccgggatgac aggaagcttg tgcgctgcgc 121 tgctgaggag gcagacgtcc ggtcccttgt accttgctgt gtgggacaag gctgacgact 181 cagggggaag aatgactaca gcctgcagtc ctcataatcc tcagtgcaca gctgacttgg 241 gtgctcagta catcacctgc actcctcatt atgccaaaaa acaccaacgt ttttatgatg 301 aactgttagc ctatggcgtt ttgaggcctc taagctcgcc tattgaagga atggtgatga 361 aagaaggaga ctgtaacttt gtggcacctc aaggaatttc ttcaattatt aagcattact 421 tgaaagaatc aggtgcagaa gtctacttca gacatcgtgt gacacagatc aacctaagag 481 atgacaaatg ggaagtatcc aaacaaacag gctcccctga gcagtttgat cttattgttc 541 tcacaatgcc agttcctgag attctgcagc ttcaaggtga catcaccacc ttaattagtg 601 aatgccaaag gcagcaactg gaggctgtga gctactcctc tcgatatgct ctgggCCTCT 661 TTTATGAAGC TGGTACGAAG ATTGATGTCC CTTGGGCTGG GCAGTACATC ACCAGTAATC 721 CCTGCATACG CTTCGTCTCC ATTGATAATA AGAAGCGCAA TATAGAGTCA TCAGAAATTG 781 GGCCTTCCCT CGTGATTCAC ACCACTGTCC CATTTGGAGT TACATACTTG GAACACAGCA 841 TTGAGGATGT GCAAGAGTTA GTCTTCCAGC AGCTGGAAAA CATTTTGCCG GGTTTGCCTC 901 AGCCAATTGC TACCAAATGC CAAAAATGGA GACATTCACA GGTTACAAAT GCTGCTGCCA 961 ACTGTCCTGG CCAAATGACT CTGCATCACA AACCTTTCCT TGCATGTGGA GGGGATGGAT 1021 TTACTCAGTC CAACTTTGAT GGCTGCATCA CTTCTGCCCT ATGTGTTCTG GAAGCTTTAA 1081 AGAATTATAT TTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1141 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1201 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAAA CGTTTCAACT TCTCTCCTGC 1261 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t