Construct: ORF TRCN0000471766

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012002.1_s317c1
Derived from:
ccsbBroadEn_06156
DNA Barcode:
CGTTACGCACTCAAGTCTTTGTGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ELF2 (1998)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471766
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1998 ELF2 E74 like ETS transcription ... NM_006874.4 99.9% 99.8% 1599A>T
2 human 1998 ELF2 E74 like ETS transcription ... NM_001276458.2 97.6% 97.5% 171_172ins36;1563A>T
3 human 1998 ELF2 E74 like ETS transcription ... NM_001276457.1 94.4% 94.3% 341_342ins87;1512A>T
4 human 1998 ELF2 E74 like ETS transcription ... NM_001276459.2 92.2% 92.1% 171_172ins36;305_306ins87;1476A>T
5 human 1998 ELF2 E74 like ETS transcription ... XM_011531711.2 91.6% 91.5% 0_1ins132;1467A>T
6 human 1998 ELF2 E74 like ETS transcription ... XM_024453918.1 91.6% 91.5% 0_1ins132;1467A>T
7 human 1998 ELF2 E74 like ETS transcription ... XM_024453919.1 91.6% 91.5% 0_1ins132;1467A>T
8 human 1998 ELF2 E74 like ETS transcription ... XM_024453920.1 91.6% 91.5% 0_1ins132;1467A>T
9 human 1998 ELF2 E74 like ETS transcription ... NM_001371337.1 89.4% 89.3% 0_1ins132;39_40ins36;1431A>T
10 human 1998 ELF2 E74 like ETS transcription ... XM_024453922.1 89.4% 89.3% 0_1ins132;39_40ins36;1431A>T
11 human 1998 ELF2 E74 like ETS transcription ... NM_001331036.2 88% 86.7% (many diffs)
12 human 1998 ELF2 E74 like ETS transcription ... NM_001371338.1 86.2% 86.1% 0_1ins132;209_210ins87;1380A>T
13 human 1998 ELF2 E74 like ETS transcription ... XM_024453924.1 86.2% 86.1% 0_1ins132;209_210ins87;1380A>T
14 human 1998 ELF2 E74 like ETS transcription ... NM_001371324.1 86% 84.6% (many diffs)
15 human 1998 ELF2 E74 like ETS transcription ... NM_201999.3 86% 84.6% (many diffs)
16 human 1998 ELF2 E74 like ETS transcription ... NM_001371339.1 83.9% 83.8% (many diffs)
17 human 1998 ELF2 E74 like ETS transcription ... XM_024453925.1 83.9% 83.8% (many diffs)
18 human 1998 ELF2 E74 like ETS transcription ... XM_024453926.1 83.9% 83.8% (many diffs)
19 human 1998 ELF2 E74 like ETS transcription ... NM_001371336.1 83.1% 81.8% (many diffs)
20 human 1998 ELF2 E74 like ETS transcription ... XM_005262805.2 81.1% 79.7% (many diffs)
21 human 1998 ELF2 E74 like ETS transcription ... XM_011531714.2 62.7% 62.6% 0_1ins594;1005A>T
22 mouse 69257 Elf2 E74-like factor 2 NM_001291063.1 89.1% 93.2% (many diffs)
23 mouse 69257 Elf2 E74-like factor 2 NM_001291062.1 87% 91.2% (many diffs)
24 mouse 69257 Elf2 E74-like factor 2 XM_006502031.4 85.5% 89.5% (many diffs)
25 mouse 69257 Elf2 E74-like factor 2 NM_001356358.1 83.4% 87.4% (many diffs)
26 mouse 69257 Elf2 E74-like factor 2 XM_017319714.2 81.1% 85% (many diffs)
27 mouse 69257 Elf2 E74-like factor 2 XM_030252804.1 79% 82.9% (many diffs)
28 mouse 69257 Elf2 E74-like factor 2 XM_030252805.1 79% 82.9% (many diffs)
29 mouse 69257 Elf2 E74-like factor 2 NM_023502.2 78.3% 79.5% (many diffs)
30 mouse 69257 Elf2 E74-like factor 2 NM_001291059.1 76.5% 77.7% (many diffs)
31 mouse 69257 Elf2 E74-like factor 2 XM_006502034.4 75.6% 79.4% (many diffs)
32 mouse 69257 Elf2 E74-like factor 2 XM_030252806.1 75.6% 79.4% (many diffs)
33 mouse 69257 Elf2 E74-like factor 2 XM_030252807.1 75.6% 79.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1665
ORF length:
1599
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc gacgtctctg catgagggac ccacgaacca gctggatctg ctcatccggg
121ccgtggaagc atcagttcac agcagtaatg cacactgtac agataagaca attgaagctg
181ctgaagccct gcttcatatg gaatctccta cctgcttgag ggattcaaga agtcctgtgg
241aagtgtttgt tcctccttgt gtatcaactc cagaattcat ccatgctgct atgaggccag
301atgtcattac agaaactgta gtggaggtgt caactgaaga gtctgaaccc atggatacct
361ctcctattcc aacatcacca gatagccatg aaccaatgaa aaagaaaaaa gttggccgta
421aaccaaagac ccagcaatca ccaatttcca atgggtctcc tgagttaggt ataaagaaga
481aaccaagaga aggaaaagga aacacaacct atttgtggga gtttctttta gatctacttc
541aagataaaaa tacttgtccc aggtatatta aatggactca gagagaaaaa ggcatattca
601agctggtgga ttcaaaggct gtctctaagc tttggggaaa gcataagaac aaaccagaca
661tgaactatga aaccatggga cgagctttga gatactacta ccaaagggga attcttgcaa
721aggttgaagg acagaggctt gtatatcagt tcaaggatat gccgaaaaac atagtggtca
781tagatgatga caaaagtgaa acctgtaatg aagatttagc aggaactact gatgaaaaat
841cattagaacg agtgtcactg tctgcagaaa gtctcctgaa agcagcatcc tctgttcgca
901gtggaaaaaa ttcatcccct ataaactgct ccagagcaga gaagggtgta gctagagttg
961tgaatatcac ttcccctggg cacgatgctt catccaggtc tcctactacc actgcatctg
1021tgtcagcaac agcagctcca aggacagttc gtgtggcaat gcaggtacct gttgtaatga
1081catcattggg tcagaaaatt tcaactgtgg cagttcagtc agttaatgca ggtgcaccat
1141taataaccag cactagtcca acaacagcga cctctccaaa ggtagtcatt cagacaatcc
1201cTACTGTGAT GCCAGCTTCT ACTGAAAATG GAGACAAAAT CACCATGCAG CCTGCCAAAA
1261TTATTACCAT CCCAGCTACA CAGCTTGCAC AGTGTCAACT GCAGACAAAG TCAAATCTGA
1321CTGGATCAGG AAGCATTAAC ATTGTTGGAA CCCCATTGGC TGTGAGAGCA CTTACCCCTG
1381TTTCAATAGC CCATGGTACA CCTGTAATGA GACTATCAAT GCCTACTCAG CAGGCATCTG
1441GCCAGACTCC TCCTCGAGTT ATCAGTGCAG TCATAAAGGG GCCAGAGGTT AAATCGGAAG
1501CAGTGGCAAA AAAGCAAGAA CATGATGTGA AAACTTTGCA GCTAGTAGAA GAAAAACCAG
1561CAGATGGAAA TAAGACAGTG ACCCACGTAG TGGTTGTCAG TGCGCCTTCA GCTATTGCCC
1621TTCCTGTAAC TATGAAAACA GAAGGACTAG TGACATGTGA GAATTACCCA ACTTTCTTGT
1681ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1741AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1801GAAAGGACGA CGTTACGCAC TCAAGTCTTT GTGCACGCGT TAAGTCgaca atcaacctct
1861ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)