Construct: ORF TRCN0000471788
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007000.4_s317c1
- Derived from:
- ccsbBroadEn_15133
- DNA Barcode:
- TTTAATATTGACAGGAGCATGTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C17orf75 (64149)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471788
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64149 | C17orf75 | chromosome 17 open reading ... | NM_022344.4 | 100% | 100% | |
| 2 | human | 64149 | C17orf75 | chromosome 17 open reading ... | XM_005258022.4 | 97.9% | 97.9% | 0_1ins24 |
| 3 | human | 64149 | C17orf75 | chromosome 17 open reading ... | XM_017024940.2 | 54.7% | 54.7% | 0_1ins537 |
| 4 | human | 64149 | C17orf75 | chromosome 17 open reading ... | XM_017024941.2 | 54.7% | 54.7% | 0_1ins537 |
| 5 | mouse | 70591 | 5730455P16Rik | RIKEN cDNA 5730455P16 gene | NM_027472.3 | 88.6% | 89.1% | (many diffs) |
| 6 | mouse | 70591 | 5730455P16Rik | RIKEN cDNA 5730455P16 gene | XM_006534198.2 | 57.1% | 58% | (many diffs) |
| 7 | mouse | 70591 | 5730455P16Rik | RIKEN cDNA 5730455P16 gene | XM_006534199.3 | 57.1% | 58% | (many diffs) |
| 8 | mouse | 70591 | 5730455P16Rik | RIKEN cDNA 5730455P16 gene | XM_006534197.3 | 56.9% | 50.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1254
- ORF length:
- 1188
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct cccctctttg caggagtcga tggatggaga tgaaaaggaa ctagagagca 121 gcgaagaggg aggctcagcc gaggagcgga gactcgagcc gccgtccagc agccactact 181 gtctttacag ctatcgcgga agcagattgg cacagcaacg aggggacagt gaggacggaa 241 gcccaagtgg cacaaatgca gaaactccct ctggtgatga tttcagcctc tccttggcag 301 atactaatct accatccgaa gtggagccag agctgcgcag tttcattgct aagcgtcttt 361 caagaggtgc agtctttgaa gggctgggta atgttgcatc tgtggagcta aaaattccag 421 gttaccgagt tggttgttat tactgccttt tccaaaatga aaaactgctt cctgaaacag 481 taacgataga ctctgaacgt aacccttcag aatatgtggt ctgtttttta ggagggtctg 541 aaaaaggact tgagcttttc aggcttgaat tggacaagta cattcaaggg ctgaaaaata 601 acatgaattg tgaggcaagg ggcctggaga gtcacataaa atcctatctg agcagctggt 661 ttgaggatgt tgtatgccca atccaaaggg ttgttcttct ctttcaggaa aagcttacct 721 ttctgctaca tgctgctttg agttacactc ctgttgaagt taaagaatca gatGAAAAAA 781 CAAAGAGAGA CATTAACAGG TTTCTGAGTG TGGCCAGTCT TCAAGGACTT ATTCATGAAG 841 GCACCATGAC TTCTTTGTGC ATGGCCATGA CAGAGGAGCA GCATAAGTCT GTGGTCATCG 901 ATTGCAGCAG CTCCCAGCCT CAGTTCTGCA ATGCAGGAAG TAACCGGTTT TGTGAGGATT 961 GGATGCAAGC TTTTTTAAAT GGTGCCAAAG GAGGTAACCC TTTTCTTTTC CGACAAGTAC 1021 TGGAGAACTT TAAACTAAAG GCCATACAAG ACACAAACAA TTTGAAGAGA TTTATCCGAC 1081 AGGCAGAAAT GAATCATTAT GCTTTGTTTA AATGTTACAT GTTCCTAAAG AACTGTGGTA 1141 GTGGAGATAT ACTTTTGAAG ATTGTTAAAG TGGAACATGA AGAAATGCCT GAAGCCAAAA 1201 ATGTGATAGC TGTCCTTGAA GAATTCATGA AAGAAGCTCT TGACCAAAGT TTTTGCCCAA 1261 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1321 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1381 TATCTTGTGG AAAGGACGAT TTAATATTGA CAGGAGCATG TCTACGCGTT AAGTCgacaa 1441 tcaacctctg gattacaaaa tttgtgaaag att