Transcript: Human XM_005258022.4

PREDICTED: Homo sapiens chromosome 17 open reading frame 75 (C17orf75), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
C17orf75 (64149)
Length:
1454
CDS:
115..1281

Additional Resources:

NCBI RefSeq record:
XM_005258022.4
NBCI Gene record:
C17orf75 (64149)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001487133 TAGATTGGCACAGCAACGAG pXPR_003 GGG 130 11% 3 0.4093 C17orf75 C17orf75 78079
2 BRDN0001146368 TACTAATCTACCATCCGAAG pXPR_003 TGG 229 20% 4 0.104 C17orf75 C17orf75 78078
3 BRDN0001145175 TGTTGTATGCCCAATCCAAA pXPR_003 GGG 595 51% 7 -0.0660 C17orf75 C17orf75 78077
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005258022.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000195492 CGATAGACTCTGAACGTAACC pLKO.1 509 CDS 100% 4.050 3.240 N C17orf75 n/a
2 TRCN0000037674 GCCAGTCTTCAAGGACTTATT pLKO.1 838 CDS 100% 13.200 9.240 N C17orf75 n/a
3 TRCN0000196906 GTTACCGAGTTGGTTGTTATT pLKO.1 446 CDS 100% 13.200 9.240 N C17orf75 n/a
4 TRCN0000196990 GAAGGCACCATGACTTCTTTG pLKO.1 862 CDS 100% 10.800 7.560 N C17orf75 n/a
5 TRCN0000037678 GCCATACAAGACACAAACAAT pLKO.1 1066 CDS 100% 5.625 3.938 N C17orf75 n/a
6 TRCN0000194998 GATTTGAAACTCAATCCAGAT pLKO.1 1379 3UTR 100% 4.050 2.835 N C17orf75 n/a
7 TRCN0000037677 GCTTTGAGTTACACTCCTGTT pLKO.1 760 CDS 100% 4.050 2.835 N C17orf75 n/a
8 TRCN0000037676 GCTTCCTGAAACAGTAACGAT pLKO.1 492 CDS 100% 3.000 2.100 N C17orf75 n/a
9 TRCN0000037675 GCAGCATAAGTCTGTGGTCAT pLKO.1 903 CDS 100% 4.050 2.430 N C17orf75 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005258022.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03932 pDONR223 100% 97.9% 97.9% None 0_1ins24 n/a
2 TRCN0000478392 AACGCGCACTTCGTACGTGTCAAG pLX_317 28.5% 97.9% 97.9% V5 0_1ins24 n/a
3 ccsbBroadEn_15133 pDONR223 0% 97.9% 97.9% None 0_1ins24 n/a
4 ccsbBroad304_15133 pLX_304 0% 97.9% 97.9% V5 0_1ins24 n/a
5 TRCN0000471788 TTTAATATTGACAGGAGCATGTCT pLX_317 40.6% 97.9% 97.9% V5 0_1ins24 n/a
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