Transcript: Mouse NM_027472.3

Mus musculus RIKEN cDNA 5730455P16 gene (5730455P16Rik), mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
5730455P16Rik (70591)
Length:
4536
CDS:
35..1216

Additional Resources:

NCBI RefSeq record:
NM_027472.3
NBCI Gene record:
5730455P16Rik (70591)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_027472.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000196906 GTTACCGAGTTGGTTGTTATT pLKO.1 390 CDS 100% 13.200 18.480 N C17orf75 n/a
2 TRCN0000079183 CCGTAGTGTTTAGCAGGATTT pLKO.1 1511 3UTR 100% 10.800 15.120 N 5730455P16Rik n/a
3 TRCN0000079186 CCGGAACTTCGCAGTTTCATT pLKO.1 296 CDS 100% 5.625 7.875 N 5730455P16Rik n/a
4 TRCN0000362952 GGACGTTCAGGTGGCATAAAT pLKO_005 206 CDS 100% 15.000 10.500 N 5730455P16Rik n/a
5 TRCN0000363019 GCTTGAACTGGACAAGTATAT pLKO_005 532 CDS 100% 13.200 9.240 N 5730455P16Rik n/a
6 TRCN0000079187 GATGTAAAGTCCTATCTGAAT pLKO.1 602 CDS 100% 4.950 3.465 N 5730455P16Rik n/a
7 TRCN0000363017 CCAGTCTCCAGGGACTTATTC pLKO_005 783 CDS 100% 13.200 7.920 N 5730455P16Rik n/a
8 TRCN0000079185 CGCTCTGTTTAAATGTTACAT pLKO.1 1069 CDS 100% 5.625 3.375 N 5730455P16Rik n/a
9 TRCN0000079184 GCTTCTTAGGAGGCTCTGAAA pLKO.1 492 CDS 100% 4.950 2.970 N 5730455P16Rik n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_027472.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03932 pDONR223 100% 88.6% 89.1% None (many diffs) n/a
2 TRCN0000478392 AACGCGCACTTCGTACGTGTCAAG pLX_317 28.5% 88.6% 89.1% V5 (many diffs) n/a
3 ccsbBroadEn_15133 pDONR223 0% 88.6% 89.1% None (many diffs) n/a
4 ccsbBroad304_15133 pLX_304 0% 88.6% 89.1% V5 (many diffs) n/a
5 TRCN0000471788 TTTAATATTGACAGGAGCATGTCT pLX_317 40.6% 88.6% 89.1% V5 (many diffs) n/a
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