Construct: ORF TRCN0000471818
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004987.1_s317c1
- Derived from:
- ccsbBroadEn_02716
- DNA Barcode:
- TCAATGTATACTGCAGATACCCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NMNAT2 (23057)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471818
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23057 | NMNAT2 | nicotinamide nucleotide ade... | NM_170706.3 | 100% | 100% | |
| 2 | human | 23057 | NMNAT2 | nicotinamide nucleotide ade... | NM_015039.4 | 93.2% | 90.3% | (many diffs) |
| 3 | human | 23057 | NMNAT2 | nicotinamide nucleotide ade... | XM_024454277.1 | 79.1% | 79.1% | 0_1ins189 |
| 4 | mouse | 226518 | Nmnat2 | nicotinamide nucleotide ade... | XM_006529455.3 | 85.7% | 90.7% | (many diffs) |
| 5 | mouse | 226518 | Nmnat2 | nicotinamide nucleotide ade... | NM_175460.3 | 85% | 89.1% | (many diffs) |
| 6 | mouse | 226518 | Nmnat2 | nicotinamide nucleotide ade... | XR_387163.3 | 17.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 972
- ORF length:
- 906
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aatccaggaa ctagaggaaa ttcaggcctg ccaggggctc tgggaagtgt 121 ttgttactct ctcagaaaga gccagggatt atctgcacaa aactggaagg tttattgtga 181 ttggcgggat tgtctcccct gtccacgact cctatggaaa acagggcctc gtgtcaagcc 241 ggcaccgtct catcatgtgt cagctggccg tccagaattc tgattggatc agggtggacc 301 cttgggagtg ctaccaggac acctggcaga cgacctgcag cgtgttggaa caccaccggg 361 acctcatgaa gagggtgact ggctgcatcc tctccaatgt caacacacct tccatgacac 421 ctgtgatcgg acagccacaa aacgagaccc cccagcccat ttaccagaac agcaacgtgg 481 ccaccaagcc cactgcagcc aagatcttgg ggaaggtggg agaaagccTC AGCCGGATCT 541 GCTGTGTCCG CCCGCCGGTG GAGCGTTTCA CCTTTGTAGA TGAGAATGCC AATCTGGGCA 601 CGGTGATGCG GTATGAAGAG ATTGAGCTAC GGATCCTGCT GCTGTGTGGT AGTGACCTGC 661 TGGAGTCCTT CTGCATCCCA GGGCTCTGGA ACGAGGCAGA TATGGAGGTG ATTGTTGGTG 721 ACTTTGGGAT TGTGGTGGTG CCCCGGGATG CAGCCGACAC AGACCGAATC ATGAATCACT 781 CCTCAATACT CCGCAAATAC AAAAACAACA TCATGGTGGT GAAGGATGAC ATCAACCATC 841 CCATGTCTGT TGTCAGCTCA ACCAAGAGCA GGCTGGCCCT GCAGCATGGG GACGGCCATG 901 TTGTGGATTA CCTGTCCCAG CCGGTCATCG ACTACATCCT CAAAAGCCAG CTGTACATCA 961 ATGCCTCCGG CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1021 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1081 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATCA ATGTATACTG CAGATACCCG 1141 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t