Transcript: Human XM_024454277.1

PREDICTED: Homo sapiens nicotinamide nucleotide adenylyltransferase 2 (NMNAT2), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NMNAT2 (23057)
Length:
5485
CDS:
362..1081

Additional Resources:

NCBI RefSeq record:
XM_024454277.1
NBCI Gene record:
NMNAT2 (23057)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024454277.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000035442 GAGGTGATTGTTGGTGACTTT pLKO.1 812 CDS 100% 4.950 6.930 N NMNAT2 n/a
2 TRCN0000035441 CACTCCTCAATACTCCGCAAA pLKO.1 884 CDS 100% 4.050 5.670 N NMNAT2 n/a
3 TRCN0000035440 GCCAGGGATTATCTGCACAAA pLKO.1 248 5UTR 100% 4.950 3.465 N NMNAT2 n/a
4 TRCN0000035439 CGGTATGAAGAGATTGAGCTA pLKO.1 716 CDS 100% 2.640 1.848 N NMNAT2 n/a
5 TRCN0000035443 GCCCATTTACCAGAACAGCAA pLKO.1 562 CDS 100% 2.640 1.848 N NMNAT2 n/a
6 TRCN0000111477 CCCATTTACCAGAACAGCAAT pLKO.1 563 CDS 100% 4.950 3.960 N Nmnat2 n/a
7 TRCN0000166364 CACACACACACACACACACAA pLKO.1 3991 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024454277.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02716 pDONR223 100% 79.1% 79.1% None 0_1ins189 n/a
2 ccsbBroad304_02716 pLX_304 0% 79.1% 79.1% V5 0_1ins189 n/a
3 TRCN0000471818 TCAATGTATACTGCAGATACCCGA pLX_317 49% 79.1% 79.1% V5 0_1ins189 n/a
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