Construct: ORF TRCN0000471830
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014669.1_s317c1
- Derived from:
- ccsbBroadEn_06350
- DNA Barcode:
- AGGAGCATCGCCCTTGGGTCGAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HAL (3034)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471830
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3034 | HAL | histidine ammonia-lyase | NM_002108.4 | 99.9% | 99.8% | 1315G>A |
| 2 | human | 3034 | HAL | histidine ammonia-lyase | NM_001258334.2 | 89.9% | 89.4% | 1315G>A;1760_1761ins70;1773_1774ins128 |
| 3 | human | 3034 | HAL | histidine ammonia-lyase | NM_001258333.1 | 68.2% | 68.1% | 0_1ins624;691G>A |
| 4 | human | 3034 | HAL | histidine ammonia-lyase | XM_011538249.2 | 56.7% | 56.4% | 0_1ins844;3_4insCTAAATAC;463G>A |
| 5 | human | 3034 | HAL | histidine ammonia-lyase | XM_017019246.1 | 52.7% | 52.6% | 0_1ins930;385G>A |
| 6 | mouse | 15109 | Hal | histidine ammonia lyase | NM_010401.4 | 87.4% | 93.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2040
- ORF length:
- 1971
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcccagatac acggtgcacg tacgtgggga atggctggca gtgccctgcc 121 aggacgcgca gctcactgtg ggctggctgg gccgggaggc cgtgaggcgc tatatcaaga 181 ataagcccga caatggtggc ttcacctccg tggatgacgc gcacttcctt gtgcgccggt 241 gcaagggcct gggcctgctg gacaacgagg accggctcga ggtggcccta gagaacaacg 301 agttcgtgga agtggttata gagggtgatg ccatgtctcc tgacttcatt ccatctcaac 361 cagaaggagt ttatctatac agcaagtacc gggagcctga aaagtacatc gagttagatg 421 gagaccgtct gaccacggag gatctggtca acttgggaaa gggacgctac aaaataaagc 481 tcaccccaac agctgagaag agggtgcaga aatccaggga ggtcatagat agcatcataa 541 aagagaaaac agttgtttac ggtattacta caggttttgg gaaatttgcc agaactgtaa 601 ttcctatcaa taagctacag gagcttcagg tcaacttagt acgctcacat tcttcaggtg 661 ttgggaaacc actaagtcct gagaggtgtc ggatgctctt ggctttaagg atcaatgtct 721 tagccaaagg atacagtggc atttccctgg agaccctcaa acaagtcata gaaatgttta 781 atgcctcctg cctgccctat gtcccagaga aaggaaccgt tggtgccagt ggagaccttg 841 ccccactctc tcatcttgct cttgggctag ttggagaagg gaagatgtgg tctccgaaga 901 gtggctgggc tgatgctaaa tacgtgctag aagcccatgg attgaaacca gttattttaa 961 aaccaaaaga gggcctggca ctcatcaatg ggacgcagat gatcacatcc ctgggctgtg 1021 aagctgtaga gcgagccagt gctattgcac ggcaggctga cattgtggca gccctgaccc 1081 ttgaggtgct gaagggcacc accaaagcct ttgacactga cattcatgct cttcgacctc 1141 accgtgggca aattgaagtt gcttttcggt ttcggtcact cttggactca gatcaccacc 1201 catcagaaat agcagagagt cacaggttct gtgatcgcgt ccaggatgca tacaccttgc 1261 gctgctgtcc acaggtccat ggtgtggtga atgatacaat agcatttgtg aagaacatca 1321 ttaccacaga actgaacagc gcaacagata atcctatggt ctttgccaat aggggagaga 1381 caatttctgg aggaaacttc catggtgaat acccagccaa agccctagac tacttggcca 1441 ttggcatcca tgaacttgct gcaatcagtg agagaagaat cgagcggctc tgcaatccct 1501 ccctcagtga gctgcctgcc ttcctggtgg ctgaaggtgg tctgaactct GGGTTCATGA 1561 TAGCTCACTG CACGGCAGCA GCCCTTGTTT CTGAGAACAA GGCTCTGTGC CATCCCTCGT 1621 CTGTTGACTC CCTCTCCACC AGCGCAGCCA CGGAGGACCA CGTCTCCATG GGAGGATGGG 1681 CAGCAAGGAA AGCCCTCAGG GTCATCGAGC ATGTGGAGCA AGTGCTGGCC ATCGAGCTCC 1741 TTGCAGCCTG CCAGGGCATA GAGTTTCTAC GTCCCCTGAA AACAACCACT CCGCTGGAGA 1801 AGGTCTATGA CCTGGTGCGC TCTGTTGTAA GGCCCTGGAT AAAAGATCGC TTCATGGCCC 1861 CGGACATCGA GGCAGCCCAC AGGCTGCTCC TGGAGCAGAA GGTTTGGGAA GTAGCTGCTC 1921 CATACATTGA AAAATACAGA ATGGAGCATA TTCCAGAATC AAGACCTCTT TCTCCAACAG 1981 CCTTTTCACT GCAATTTCTG CACAAGAAAT CCACCAAAAT CCCGGAGTCT GAGGACCTTT 2041 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2101 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2161 TTTATATATC TTGTGGAAAG GACGAAGGAG CATCGCCCTT GGGTCGAAAA CGCGTTAAGT 2221 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt