Construct: ORF TRCN0000471845

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010872.1_s317c1
Derived from:
ccsbBroadEn_08836
DNA Barcode:
ATCCTTATGAAAAAATCTTTTGTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TMBIM1 (64114)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471845
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64114 TMBIM1 transmembrane BAX inhibitor... NM_001321427.1 99.8% 99.6% 62C>T
2 human 64114 TMBIM1 transmembrane BAX inhibitor... NM_001321428.1 99.8% 99.6% 62C>T
3 human 64114 TMBIM1 transmembrane BAX inhibitor... NM_001321429.1 99.8% 99.6% 62C>T
4 human 64114 TMBIM1 transmembrane BAX inhibitor... NM_001321430.1 99.8% 99.6% 62C>T
5 human 64114 TMBIM1 transmembrane BAX inhibitor... NM_001321432.1 99.8% 99.6% 62C>T
6 human 64114 TMBIM1 transmembrane BAX inhibitor... NM_001321433.1 99.8% 99.6% 62C>T
7 human 64114 TMBIM1 transmembrane BAX inhibitor... NM_001321435.1 99.8% 99.6% 62C>T
8 human 64114 TMBIM1 transmembrane BAX inhibitor... NM_001321436.1 99.8% 99.6% 62C>T
9 human 64114 TMBIM1 transmembrane BAX inhibitor... NM_022152.6 99.7% 99.6% 62C>T;237A>G
10 human 64114 TMBIM1 transmembrane BAX inhibitor... XM_011511627.1 99.7% 99.6% 62C>T;237A>G
11 human 64114 TMBIM1 transmembrane BAX inhibitor... NM_001321438.1 64.3% 60.7% 0_1ins268;35_36ins65
12 human 64114 TMBIM1 transmembrane BAX inhibitor... NR_135643.1 35.9% (many diffs)
13 mouse 69660 Tmbim1 transmembrane BAX inhibitor... NM_027154.5 84.6% 88.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
999
ORF length:
933
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc caaccccagc gccccaccac catatgaaga ccgcaacccc ctgtacccag
121gccctctgcc ccctgggggc tatgggcagc catctgtcct gccaggaggg tatcctgcct
181accctggcta cccgcagcct ggctacggtc accctgctgg ctacccacag cccatgcccc
241ccacccaccc gatgcccatg aactacggcc caggccatgg ctatgatggg gaggagagag
301cggtgagtga tagcttcggg cctggagagt gggatgaccg gaaagtgcga cacactttta
361tccgaaaggt ttactccatc atctccgtgc agctgctcat cactgtggcc atcattgcta
421tcttcacctt tgtggaacct gtcagcgcct ttgtgaggag aaatgtggct gtctactacg
481tgtcctatgc tgtcttcgtt gtcacctacc tgatccttgc ctgctgccag ggacccagac
541gccgtttccc atggaacatc attctgctga ccctttttac ttttgccatg ggcttcatga
601cgggcaccat TTCCAGTATG TACCAAACCA AAGCCGTCAT CATTGCAATG ATCATCACTG
661CGGTGGTATC CATTTCAGTC ACCATCTTCT GCTTTCAGAC CAAGGTGGAC TTCACCTCGT
721GCACAGGCCT CTTCTGTGTC CTGGGAATTG TGCTCCTGGT GACTGGGATT GTCACTAGCA
781TTGTGCTCTA CTTCCAATAC GTTTACTGGC TCCACATGCT CTATGCTGCT CTGGGGGCCA
841TTTGTTTCAC CCTGTTCCTG GCTTACGACA CACAGCTGGT CCTGGGGAAC CGGAAGCACA
901CCATCAGCCC CGAGGACTAC ATCACTGGCG CCCTGCAGAT TTACACAGAC ATCATCTACA
961TCTTCACCTT TGTGCTGCAG CTGATGGGGG ATCGCAATTG CCCAACTTTC TTGTACAAAG
1021TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
1081GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
1141ACGAATCCTT ATGAAAAAAT CTTTTGTTAC GCGTTAAGTC gacaatcaac ctctggatta
1201caaaatttgt gaaagatt

Download FASTA (ORF) (Full)