Construct: ORF TRCN0000471848
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018333.1_s317c1
- Derived from:
- ccsbBroadEn_12505
- DNA Barcode:
- TTTCGGCTTCCCCGAACACATCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPS11 (64963)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471848
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64963 | MRPS11 | mitochondrial ribosomal pro... | NM_001321970.2 | 100% | 100% | |
| 2 | human | 64963 | MRPS11 | mitochondrial ribosomal pro... | NM_022839.5 | 99.4% | 99.4% | 65_67delGCA |
| 3 | human | 64963 | MRPS11 | mitochondrial ribosomal pro... | NM_176805.4 | 82.4% | 82.4% | 65_67delGCA;182_183ins99 |
| 4 | human | 64963 | MRPS11 | mitochondrial ribosomal pro... | NM_001321972.2 | 77.9% | 72.5% | (many diffs) |
| 5 | human | 64963 | MRPS11 | mitochondrial ribosomal pro... | NM_001321974.2 | 34.1% | 31.4% | (many diffs) |
| 6 | human | 64963 | MRPS11 | mitochondrial ribosomal pro... | NR_135917.2 | 16.4% | (many diffs) | |
| 7 | human | 64963 | MRPS11 | mitochondrial ribosomal pro... | NR_135915.2 | 15.3% | (many diffs) | |
| 8 | human | 64963 | MRPS11 | mitochondrial ribosomal pro... | NR_135918.2 | 15.2% | (many diffs) | |
| 9 | mouse | 67994 | Mrps11 | mitochondrial ribosomal pro... | XM_006541135.3 | 51.9% | 50.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 645
- ORF length:
- 579
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ggctgtgaga aacgcggggt cgcggttcct gcggtcctgg acttggcccc 121 agacagccgg ggTCGTGGCC AGAACGCCGG CCGGGACCAT CTGCACAGGC GCTCGACAGC 181 TCCAAGACGC TGCGGCCAAG CAGAAAGTTG AACAGAACGC GGCTCCCAGC CACACCAAGT 241 TCAGCATTTA CCCTCCCATT CCAGGAGAGG AGAGCTCTCT GAGGTGGGCA GGAAAGAAAT 301 TTGAGGAGAT CCCAATTGCA CACATTAAAG CATCCCACAA CAACACACAG ATCCAGGTAG 361 TCTCTGCTAG TAATGAGCCC CTTGCCTTTG CTTCCTGTGG CACAGAGGGA TTTCGGAATG 421 CCAAGAAGGG CACAGGCATC GCAGCACAGA CAGCAGGCAT AGCCGCAGCG GCGAGAGCTA 481 AACAAAAGGG CGTGATCCAC ATCCGAGTTG TGGTGAAAGG CCTGGGGCCA GGACGCTTGT 541 CTGCCATGCA CGGACTGATC ATGGGCGGCC TGGAAGTGAT CTCAATCACA GACAACACCC 601 CAATCCCACA CAACGGCTGC CGCCCCAGGA AGGCTCGGAA GCTGTGCCCA ACTTTCTTGT 661 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 721 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 781 GAAAGGACGA TTTCGGCTTC CCCGAACACA TCATACGCGT TAAGTCgaca atcaacctct 841 ggattacaaa atttgtgaaa gatt