Construct: ORF TRCN0000471866
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006613.1_s317c1
- Derived from:
- ccsbBroadEn_08583
- DNA Barcode:
- TCCGCGTTGTTACTGGTCATCAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IFT122 (55764)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471866
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55764 | IFT122 | intraflagellar transport 122 | NM_052990.3 | 99.9% | 100% | 1650A>T |
| 2 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_017006829.2 | 99.8% | 99.9% | 1650A>T;2456_2457insAGC |
| 3 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_017006833.2 | 98.3% | 98.4% | 1650A>T;1657_1658ins54 |
| 4 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_017006834.2 | 98.2% | 98.3% | 1650A>T;1657_1658ins54;2402_2403insAGC |
| 5 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_017006828.2 | 96.4% | 95.6% | (many diffs) |
| 6 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_005247609.2 | 95.5% | 95.6% | 192_347del;1806A>T |
| 7 | human | 55764 | IFT122 | intraflagellar transport 122 | NM_018262.4 | 95.4% | 95.5% | 192_347del;1806A>T;2612_2613insAGC |
| 8 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_006713692.3 | 94% | 94% | 192_347del;1806A>T;1813_1814ins54 |
| 9 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_017006827.2 | 93.9% | 93.9% | (many diffs) |
| 10 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_017006823.2 | 92.3% | 91.5% | (many diffs) |
| 11 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_017006824.2 | 92.2% | 91.4% | (many diffs) |
| 12 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_017006822.2 | 91.8% | 90.9% | (many diffs) |
| 13 | human | 55764 | IFT122 | intraflagellar transport 122 | NM_001280541.2 | 91.6% | 91.6% | 192_500del;1959A>T |
| 14 | human | 55764 | IFT122 | intraflagellar transport 122 | NM_001280546.1 | 91.2% | 91.2% | 0_1ins294;1356A>T;2162_2163insAGC |
| 15 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_006713689.2 | 91% | 90.9% | 192_347del;564_740del;1983A>T |
| 16 | human | 55764 | IFT122 | intraflagellar transport 122 | NM_052989.3 | 90.9% | 90.9% | (many diffs) |
| 17 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_017006825.2 | 90.8% | 90% | (many diffs) |
| 18 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_017006826.2 | 90.7% | 89.9% | (many diffs) |
| 19 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_017006836.2 | 89.7% | 89.7% | 0_1ins294;1356A>T;1363_1364ins54 |
| 20 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_006713691.3 | 89.5% | 89.5% | (many diffs) |
| 21 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_017006821.2 | 89.5% | 89.4% | (many diffs) |
| 22 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_017006817.2 | 88% | 87.1% | (many diffs) |
| 23 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_017006835.1 | 88% | 87.1% | (many diffs) |
| 24 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_017006818.2 | 88% | 87.1% | (many diffs) |
| 25 | human | 55764 | IFT122 | intraflagellar transport 122 | NM_052985.4 | 87.3% | 87.3% | (many diffs) |
| 26 | human | 55764 | IFT122 | intraflagellar transport 122 | NM_001280545.1 | 86.6% | 86.6% | (many diffs) |
| 27 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_017006819.2 | 86.6% | 85.7% | (many diffs) |
| 28 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_017006820.2 | 86.5% | 85.7% | (many diffs) |
| 29 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_017006830.2 | 83.8% | 82.8% | (many diffs) |
| 30 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_017006831.1 | 83.8% | 82.8% | (many diffs) |
| 31 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_017006832.1 | 83.8% | 82.8% | (many diffs) |
| 32 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_024453639.1 | 81.2% | 80.1% | (many diffs) |
| 33 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_017006838.2 | 68.3% | 68.3% | 0_1ins1071;579A>T;1385_1386insAGC |
| 34 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_011512972.3 | 68.1% | 65.9% | (many diffs) |
| 35 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_017006837.1 | 65.7% | 64.8% | (many diffs) |
| 36 | human | 55764 | IFT122 | intraflagellar transport 122 | XM_006713695.3 | 60.8% | 59.2% | (many diffs) |
| 37 | mouse | 81896 | Ift122 | intraflagellar transport 122 | NM_031177.4 | 84.1% | 87.4% | (many diffs) |
| 38 | mouse | 81896 | Ift122 | intraflagellar transport 122 | NM_001167763.1 | 84% | 87.3% | (many diffs) |
| 39 | mouse | 81896 | Ift122 | intraflagellar transport 122 | XM_006506797.2 | 80.1% | 83.1% | (many diffs) |
| 40 | mouse | 81896 | Ift122 | intraflagellar transport 122 | XM_006506798.2 | 80% | 83% | (many diffs) |
| 41 | mouse | 81896 | Ift122 | intraflagellar transport 122 | XM_006506799.3 | 76.1% | 78.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3459
- ORF length:
- 3393
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ggccgtgttg acgtggagag ataaagccga gcactgtata aatgacatcg 121 catttaagcc tgatggaact caactgattt tggctgccgg aagcagatta ctggtttatg 181 acacctctga tggcacctta cttcagcccc tcaagggaca caaagacact gtgtactgtg 241 tggcatatgc gaaggatggg ttgtggtctc ctgaacagaa gtctgtctcc aaacacaaat 301 caagcagcaa gatcatctgc tgcagctgga caaatgatgg tcagtacctg gcgctgggga 361 tgttcaatgg gatcatcagc atacggaaca aaaatggcga ggagaaagta aagatcgagc 421 ggccgggggg ctccctctcg ccaatatggt ccatctgctg gaacccttca agagaggaac 481 gtaatgacat cctggctgtg gctgactggg gacagaaagt ttccttctac cagctgagtg 541 gaaaacagat tggaaaggat cgggcactga actttgaccc ctgctgcatc agctacttta 601 ctaaaggcga gtacattttg ctggggggtt cagacaagca agtatctctt ttcaccaagg 661 atggagtgcg gcttgggact gttggggagc agaactcctg ggtgtggacg tgtcaagcga 721 aaccggattc caactatgtg gtggtcggct gccaggacgg caccatttcc ttctaccagc 781 ttattttcag cacagtccat gggctttaca aggaccgcta tgcctacagg gatagcatga 841 ctgacgtcat tgtgcagcac ctgatcactg agcagaaagt tcggattaaa tgcaaagagc 901 ttgtcaagaa gattgccatc tacagaaatc gattggctat ccaactgcca gagaaaatcc 961 tcatctatga gttgtattca gaggacttat cagacatgca ttaccgggta aaggagaaga 1021 ttatcaagaa gtttgagtgc aacctcctgg tggtgtgtgc caatcacatc atcctgtgcc 1081 aggagaaacg gctgcagtgc ctgtccttca gcggagtgaa ggagcgggag tggcagatgg 1141 agtctctcat tcgttacatc aaggtgatcg gtggccctcc tggaagagaa ggcctcttag 1201 tggggctgaa gaatggacag atcctgaaga tcttcgtgga caatctcttt gctatcgtcc 1261 tgctgaagca ggccacagct gtgcgctgct tggacatgag tgcctcccgt aagaagctgg 1321 ccgtggtaga tgaaaatgac acttgcctgg tgtatgacat cgacaccaag gagctgcttt 1381 ttcaggaacc aaacgccaac agtgtagctt ggaacaccca gtgtgaggac atgctctgct 1441 tctcgggagg aggctacctc aacatcaaag ccagcacctt ccctgtgcac cggcagaagc 1501 tgcagggctt tgtggtcggc tacaatggct ccaagatctt ctgcctccat gtcttctcca 1561 tttctgccgt ggaggtgccg cagtccgctc ccatgtacca gtacctggat aggaaactgt 1621 tcaaggaagc ctaccagatt gcttgcttgg gtgtcacaga cactgattgg cgtgaactgg 1681 ccatggaagc gctagaaggt ttagattttg aaactgcaaa gaaggccttc atcagagtac 1741 aagacctccg atatttagag ctcatcagca gcattgagga gaggaagaag cggggagaga 1801 ccaacaatga cctgtttctg gcagatgtgt tttcctacca ggggaagttc catgaggccg 1861 ccaaactgta caagaggagt gggcacgaga acctcgcgct tgaaatgtac accgacctct 1921 gcatgtttga gtatgccaag gatttccttg gatctggaga ccccaaagaa acaaagatgc 1981 taatcaccaa acaggctgac tgggccagaa atatcaagga gcccaaagcc gccgtggaga 2041 tgtacatctc agcaggagag cacgtcaagg ccatcgagat ctgtggtgac catggctggg 2101 ttgacatgtt gatcgacatc gcccgcaaac tggacaaggc tgagcgcgag cccctgctgc 2161 tgtgcgctac ctacctcaag aagctggaca gccctggcta tgctgctgag acctacctga 2221 agatgggtga cctcaagtcc ctggtgcagc tgcacgtgga gacccagcgc tgggatgagg 2281 cctttgcttt gggtgagaag catcctgagt ttaaggatga catctacatg ccgtatgctc 2341 agtggctagc agagaacgat cgctttgagg aagcccagaa agcgttccac aaggctgggc 2401 gacagagaga agcggtccag gtgctggagc agctcacaaa caatgccgtg gcggagagca 2461 ggtttaatga tgctgcctat tattactgga tgctgtccat gcagtgcctc gatatagctc 2521 aagcagatcc tgcccagaag gacacaatgc ttggcaagtt ctaccacttc cagcgtttgg 2581 cagagctgta ccatggttac catgccatcc atcgccacac ggaagatccg ttcagtgtcc 2641 atcgtcctga aactcttttc aacatctcca ggttcctgct gcacagcctg cccaaggaca 2701 ccccctcggg catctctaaa gtgaaaatac tcttcacctt ggccaagcag agcaaggccc 2761 tcggtgccta caggctggcc cggcacgcct atgacaagct gcgtggcctg tacatccctg 2821 ccagattcca aaagtccatt gagctgggta ccctgaccat ccgcgccaag cccttccacg 2881 acagtgagga gttggtgccc ttgtgctacc gctgctccac caacaacccg ctgctcaaca 2941 acctgggcaa cgtctgcatc aactgccgcc agcccttcat cttctccgcc tcttcctacg 3001 acgtgctaca cctggttgag ttctacctgg aggaagggat cactgatgaa gaagccatct 3061 ccctcatcga cctggaggtg ctgagaccca agcgggatga cagacagcta gagattgcaa 3121 acaacagctc ccagattctg cggctagtgg agaccaagga ctccatcgga gatgaggacc 3181 cgttcacagc taagctgagc tttgagcaag gtggctcaga gttcgtgcca gtggtggtga 3241 gccggctggt gcTGCGCTCC ATGAGCCGCC GGGATGTCCT CATCAAGCGA TGGCCCCCAC 3301 CCCTGAGGTG GCAATACTTC CGCTCACTGC TGCCTGACGC CTCCATTACC ATGTGCCCCT 3361 CCTGCTTCCA GATGTTCCAT TCTGAGGACT ATGAGTTGCT GGTGCTTCAG CATGGCTGCT 3421 GCCCCTACTG CCGCAGGTGC AAGGATGACC CTGGCCCATG CCCAACTTTC TTGTACAAAG 3481 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 3541 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 3601 ACGATCCGCG TTGTTACTGG TCATCAGAAC GCGTTAAGTC gacaatcaac ctctggatta 3661 caaaatttgt gaaagatt