Construct: ORF TRCN0000471869
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005817.1_s317c1
- Derived from:
- ccsbBroadEn_15582
- DNA Barcode:
- ACCTGATCCCCATTCCCTATATGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS17 (6218)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471869
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6218 | RPS17 | ribosomal protein S17 | NM_001021.6 | 99.7% | 100% | 312A>G |
| 2 | human | 6218 | RPS17 | ribosomal protein S17 | NR_111944.3 | 66.3% | (many diffs) | |
| 3 | human | 6218 | RPS17 | ribosomal protein S17 | NR_111943.2 | 51.5% | (many diffs) | |
| 4 | human | 79915 | ATAD5 | ATPase family AAA domain co... | XR_001752632.2 | 6.5% | (many diffs) | |
| 5 | human | 79915 | ATAD5 | ATPase family AAA domain co... | XR_001752631.2 | 6.1% | (many diffs) | |
| 6 | human | 79915 | ATAD5 | ATPase family AAA domain co... | XR_934553.3 | 5.9% | (many diffs) | |
| 7 | mouse | 20068 | Rps17 | ribosomal protein S17 | NM_009092.3 | 89.3% | 99.2% | (many diffs) |
| 8 | mouse | 623169 | Gm6402 | predicted pseudogene 6402; ... | NR_030688.1 | 26.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 471
- ORF length:
- 405
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ccgcgttcgc accaaaaCCG TGAAGAAGGC GGCCCGGGTC ATCATAGAAA 121 AGTACTACAC GCGCCTGGGC AACGACTTCC ACACGAACAA GCGCGTGTGC GAGGAGATCG 181 CCATTATCCC CAGCAAAAAG CTCCGCAACA AGATAGCAGG TTATGTCACG CATCTGATGA 241 AGCGAATTCA GAGAGGCCCA GTAAGAGGTA TCTCCATCAA GCTGCAGGAG GAGGAGAGAG 301 AAAGGAGAGA CAATTATGTT CCTGAGGTCT CAGCCTTGGA TCAGGAGATT ATTGAAGTAG 361 ATCCTGACAC TAAGGAGATG CTGAAGCTTT TGGACTTCGG CAGTCTGTCC AACCTTCAGG 421 TCACTCAGCC TACAGTTGGG ATGAATTTCA AAACGCCTCG GGGACCTGTT TGCCCAACTT 481 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 541 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 601 CTTGTGGAAA GGACGAACCT GATCCCCATT CCCTATATGT ACGCGTTAAG TCgacaatca 661 acctctggat tacaaaattt gtgaaagatt