Construct: ORF TRCN0000471879
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004624.2_s317c1
- Derived from:
- ccsbBroadEn_02350
- DNA Barcode:
- ATATGCAGCTGCTAATGATAACTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NME6 (10201)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471879
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | NM_005793.4 | 100% | 100% | |
| 2 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_017005513.2 | 97.9% | 96.9% | 4_5insCCCAGAATCTGG |
| 3 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | NM_001308426.1 | 95.8% | 95.8% | 0_1ins24 |
| 4 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | NM_001308427.1 | 95.8% | 95.8% | 0_1ins24 |
| 5 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | NM_001308428.1 | 95.8% | 95.8% | 0_1ins24 |
| 6 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_017005514.2 | 95.8% | 95.8% | 0_1ins24 |
| 7 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | NM_001308430.1 | 88.1% | 41.2% | 215_216ins40;513_514ins29 |
| 8 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | NM_001308431.1 | 84% | 37.7% | 0_1ins24;191_192ins40;489_490ins29 |
| 9 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | NM_001308433.1 | 84% | 37.7% | 0_1ins24;191_192ins40;489_490ins29 |
| 10 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_017005515.1 | 84% | 37.7% | 0_1ins24;191_192ins40;489_490ins29 |
| 11 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | NM_001308434.1 | 72.6% | 72.6% | 0_1ins159 |
| 12 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_017005516.2 | 66.1% | 39.2% | (many diffs) |
| 13 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_017005511.2 | 62% | 35.4% | (many diffs) |
| 14 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_024453300.1 | 61.9% | 50.5% | (many diffs) |
| 15 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | NM_001308435.1 | 61.3% | 61.3% | 0_1ins24;192_193ins201 |
| 16 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_024453301.1 | 60.5% | 48.4% | (many diffs) |
| 17 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_024453297.1 | 59.1% | 47.7% | (many diffs) |
| 18 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_024453302.1 | 59.1% | 47.7% | (many diffs) |
| 19 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_024453303.1 | 59.1% | 47.7% | (many diffs) |
| 20 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_024453304.1 | 59.1% | 47.7% | (many diffs) |
| 21 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_024453305.1 | 59.1% | 47.7% | (many diffs) |
| 22 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_024453306.1 | 59.1% | 47.7% | (many diffs) |
| 23 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_017005517.1 | 57.3% | 56.7% | (many diffs) |
| 24 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_017005512.2 | 57.2% | 42.6% | (many diffs) |
| 25 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_017005518.2 | 57.2% | 57.2% | 0_1ins249 |
| 26 | human | 10201 | NME6 | NME/NM23 nucleoside diphosp... | XM_024453299.1 | 54.8% | 45% | (many diffs) |
| 27 | mouse | 54369 | Nme6 | NME/NM23 nucleoside diphosp... | NM_018757.1 | 82.6% | 84.2% | (many diffs) |
| 28 | mouse | 54369 | Nme6 | NME/NM23 nucleoside diphosp... | XM_006512197.1 | 82.6% | 84.2% | (many diffs) |
| 29 | mouse | 54369 | Nme6 | NME/NM23 nucleoside diphosp... | XM_006512198.3 | 82.6% | 84.2% | (many diffs) |
| 30 | mouse | 54369 | Nme6 | NME/NM23 nucleoside diphosp... | XM_006512199.3 | 82.6% | 84.2% | (many diffs) |
| 31 | mouse | 54369 | Nme6 | NME/NM23 nucleoside diphosp... | XM_006512200.2 | 52.7% | 52.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 648
- ORF length:
- 582
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ccagaatctg gggagtgaga tggcctcaat cttgcgaagc cctcaggctc 121 tccagctcac tctagccctG ATCAAGCCTG ACGCAGTCGC CCATCCACTG ATTCTGGAGG 181 CTGTTCATCA GCAGATTCTA AGCAACAAGT TCCTGATTGT ACGAATGAGA GAACTACTGT 241 GGAGAAAGGA AGATTGCCAG AGGTTTTACC GAGAGCATGA AGGGCGTTTT TTCTATCAGA 301 GGCTGGTGGA GTTCATGGCC AGCGGGCCAA TCCGAGCCTA CATCCTTGCC CACAAGGATG 361 CCATCCAGCT CTGGAGGACG CTCATGGGAC CCACCAGAGT GTTCCGAGCA CGCCATGTGG 421 CCCCAGATTC TATCCGTGGG AGTTTCGGCC TCACTGACAC CCGCAACACC ACCCATGGTT 481 CGGACTCTGT GGTTTCAGCC AGCAGAGAGA TTGCAGCCTT CTTCCCTGAC TTCAGTGAAC 541 AGCGCTGGTA TGAGGAGGAA GAGCCCCAGT TGCGCTGTGG CCCTGTGTGC TATAGCCCAG 601 AGGGAGGTGT CCACTATGTA GCTGGAACAG GAGGCCTAGG ACCAGCCTGC CCAACTTTCT 661 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 721 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 781 GTGGAAAGGA CGAATATGCA GCTGCTAATG ATAACTAACG CGTTAAGTCg acaatcaacc 841 tctggattac aaaatttgtg aaagatt