Transcript: Mouse XM_006512198.3

PREDICTED: Mus musculus NME/NM23 nucleoside diphosphate kinase 6 (Nme6), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Nme6 (54369)
Length:
1180
CDS:
264..833

Additional Resources:

NCBI RefSeq record:
XM_006512198.3
NBCI Gene record:
Nme6 (54369)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006512198.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000221706 AGGCCACAAACAACCTAACAA pLKO.1 806 CDS 100% 5.625 3.938 N Nme6 n/a
2 TRCN0000345007 AGGCCACAAACAACCTAACAA pLKO_005 806 CDS 100% 5.625 3.938 N Nme6 n/a
3 TRCN0000221702 GCTGCAGTCCAGTTCTATCTA pLKO.1 973 3UTR 100% 5.625 3.938 N Nme6 n/a
4 TRCN0000345008 GCTGCAGTCCAGTTCTATCTA pLKO_005 973 3UTR 100% 5.625 3.938 N Nme6 n/a
5 TRCN0000221703 CAGATTCAATTCGTGGAAGTT pLKO.1 598 CDS 100% 4.950 3.465 N Nme6 n/a
6 TRCN0000345006 CAGATTCAATTCGTGGAAGTT pLKO_005 598 CDS 100% 4.950 3.465 N Nme6 n/a
7 TRCN0000221705 CCAACTTTGGAGGACACTGAT pLKO.1 539 CDS 100% 4.950 3.465 N Nme6 n/a
8 TRCN0000345079 CCAACTTTGGAGGACACTGAT pLKO_005 539 CDS 100% 4.950 3.465 N Nme6 n/a
9 TRCN0000221704 GCAGATTCTGAGCAACAAGTT pLKO.1 365 CDS 100% 4.950 3.465 N Nme6 n/a
10 TRCN0000010190 CCGAGAGCATGAAGGGCGTTT pLKO.1 443 CDS 100% 1.350 0.945 N NME6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006512198.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02350 pDONR223 100% 82.6% 84.2% None (many diffs) n/a
2 ccsbBroad304_02350 pLX_304 0% 82.6% 84.2% V5 (many diffs) n/a
3 TRCN0000471879 ATATGCAGCTGCTAATGATAACTA pLX_317 86.2% 82.6% 84.2% V5 (many diffs) n/a
4 ccsbBroadEn_14957 pDONR223 0% 82.6% 84.2% None (many diffs) n/a
5 ccsbBroad304_14957 pLX_304 0% 82.6% 84.2% V5 (many diffs) n/a
6 TRCN0000492136 CGACAGGAGTATCTTATTCTTTTT pLX_317 33.8% 82.6% 84.2% V5 (many diffs) n/a
7 TRCN0000491599 GGACTGGACCCGACACTCACGCAG pLX_317 45.8% 82.6% 84.2% V5 (not translated due to prior stop codon) (many diffs) n/a
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