Transcript: Human XM_024453299.1

PREDICTED: Homo sapiens NME/NM23 nucleoside diphosphate kinase 6 (NME6), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NME6 (10201)
Length:
1867
CDS:
90..998

Additional Resources:

NCBI RefSeq record:
XM_024453299.1
NBCI Gene record:
NME6 (10201)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146177 TACCTCCAGAATCAGTGGAT pXPR_003 GGG 208 23% 3 0.9385 NME6 NME6 76223
2 BRDN0001148253 TGTGGGCAAGGATGTAGGCT pXPR_003 CGG 380 42% 6 0.4024 NME6 NME6 76221
3 BRDN0001145496 AGTGTTCCGAGCACGCCATG pXPR_003 TGG 457 50% 6 0.1695 NME6 NME6 76222
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024453299.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000010191 CCAATCCGAGCCTACATCCTT pLKO.1 459 CDS 100% 3.000 4.200 N NME6 n/a
2 TRCN0000344507 CCAATCCGAGCCTACATCCTT pLKO_005 459 CDS 100% 3.000 4.200 N NME6 n/a
3 TRCN0000010190 CCGAGAGCATGAAGGGCGTTT pLKO.1 401 CDS 100% 1.350 1.890 N NME6 n/a
4 TRCN0000010188 CACTCTAGCCCTGATCAAGCC pLKO.1 260 CDS 100% 0.720 1.008 N NME6 n/a
5 TRCN0000344573 CACTCTAGCCCTGATCAAGCC pLKO_005 260 CDS 100% 0.720 1.008 N NME6 n/a
6 TRCN0000199549 CAGTCGCCCATCCACTGATTC pLKO.1 286 CDS 100% 3.600 2.520 N NME6 n/a
7 TRCN0000010189 GAACTACTGTGGAGAAAGGAA pLKO.1 363 CDS 100% 3.000 2.100 N NME6 n/a
8 TRCN0000199487 GCCTCAATCTTGCGAAGCCCT pLKO.1 225 CDS 100% 0.220 0.154 N NME6 n/a
9 TRCN0000166201 CATGGTGAAACCCTGTCTCTA pLKO.1 1003 3UTR 100% 4.950 2.475 Y ORAI2 n/a
10 TRCN0000136653 GCCTGACCAACATGGTGAAAT pLKO.1 993 CDS 100% 13.200 6.600 Y IQCC n/a
11 TRCN0000179120 CAACATGGTGAAACCCTGTTT pLKO.1 1000 3UTR 100% 4.950 2.475 Y LOC339059 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024453299.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02350 pDONR223 100% 54.8% 45% None (many diffs) n/a
2 ccsbBroad304_02350 pLX_304 0% 54.8% 45% V5 (many diffs) n/a
3 TRCN0000471879 ATATGCAGCTGCTAATGATAACTA pLX_317 86.2% 54.8% 45% V5 (many diffs) n/a
4 ccsbBroadEn_14957 pDONR223 0% 54.8% 45% None (many diffs) n/a
5 ccsbBroad304_14957 pLX_304 0% 54.8% 45% V5 (many diffs) n/a
6 TRCN0000492136 CGACAGGAGTATCTTATTCTTTTT pLX_317 33.8% 54.8% 45% V5 (many diffs) n/a
7 TRCN0000491599 GGACTGGACCCGACACTCACGCAG pLX_317 45.8% 54.8% 45% V5 (not translated due to prior stop codon) (many diffs) n/a
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