Construct: ORF TRCN0000471921
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018947.3_s317c1
- Derived from:
- ccsbBroadEn_14689
- DNA Barcode:
- TGAGAAAATAATCTTAGATAAACA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- LIMK1 (3984)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471921
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3984 | LIMK1 | LIM domain kinase 1 | NM_002314.4 | 99.5% | 32.5% | (many diffs) |
| 2 | human | 3984 | LIMK1 | LIM domain kinase 1 | NM_001204426.2 | 93.4% | 25.5% | (many diffs) |
| 3 | mouse | 16885 | Limk1 | LIM-domain containing, prot... | NM_010717.3 | 87.6% | 31.4% | (many diffs) |
| 4 | mouse | 16885 | Limk1 | LIM-domain containing, prot... | NM_001305875.1 | 82.2% | 23.5% | (many diffs) |
| 5 | mouse | 16885 | Limk1 | LIM-domain containing, prot... | XM_006504396.3 | 72.3% | 37.4% | (many diffs) |
| 6 | mouse | 16885 | Limk1 | LIM-domain containing, prot... | NR_131247.1 | 43.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 786
- ORF length:
- 717
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaggttgacg ctactttgtt gcacctggag ggaagaacgt atgggagagg 121 aaggaagcga gttgcccgtg tgtgcaagct gcggccagag gatctatgat ggccagtacc 181 tccaggccct gaacgcggac tggcacgcag actgcttcag gtgttgtgac tgcagtgcct 241 ccctgtcgca ccagtactat gagaaggatg ggcagctctt ctgcaagaag gactactggg 301 cccgctatgg cgagtcctgc catgggtgct ctgagcaaat caccaaggga ctggttatgg 361 tggctgggga gctgaagtac caccccgagt gtttcatctg cctcacgtgt gggaccttta 421 tcggtgacgg ggacacctac acgctggtgg agcactccaa gctgtactgc gggcactgct 481 actaccagac tgtggtgacc cccgtcatcg agcagatcct gcctgactcc cctggctccc 541 acctgcccca caccgtcacc ctggtgtcca tcccagcctc atctcatggc aagcgtggac 601 tttcagtctc cattgacccc ccgcacggcc caccgggctg tggcaccgag cactcacaca 661 ccgtccgcgt ccagggagtg gatcccgggc tgcatgagcc cagatgtgaa gaattccatc 721 cacgtcggag accggatctt ggaaatcaat ggcacgccca tccgaaatgt gcccctggac 781 gagattgacc tgctgattca ggaaaccagc cgcctgctcc agctgaccct cgagcatgac 841 cctcacgata cactgggcca cgggctgggg cctgagacca gccccctgag ctctccggct 901 tatactccca gcggggaggc gggcagctct gcccggcaga aacctgtctt gaggagctgc 961 agcatcgaca ggtctccggg cgctggctca ctgggctccc cggcctccca gcgcaaggac 1021 ctgggtcgct ctgagtccct ccgcgtagtc tgccggccac accgcatctt ccggccgtcg 1081 gacctcatcc acggggaggt gctgggcaag ggctgcttcg gccaggctat caaggtgaca 1141 caccgtgaga caggtgaggt gatggtgatg aaggagctga tccggttcga cgaggagacc 1201 cagaggacgt tcctcaagga ggtgaaggtc atgcgatgcc tggaacaccc caacgtgctc 1261 aagttcatcg gggtgctcta caggacagag ctcactcatc actgagtaca tcaaagggcg 1321 gcacgctccg gggcatcatc aagagcatgg acagccagta cccatggagc cagagagtga 1381 gctttgccaa ggacatcgca tcagggatgg cctacctcca ctccatgaac atcatccacc 1441 gagacctcaa ctcccacaac tgcctggtcc gcgagaacaa gaatgtggtg gtggctgact 1501 tcgggctggc gcgtctcatg gtggacgaga agactcagcc tgagggcctg cggagcctca 1561 agaagccaga ccgcaagaag cgctacaccg tggtgggcaa cccctactgg atggcacctg 1621 agatgatCAA CGGCCGCAGC TATGATGAGA AGGTGGATGT GTTCTCCTTT GGGATCGTCC 1681 TGTGCGAGAT CATCGGGCGG GTGAACGCAG ACCCTGACTA CCTGCCCCGC ACCATGGACT 1741 TTGGCCTCAA CGTGCGAGGA TTCCTGGACC GCTACTGCCC CCCAAACTGC CCCCCGAGCT 1801 TCTTCCCCAT CACCGTGCGC TGTTGCGATC TGGACCCCGA GAAGAGGCCA TCCTTTGTGA 1861 AGCTGGAACA CTGGCTGGAG ACCCTCCGCA TGCACCTGGC CGGCCACCTG CCACTGGGCC 1921 CACAGCTGGA GCAGCTGGAC AGAGGTTTCT GGGAGACCTA CCGGCGCGGC GAGAGCGGAC 1981 TGCCTGCCCA CCCTGAGGTC CCCGACTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 2041 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2101 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATGAGAAAA 2161 TAATCTTAGA TAAACAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2221 aagatt