Construct: ORF ccsbBroadEn_14689
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018947.1_s300c1, BRDN0000458243
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- LIMK1 (3984)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3984 | LIMK1 | LIM domain kinase 1 | NM_002314.4 | 99.5% | 32.5% | (many diffs) |
| 2 | human | 3984 | LIMK1 | LIM domain kinase 1 | NM_001204426.2 | 93.4% | 25.5% | (many diffs) |
| 3 | mouse | 16885 | Limk1 | LIM-domain containing, prot... | NM_010717.3 | 87.6% | 31.4% | (many diffs) |
| 4 | mouse | 16885 | Limk1 | LIM-domain containing, prot... | NM_001305875.1 | 82.2% | 23.5% | (many diffs) |
| 5 | mouse | 16885 | Limk1 | LIM-domain containing, prot... | XM_006504396.3 | 72.3% | 37.4% | (many diffs) |
| 6 | mouse | 16885 | Limk1 | LIM-domain containing, prot... | NR_131247.1 | 43.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 98
- ORF end:
- 815
- ORF length:
- 717
- Sequence:
-
1 AATGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCACCATG AGGTTGACGC TACTTTGTTG 121 CACCTGGAGG GAAGAACGTA TGGGAGAGGA AGGAAGCGAG TTGCCCGTGT GTGCAAGCTG 181 CGGCCAGAGG ATCTATGATG GCCAGTACCT CCAGGCCCTG AACGCGGACT GGCACGCAGA 241 CTGCTTCAGG TGTTGTGACT GCAGTGCCTC CCTGTCGCAC CAGTACTATG AGAAGGATGG 301 GCAGCTCTTC TGCAAGAAGG ACTACTGGGC CCGCTATGGC GAGTCCTGCC ATGGGTGCTC 361 TGAGCAAATC ACCAAGGGAC TGGTTATGGT GGCTGGGGAG CTGAAGTACC ACCCCGAGTG 421 TTTCATCTGC CTCACGTGTG GGACCTTTAT CGGTGACGGG GACACCTACA CGCTGGTGGA 481 GCACTCCAAG CTGTACTGCG GGCACTGCTA CTACCAGACT GTGGTGACCC CCGTCATCGA 541 GCAGATCCTG CCTGACTCCC CTGGCTCCCA CCTGCCCCAC ACCGTCACCC TGGTGTCCAT 601 CCCAGCCTCA TCTCATGGCA AGCGTGGACT TTCAGTCTCC ATTGACCCCC CGCACGGCCC 661 ACCGGGCTGT GGCACCGAGC ACTCACACAC CGTCCGCGTC CAGGGAGTGG ATCCCGGGCT 721 GCATGAGCCC AGATGTGAAG AATTCCATCC ACGTCGGAGA CCGGATCTTG GAAATCAATG 781 GCACGCCCAT CCGAAATGTG CCCCTGGACG AGATTGACCT GCTGATTCAG GAaaccagcc 841 gcctgctcca gctgaccctc gagcatgacc ctcacgatac actgggccac gggctggggc 901 ctgagaccag ccccctgagc tctccggctt atactcccag cggggaggcg ggcagctctg 961 cccggcagaa acctgtcttg aggagctgca gcatcgacag gtctccgggc gctggctcac 1021 tgggctcccc ggcctcccag cgcaaggacc tgggtcgctc tgagtccctc cgcgtagtct 1081 gccggccaca ccgcatcttc cggccgtcgg acctcatcca cggggaggtg ctgggcaagg 1141 gctgcttcgg ccaggctatc aaggtgacac accgtgagac aggtgaggtg atggtgatga 1201 aggagctgat ccggttcgac gaggagaccc agaggacgtt cctcaaggag gtgaaggtcA 1261 TGCGATGCCT GGAACACCCC AACGTGCTCA AGTTCATCGG GGTGCTCTAC AGGACAGAGC 1321 TCACTCATCA CTGAGTACAT CAAAGGGCGG CACGCTCCGG GGCATCATCA AGAGCATGGA 1381 CAGCCAGTAC CCATGGAGCC AGAGAGTGAG CTTTGCCAAG GACATCGCAT CAGGGATGGC 1441 CTACCTCCAC TCCATGAACA TCATCCACCG AGACCTCAAC TCCCACAACT GCCTGGTCCG 1501 CGAGAACAAG AATGTGGTGG TGGCTGACTT CGGGCTGGCG CGTCTCATGG TGGACGAGAA 1561 GACTCAGCCT GAGGGCCTGC GGAGCCTCAA GAAGCCAGAC CGCAAGAAGC GCTACACCGT 1621 GGTGGGCAAC CCCTACTGGA TGGCACCTGA GATGATCAAC GGCCGCAGCT ATGATGAGAA 1681 GGTGGATGTG TTCTCCTTTG GGATCGTCCT GTGCGAGATC ATCGGGCGGG TGAACGCAGA 1741 CCCTGACTAC CTGCCCCGCA CCATGGACTT TGGCCTCAAC GTGCGAGGAT TCCTGGACCG 1801 CTACTGCCCC CCAAACTGCC CCCCGAGCTT CTTCCCCATC ACCGTGCGCT GTTGCGATCT 1861 GGACCCCGAG AAGAGGCCAT CCTTTGTGAA GCTGGAACAC TGGCTGGAGA CCCTCCGCAT 1921 GCACCTGGCC GGCCACCTGC CACTGGGCCC ACAGCTGGAG CAGCTGGACA GAGGTTTCTG 1981 GGAGACCTAC CGGCGCGGCG AGAGCGGACT GCCTGCCCAC CCTGAGGTCC CCGACTTGCC 2041 AACTTTCTTG TACAAAGTTG GCATTATAAG AAAGCATTGC TTATCAATTT GTTGCAACGA 2101 ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTGCCATC CAGCTAACTC AAG