Construct: ORF TRCN0000471923
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018843.3_s317c1
- Derived from:
- ccsbBroadEn_15020
- DNA Barcode:
- GACCCGCTTCGCATAAACTTACGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLXNB2 (23654)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471923
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23654 | PLXNB2 | plexin B2 | NM_012401.4 | 25.4% | 23.8% | (many diffs) |
| 2 | human | 23654 | PLXNB2 | plexin B2 | XM_005261909.1 | 25.4% | 23.8% | (many diffs) |
| 3 | human | 23654 | PLXNB2 | plexin B2 | XM_005261910.3 | 25.4% | 23.8% | (many diffs) |
| 4 | human | 23654 | PLXNB2 | plexin B2 | XM_005261911.4 | 25.4% | 23.8% | (many diffs) |
| 5 | human | 23654 | PLXNB2 | plexin B2 | XM_006724413.2 | 25.4% | 23.8% | (many diffs) |
| 6 | human | 23654 | PLXNB2 | plexin B2 | XM_017028703.1 | 25.4% | 23.8% | (many diffs) |
| 7 | human | 23654 | PLXNB2 | plexin B2 | XM_017028704.2 | 25.4% | 23.8% | (many diffs) |
| 8 | human | 23654 | PLXNB2 | plexin B2 | XM_011530682.2 | 24.6% | 23% | (many diffs) |
| 9 | mouse | 140570 | Plxnb2 | plexin B2 | NM_001159521.2 | 22.7% | 23.3% | (many diffs) |
| 10 | mouse | 140570 | Plxnb2 | plexin B2 | NM_001284506.1 | 22.7% | 23.3% | (many diffs) |
| 11 | mouse | 140570 | Plxnb2 | plexin B2 | NM_138749.3 | 22.7% | 23.3% | (many diffs) |
| 12 | mouse | 140570 | Plxnb2 | plexin B2 | XM_006520412.1 | 22.7% | 23.3% | (many diffs) |
| 13 | mouse | 140570 | Plxnb2 | plexin B2 | XM_006520413.1 | 22.7% | 23.3% | (many diffs) |
| 14 | mouse | 140570 | Plxnb2 | plexin B2 | XM_011245449.1 | 22.7% | 23.3% | (many diffs) |
| 15 | mouse | 140570 | Plxnb2 | plexin B2 | XM_011245450.2 | 22.7% | 23.3% | (many diffs) |
| 16 | mouse | 140570 | Plxnb2 | plexin B2 | XM_011245451.2 | 22.7% | 23.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1479
- ORF length:
- 1410
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcacacgctc ttcctggagc tcctggagca gtacgtggtg gccaagaacc 121 ccaagctgat gctgcgcagg tctgagactg tggtggagag gatgctgtcc aactggatgt 181 ccatctgcct gtaccagtac ctcaaggaca gtgccgggga gcccctgtac aagctcttca 241 aggccatcaa acatcaggtg gaaaagggcc cggtggatgc ggtacagaag aaggccaagt 301 acactctcaa cgacacgggg ctgctggggg atgatgtgga gtacgcaccc ctgacggtga 361 gcgtgatcgt gcaggacgag ggagtggacg ccatcccggt gaaggtcctc aactgtgaca 421 ccatctccca ggtcaaggag aagatcattg accaggtgta ccgtgggcag ccctgctcct 481 gctggcccag gccagacagc gtggtcctgg agtggcgtcc gggctccaca gcgcagatcc 541 tgtcggacct ggacctgacg tcacagcggg agggccggtg gaagcgcgtc aacaccctta 601 tgcactacaa tgtccgggat ggagccaccc tcatcctgtc caaggtgggg gtctcccagc 661 agccggagga cagccagcag gacctgcctg gggagcgcca tgccctcctg gagnagnaga 721 acccgggtgt ggcacctggt gcggccgacc gacgaggtgg acgagggcaa gtccaagaga 781 ggcagcgtga aagagaagga gcggacgaag gccatcaccg agatctactg acgcggctgc 841 tctcagtcaa gggcacactg cagcagtttg tggacaactt cttccagagc gtgctggcgc 901 ctgggcacgc ggtgccacct gcagtcaagt acttcttcga cttcctggac gagcaggcag 961 agaagcacaa catccaggat gaagacacca tccacatctg gaagacgaac agtttaccgc 1021 tccggttctg ggtgaacatc ctcaagaacc cccacttcat ctttgacgtg catgtccacg 1081 aggtggtgga cgcctcgctg tcagtcatcg cgcagacctt catggatgcc tgcacgcgca 1141 cggagcataa gcTGAGCCGC GATTCTCCCA GCAACAAGCT GCTGTACGCC AAGGAGATCT 1201 CCACCTACAA GAAGATGGTG GAGGATTACT ACAAGGGGAT CCGGCAGATG GTGCAGGTCA 1261 GCGACCAGGA CATGAACACA CACCTGGCAG AGATTTCCCG GGCGCACACG GACTCCTTGA 1321 ACACCCTCGT GGCACTCCAC CAGCTCTACC AATACACGCA GAAGTACTAT GACGAGATCA 1381 TCAATGCCTT GGAGGAGGAT CCTGCCGCCC AGAAGATGCA GCTGGCCTTC CGCCTGCAGC 1441 AGATTGCCGC TGCACTGGAG AACAAGGTCA CTGACCTCTT GCCAACTTTC TTGTACAAAG 1501 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1561 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1621 ACGAGACCCG CTTCGCATAA ACTTACGTAC GCGTTAAGTC gacaatcaac ctctggatta 1681 caaaatttgt gaaagatt