Construct: ORF TRCN0000471944
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001414.1_s317c1
- Derived from:
- ccsbBroadEn_08392
- DNA Barcode:
- ACTAAGCTACCCAAAATGATGTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EXOC6 (54536)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471944
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54536 | EXOC6 | exocyst complex component 6 | NM_019053.6 | 99.9% | 99.7% | 1187C>T;2326A>G |
| 2 | human | 54536 | EXOC6 | exocyst complex component 6 | NM_001319195.2 | 99.7% | 99.6% | 1187C>T;1414_1415insAGC;2323A>G |
| 3 | human | 54536 | EXOC6 | exocyst complex component 6 | NM_001013848.3 | 97% | 97% | (many diffs) |
| 4 | human | 54536 | EXOC6 | exocyst complex component 6 | XM_017016345.2 | 97% | 97% | (many diffs) |
| 5 | human | 54536 | EXOC6 | exocyst complex component 6 | XM_017016346.2 | 96.9% | 96.8% | (many diffs) |
| 6 | human | 54536 | EXOC6 | exocyst complex component 6 | XM_017016347.2 | 96.2% | 95.7% | (many diffs) |
| 7 | human | 54536 | EXOC6 | exocyst complex component 6 | NM_001319194.2 | 95.9% | 94.5% | (many diffs) |
| 8 | human | 54536 | EXOC6 | exocyst complex component 6 | XM_017016344.2 | 95.8% | 94.4% | (many diffs) |
| 9 | human | 54536 | EXOC6 | exocyst complex component 6 | XM_024448047.1 | 94% | 93.9% | 0_1ins141;1046C>T;2185A>G |
| 10 | human | 54536 | EXOC6 | exocyst complex component 6 | NM_001319200.1 | 87.1% | 87% | 818_819ins69;902_903ins240;2017A>G |
| 11 | human | 54536 | EXOC6 | exocyst complex component 6 | XM_017016348.1 | 87% | 84.1% | (many diffs) |
| 12 | mouse | 107371 | Exoc6 | exocyst complex component 6 | NM_175353.2 | 88.7% | 93.2% | (many diffs) |
| 13 | mouse | 107371 | Exoc6 | exocyst complex component 6 | XM_017318044.1 | 86% | 89.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2478
- ORF length:
- 2412
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggagaacagc gagagtctgg gcaccgtccc cgagcacgag cggatcttgc 121 aggagatcga gagcaccgac accgcctgtg tggggcccac cctccggtct gtgtatgatg 181 accaaccaaa tgcgcacaag aagtttatgg aaaagttaga tgcttgtatc cgtaatcatg 241 acaaggaaat tgaaaagatg tgtaattttc atcatcaggg ttttgtagat gctattacag 301 aactccttaa agtaaggact gatgcagaaa aactgaaggt gcaagttact gataccaacc 361 gaaggtttca agatgctgga aaagaggtga tagtccacac agaagatatc attcgatgta 421 gaattcagca gagaaatatt acaactgtag tagaaaaatt gcagttatgc cttcctgtgc 481 tagaaatgta cagtaagctg aaagaacaga tgagtgccaa aaggtactat tctgccctaa 541 aaactatgga acaattagag aatgtgtact ttccctgggt tagtcaatac cggttttgtc 601 agctcatgat agaaaatctt cccaaactcc gtgaggatat taaagaaatc tccatgtctg 661 atctcaaaga ctttttggaa agtattcgaa aacattctga caaaataggt gaaacagcaa 721 tgaaacaggc acagcatcag aaaaccttca gtgtttctct gcagaaacaa aataaaatga 781 aatttgggaa aaatatgtat ataaatcgtg atagaattcc agaggaaagg aatgaaactg 841 tattgaaaca ttcacttgaa gaagaggatg agaatgaaga agagatctta actgttcagg 901 atcttgttga tttttcccct gtttatcgat gtttgcacat ttattctgtt ttgggtgacg 961 aggaaacatt tgaaaactat tatcgaaaac aaagaaagaa acaagcaaga ctggtattgc 1021 aaccccagtc gaatatgcat gaaacagttg atggctatag aagatatttc actcaaattg 1081 tagggttctt tgtggtagaa gatcacattt tacatgtgac ccaaggatta gtaaccaggg 1141 catacactga tgaactttgg aacatggccc tctcaaagat aattgctgtc cttagagctc 1201 attcatccta ttgcactgat cctgatcttg ttctggagct gaagaatctt attgtaatat 1261 ttgcagatac tttacagggt tatggttttc cagtgaaccg actttttgac cttttatttg 1321 aaataagaga ccaatacaat gaaacactgc ttaagaaatg ggctggagtt ttcagggaca 1381 tttttgaaga agataattac agccccatcc ctgttgtcaa tgaagaagaa tataaaattg 1441 tcatcagcaa atttcccttt caagatccag accttgaaaa gcagtctttc ccaaagaaat 1501 tccccatgtc tcagtcagtg cctcatattt acattcaagt taaagaattt atttatgcca 1561 gccttaaatt ttcagagtca ctacaccgga gctcaacaga aatagacgat atgcttagaa 1621 aatcaacaaa tctgctgctg accagaactt tgagtagctg tttactgaac cttattagaa 1681 aacctcatat aggtttgaca gagctggtac aaatcatcat aaacacaaca cacctggagc 1741 aagcttgtaa atatcttgag gactttataa ctaacattac aaatatttcc caagaaactg 1801 ttcatactac aagactttat ggactttcta ctttcaagga tgctcgacat gcagcagaag 1861 gagaaatata taccaaactg aatcaaaaaa ttgatgaatt tgttcagctt gctgattatg 1921 actggacaat gtctgagcca gatggaagag ctagtggtta tttaatggac cttataaatt 1981 ttttgagaag catctttcaa gtgtttactc atttgcctgg gaaagttgct cagacagctt 2041 gcatgtcagc ctgccagcat ctgtcaaCAT CCTTAATGCA GATGCTACTG GACAGTGAGT 2101 TAAAACAAAT AAGCATGGGA GCTGTTCAGC AGTTTAACTT AGATGTCATA CAGTGTGAAT 2161 TGTTTGCCAG CTCTGAGCCT GTGCCAGGAT TCCAGGGGGA TACCCTGCAG CTAGCATTCA 2221 TTGACCTCAG ACAACTCCTT GACCTGTTTA TGGTTTGGGA TTGGTCTACT TACCTAGCTG 2281 ATTATGGGCA GCCAGCTTCT AAGTACCTTC GGGTGAATCC AAACACAGCC CTTACTCTTT 2341 TGGAGAAGAT GAAGGATACT AGCAAAAAGA ACAATATATT TGCTCAGTTC GGGAAGAATG 2401 ATCGAGACAA ACAGAAGTTG ATAGAGACAG TCGTGAAACA GCTGAGAAGT TTGGTGAATG 2461 GTATGTCCCA GCACATGTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2521 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2581 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACTAAGC TACCCAAAAT 2641 GATGTTTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt