Construct: ORF TRCN0000471946
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011946.1_s317c1
- Derived from:
- ccsbBroadEn_03364
- DNA Barcode:
- CCCCGAAGCGACCCATCTTTTGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASB2 (51676)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471946
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51676 | ASB2 | ankyrin repeat and SOCS box... | NM_016150.5 | 99.8% | 100% | 540T>C;1056C>G |
| 2 | human | 51676 | ASB2 | ankyrin repeat and SOCS box... | XM_017021369.2 | 90.9% | 89.6% | (many diffs) |
| 3 | human | 51676 | ASB2 | ankyrin repeat and SOCS box... | XM_011536835.3 | 90.5% | 90.4% | (many diffs) |
| 4 | human | 51676 | ASB2 | ankyrin repeat and SOCS box... | NM_001202429.2 | 90.3% | 88.6% | (many diffs) |
| 5 | human | 51676 | ASB2 | ankyrin repeat and SOCS box... | XM_005267758.3 | 90.3% | 88.6% | (many diffs) |
| 6 | human | 51676 | ASB2 | ankyrin repeat and SOCS box... | XM_011536834.3 | 82.2% | 80.5% | (many diffs) |
| 7 | mouse | 65256 | Asb2 | ankyrin repeat and SOCS box... | NM_023049.1 | 79.8% | 81.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1827
- ORF length:
- 1761
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ccgcttctcc tatgcagagt acttttccct ctttcactcc tgctctgcac 121 cctccaggtc cactgcacct cctgagagtt cgccggcccg ggccccaatg ggcttgttcc 181 aaggggtcat gcagaaatac agcagcagct tgttcaagac ctcccagctg gcgcctgcgg 241 accccttgat aaaggccatc aaggatggcg atgaagaggc cttgaagacc atgatcaagg 301 aagggaagaa tctcgcagag cccaacaagg agggctggct gccgctgcac gaggccgcat 361 actatggcca ggtgggctgc ctgaaagtcc tgcagcgagc gtacccaggg accatcgacc 421 agcgcaccct gcaggaggaa acagccgttt acttggcaac gtgcaggggc cacctggact 481 gtctcctgtc actgctccaa gcaggggcag agccggacat ctccaacaaa tcccgagaga 541 caccgctcta caaagcctgc gagcgcaaga acgcggaggc cgtgaagatt ctggtgcagc 601 acaacgcaga caccaaccac cgctgcaacc gcggctggac cgctctgcac gagtctgtgt 661 ctcgcaatga cctggaggtc atgcagatcc tggtgagcgg aggagccaag gtggaatcca 721 agaacgccta cggcatcacc cccttgttcg tggccgccca gagtggacag ttggaggcct 781 tgaggttctt agccaagtac ggtgctgaca tcaacacgca ggccagcgac aacgcgtctg 841 ccctctacga ggcctgcaag aatgagcatg aggaggtggt ggagtttctg ctgtcacagg 901 gtgccgacgc caacaagacc aacaaggacg gcttgctccc gctgcacatc gcctccaaga 961 agggcaacta caggatcgtg cagatgctgc tgccggtgac cagccgcacg cgcatacgcc 1021 gtagcggcgt cagtccgctg cacctggcgg ccgagcgcaa ccacgacgag gtgctggagg 1081 cgctgctgag cgcgcgcttc gacgtgaaca cgccgctggc gcccgagcgc gcgcgcctct 1141 acgaagaccg gcgcagctcc gcgctgtact tcgcggtggt caacaacaac gtgtacgcca 1201 ccgagctgct gctgcaacac ggcgccgacc ccaaccgcga cgtcatcagc cccttgctcg 1261 tggccatccg ccacggctgc ctgcgcacaa tgcagctgct gctggaccac ggcgcgaaca 1321 tcgacgccta tatcgccacg caccccaccg ccttccccgc caccatcatg ttcgccatga 1381 agtgcctgtc gctgctcaag ttcctcatgg acctgggctg cgacggcgag cccTGCTTCT 1441 CATGCCTCTA CGGCAACGGC CCGCACCCGC CGGCCCCGCA GCCCTCCAGC AGGTTCAACG 1501 ACGCGCCCGC GGCCGACAAG GAGCCCAGCG TGGTGCAGTT CTGTGAGTTC GTATCTGCCC 1561 CAGAGGTGAG CCGCTGGGCG GGGCCCATCA TCGATGTCCT CCTGGACTAC GTGGGCAACG 1621 TGCAGCTCTG CTCGCGGCTG AAGGAACACA TCGACAGCTT TGAGGACTGG GCCGTCATCA 1681 AGGAGAAGGC AGAACCTCCA AGACCTCTGG CTCACCTTTG CCGACTGCGG GTTCGAAAGG 1741 CCATTGGGAA ATACCGTATA AAACTCCTAG ACACCTTGCC GCTCCCAGGC AGGCTGATTA 1801 GATACCTGAA ATACGAGAAC ACCCAGTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 1861 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1921 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACCCCGAAG 1981 CGACCCATCT TTTGACACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2041 aagatt