Construct: ORF TRCN0000471947
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001814.1_s317c1
- Derived from:
- ccsbBroadEn_01270
- DNA Barcode:
- TCAGAATCTGCTTTGTGTCTCCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP6C (5537)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471947
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5537 | PPP6C | protein phosphatase 6 catal... | NM_002721.5 | 100% | 100% | |
| 2 | human | 5537 | PPP6C | protein phosphatase 6 catal... | XM_011518847.3 | 92.9% | 91.8% | (many diffs) |
| 3 | human | 5537 | PPP6C | protein phosphatase 6 catal... | NM_001123369.1 | 92.7% | 92.7% | 170_171ins66 |
| 4 | human | 5537 | PPP6C | protein phosphatase 6 catal... | NM_001123355.1 | 89.1% | 89.1% | 74_184del |
| 5 | human | 5537 | PPP6C | protein phosphatase 6 catal... | XM_024447607.1 | 51.8% | 51.8% | 0_1ins441 |
| 6 | mouse | 67857 | Ppp6c | protein phosphatase 6, cata... | NM_024209.2 | 93.6% | 99.6% | (many diffs) |
| 7 | mouse | 67857 | Ppp6c | protein phosphatase 6, cata... | XM_006498256.3 | 70.1% | 74.4% | (many diffs) |
| 8 | mouse | 67857 | Ppp6c | protein phosphatase 6, cata... | XM_017319227.1 | 63.9% | 63.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 981
- ORF length:
- 915
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gccgctagac ctggacaagt atgtggaaat agcgcggctg tgcaagtacc 121 tgccagagaa cgacctgaag cggctatgtg actacgtttg tgacctcctc ttagaagagt 181 caaatgttca gccagtatca acaccagtaa cagtgtgtgg agatatccat ggacagtttt 241 atgacctttg tgaactgttc agaactggag gtcaggttcc tgacacaaac tacatattta 301 tgggtgattt tgtagacaga ggttactata gtttggagac cttcacttac cttcttgcat 361 taaaggctaa atggcctgat cgtattacac ttttgcgagg aaatcatgag agtagacaga 421 taacacaggt ctatggattt tatgatgagt gccaaaccaa atatggaaat gctaatgcct 481 ggagatactg taccaaagtt tttgacatgc tcacagtagc agctttaata gatgagcaga 541 ttttgtgtgt ccatggtggt ttatctccTG ATATCAAAAC ACTGGATCAA ATTCGAACCA 601 TCGAACGGAA TCAGGAAATT CCTCATAAAG GAGCATTTTG TGATCTGGTT TGGTCAGATC 661 CTGAAGATGT GGATACCTGG GCTATCAGTC CCCGAGGAGC AGGTTGGCTT TTTGGAGCAA 721 AGGTCACAAA TGAGTTTGTT CATATCAACA ACTTAAAACT CATCTGCAGA GCACATCAAC 781 TAGTGCACGA AGGCTATAAA TTTATGTTTG ATGAGAAGCT GGTGACAGTA TGGTCTGCTC 841 CTAATTACTG CTATCGTTGT GGAAATATTG CTTCGATCAT GGTCTTCAAA GATGTAAATA 901 CAAGAGAACC AAAGTTATTC CGGGCAGTTC CAGATTCAGA ACGTGTTATT CCTCCCAGAA 961 CGACAACGCC ATATTTCCTT TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1021 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1081 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATCAG AATCTGCTTT 1141 GTGTCTCCAA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt