Construct: ORF TRCN0000471954
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018054.1_s317c1
- Derived from:
- ccsbBroadEn_07507
- DNA Barcode:
- TTATTTTCTAGTAGTGGCCCTGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SV2B (9899)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471954
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9899 | SV2B | synaptic vesicle glycoprote... | NM_001323031.2 | 99.9% | 100% | 279C>T |
| 2 | human | 9899 | SV2B | synaptic vesicle glycoprote... | NM_001323032.3 | 99.9% | 100% | 279C>T |
| 3 | human | 9899 | SV2B | synaptic vesicle glycoprote... | NM_001323037.3 | 99.9% | 100% | 279C>T |
| 4 | human | 9899 | SV2B | synaptic vesicle glycoprote... | NM_001323038.3 | 99.9% | 100% | 279C>T |
| 5 | human | 9899 | SV2B | synaptic vesicle glycoprote... | NM_001323039.3 | 99.9% | 100% | 279C>T |
| 6 | human | 9899 | SV2B | synaptic vesicle glycoprote... | NM_014848.7 | 99.9% | 100% | 279C>T |
| 7 | human | 9899 | SV2B | synaptic vesicle glycoprote... | XM_005254998.3 | 99.9% | 100% | 279C>T |
| 8 | human | 9899 | SV2B | synaptic vesicle glycoprote... | XM_017022761.1 | 99.9% | 100% | 279C>T |
| 9 | human | 9899 | SV2B | synaptic vesicle glycoprote... | XM_017022762.1 | 99.9% | 100% | 279C>T |
| 10 | human | 9899 | SV2B | synaptic vesicle glycoprote... | NM_001323034.3 | 90.1% | 90.1% | 279C>T;1506_1507ins201 |
| 11 | human | 9899 | SV2B | synaptic vesicle glycoprote... | NM_001323033.3 | 83.6% | 83.7% | 279C>T;450_451ins333 |
| 12 | human | 9899 | SV2B | synaptic vesicle glycoprote... | NM_001323036.3 | 78.8% | 78.3% | (many diffs) |
| 13 | human | 9899 | SV2B | synaptic vesicle glycoprote... | NM_001167580.3 | 77.8% | 77.8% | 0_1ins453 |
| 14 | human | 9899 | SV2B | synaptic vesicle glycoprote... | NM_001323040.3 | 77.8% | 77.8% | 0_1ins453 |
| 15 | mouse | 64176 | Sv2b | synaptic vesicle glycoprote... | NM_001109753.1 | 89.4% | 94.8% | (many diffs) |
| 16 | mouse | 64176 | Sv2b | synaptic vesicle glycoprote... | NM_153579.4 | 89.4% | 94.8% | (many diffs) |
| 17 | mouse | 64176 | Sv2b | synaptic vesicle glycoprote... | XM_006541059.3 | 89.4% | 94.8% | (many diffs) |
| 18 | mouse | 64176 | Sv2b | synaptic vesicle glycoprote... | XM_006541060.3 | 89.4% | 94.8% | (many diffs) |
| 19 | mouse | 64176 | Sv2b | synaptic vesicle glycoprote... | XM_006541061.2 | 89.4% | 94.8% | (many diffs) |
| 20 | mouse | 64176 | Sv2b | synaptic vesicle glycoprote... | XM_017312210.1 | 89.4% | 94.8% | (many diffs) |
| 21 | mouse | 64176 | Sv2b | synaptic vesicle glycoprote... | XM_017312212.1 | 89.4% | 94.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2115
- ORF length:
- 2049
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgactacaag tatcaggaca attatggggg ctatgctccc agtgatggct 121 attaccgcgg caatgagtcc aacccagaag aagatgcaca gagtgatgtc accgaaggcc 181 atgatgagga agacgagatc tatgagggcg agtaccaggg tatccctcac ccagatgatg 241 tcaaggccaa gcaggccaag atggcgccct ccagaatgga cagccttcgg ggccagacag 301 acctgatggc tgagaggctg gaagatgagg agcagttggc ccatcagtac gagaccatca 361 tggatgagtg tggccatggc cgcttccagt ggatcctctt tttcgtcttg ggtttggccc 421 tgatggccga tggggtggaa gtgttcgtgg tgagttttgc cctgcccagt gcagagaagg 481 acatgtgtct gtccagttcc aaaaaaggaa tgctagggat gatagtctac ttgggaatga 541 tggcgggcgc cttcatcctg ggaggcctgg ctgataagct gggaaggaag cgagtcctca 601 gcatgtctct ggccgtcaat gcctccttcg cctccctctc ttccttcgtg cagggatatg 661 gagccttcct cttctgccga ctcatctcag gcatcggtat tgggggtgct ctaccgattg 721 tttttgccta tttttctgaa ttcttgtctc gggagaagcg aggagaacac ctcagttggc 781 tgggcatctt ctggatgact gggggcctgt acgcatctgc catggcctgg agcatcatcc 841 cacactatgg ctggggcttc agcatgggga ccaattacca cttccatagc tggagagtgt 901 ttgtcatcgt ctgtgctctg ccctgcaccg tgtccatggt ggccctgaag ttcatgccag 961 agagcccaag gtttctgcta gagatgggca aacatgatga agcctggatg attctcaagc 1021 aagtccatga caccaacatg agagctaagg ggaccccaga gaaagtgttc acggtttcca 1081 acatcaaaac tcccaagcaa atggatgaat tcattgagat ccaaagttca acaggaacct 1141 ggtaccagcg ctggctggtc agattcaaga ccattttcaa gcaggtctgg gataatgccc 1201 tgtactgtgt gatggggccc tacagaatga atacactgat tctggccgtg gtttggtttg 1261 ccatggcatt cagttactat ggactgacag tttggtttcc tgatatgatc cgctattttc 1321 aagatgaaga atacaagtct aaaatgaagg tgttttttgg tgagcatgtg tacggcgcca 1381 caatcaactt cacgatggaa aatcagatcc accaacatgg gaaacttgtg aatgataagt 1441 tcacaagaat gtactttaaa catgtactct ttgaggacac attctttgac gagtgctatt 1501 ttgaagacgt aacatcaaca gatacctact tcaaaaattg taccattgaa tcaaccatct 1561 tttacaacac agacctctac gagcacaagt tcatcaactg tcggtttatc aactccacct 1621 tcctggagca gaaggagggc tgccacatgg acttggagca agataatgac ttcctgattt 1681 acctcgtcag cttcctgggc agcctgtctg tcttacccgg gaacatcatt tctgcccTGC 1741 TCATGGATAG AATTGGAAGG CTCAAGATGA TTGGTGGCTC CATGCTAATC TCTGCAGTCT 1801 GCTGCTTCTT CCTGTTTTTT GGCAACAGTG AGTCTGCAAT GATCGGCTGG CAGTGCCTGT 1861 TCTGTGGGAC AAGCATTGCA GCCTGGAATG CTCTGGATGT GATCACAGTG GAGCTGTATC 1921 CCACCAACCA GAGAGCAACA GCCTTCGGCA TTCTCAATGG ATTATGCAAA TTTGGCGCCA 1981 TCCTGGGAAA CACCATCTTT GCTTCTTTTG TTGGGATAAC CAAAGTGGTC CCCATCCTTC 2041 TGGCTGCTGC TTCTCTGGTT GGGGGTGGCC TGATTGCCCT TCGACTGCCA GAGACTCGAG 2101 AACAGGTCCT GATGAGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2161 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2221 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TTATTTTCTA GTAGTGGCCC 2281 TGGCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt