Construct: ORF TRCN0000471955
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018486.1_s317c1
- Derived from:
- ccsbBroadEn_01251
- DNA Barcode:
- CGGGGTTCAATCGCGGAGGTGCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPEF1 (5475)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471955
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5475 | PPEF1 | protein phosphatase with EF... | NM_006240.2 | 100% | 100% | |
| 2 | human | 5475 | PPEF1 | protein phosphatase with EF... | NM_152224.1 | 95.7% | 95.7% | 981_982ins84 |
| 3 | human | 5475 | PPEF1 | protein phosphatase with EF... | NM_152226.1 | 90.5% | 90.5% | 1063_1064ins186 |
| 4 | human | 5475 | PPEF1 | protein phosphatase with EF... | XM_017029612.1 | 86.2% | 86.2% | 0_1ins270 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2025
- ORF length:
- 1959
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg atgcagcagt tcttcaacga aaaccaggag atctgacaca tcactgagag 121 ctgcgttgat catccagaac tggtaccgag gttacaaagc tcgactgaag gccagacaac 181 actatgccct caccatcttc cagtccatcg aatatgctga tgaacaaggc caaatgcagt 241 tatccacctt cttttccttc atgttggaaa actacacaca tatacataag gaagagctag 301 aattaagaaa tcagtctctt gaaagcgaac aggacatgag ggatagatgg gattatgtgg 361 actcgataga tgtcccagac tcctataatg gtcctcggct acaatttcct ctcacttgta 421 cggatattga tttacttctt gaggccttca aggaacaaca gatacttcat gcccattatg 481 tcttagaggt gctatttgaa accaagaaag tcctgaagca aatgccgaat ttcactcaca 541 tacaaacttc tccctccaaa gaggtaacaa tctgtggtga tttgcatggg aaactggatg 601 atcttttttt gatcttctac aagaatggtc tcccctcaga gaggaacccg tatgttttta 661 atggtgactt tgtagatcga ggaaagaatt ccatagagat cctaatgatc ctgtgtgtga 721 gttttcttgt ctaccccaat gacctgcact tgaacagagg gaaccacgaa gattttatga 781 tgaatctgag gtatggcttc acgaaagaaa ttttgcataa atataagcta catggaaaaa 841 gaatcttaca aatcttggaa gaattctatg cctggctccc aatcggtaca atcgttgaca 901 atgaaatcct ggtcatccat ggtgggatat cagagaccac agacttgaat ttactccacc 961 gtgtagagag gaacaagatg aaatctgtgc tgataccacc aacggaaaca aacagagacc 1021 atgacactga ctcgaagcac aataaagtag gtgtgacttt taatgcacat ggaagaatca 1081 aaacaaatgg atctcctact gaacacttaa cagagcatga atgggaacag attattgata 1141 ttctgtggag tgatcccaga ggcaaaaatg gctgttttcc aaatacgtgc cgaggagggg 1201 gctgctattt tggaccagat gttacttcca agattcttaa taaataccag ttgaagatgc 1261 tcatcaggtc tcatgaatgt aagcccgaag ggtatgaaat ctgtcatgat gggaaggtgg 1321 tgactatatt ttctgcttct aattattatg aagaaggcag caatcgagga gcttacatca 1381 aactatgttc tggtacaact cctcgatttt tccagtacca agtaactaaa gcaacgtgct 1441 ttcagcctct tcgccaaaga gtggatacta tggaaaacag cgccatcaag atattaagag 1501 agagagtgat ttcacgaaaa agtgacctta ctcgtgcttt ccaacttcaa gaccacagaa 1561 aatcaggaaa actttctgtg agccagtggg ctttttgcat ggagaacatt ttggggctga 1621 acttaccatg gagatccctc agttcgaatc tggtaaacat agaccaaaat ggaaacgttg 1681 aatacatgtc cagcttccag aatatccgca ttgaaaaacc tGTACAAGAG GCTCATTCTA 1741 CTCTAGTTGA AACTCTGTAC AGATACAGAT CTGACCTGGA AATCATATTT AATGCCATTG 1801 ACACTGATCA CTCAGGCCTG ATCTCCGTGG AAGAATTTCG TGCCATGTGG AAACTTTTTA 1861 GTTCTCACTA CAATGTTCAC ATTGATGATT CCCAAGTCAA TAAGCTTGCC AACATAATGG 1921 ACTTGAACAA AGATGGAAGC ATTGACTTTA ATGAGTTTTT AAAGGCTTTC TATGTAGTGC 1981 ATAGATATGA AGACTTGATG AAACCTGATG TCACCAACCT TGGCTACCCA ACTTTCTTGT 2041 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2101 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2161 GAAAGGACGA CGGGGTTCAA TCGCGGAGGT GCTCACGCGT TAAGTCgaca atcaacctct 2221 ggattacaaa atttgtgaaa gatt