Transcript: Human NM_152224.1

Homo sapiens protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant 1b, mRNA.

Source:
NCBI, updated 2019-08-04
Taxon:
Homo sapiens (human)
Gene:
PPEF1 (5475)
Length:
2789
CDS:
482..2359

Additional Resources:

NCBI RefSeq record:
NM_152224.1
NBCI Gene record:
PPEF1 (5475)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_152224.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000002551 CGAGGAGCTTACATCAAACTA pLKO.1 1697 CDS 100% 5.625 7.875 N PPEF1 n/a
2 TRCN0000081222 GTCTTAGAGGTGCTATTTGAA pLKO.1 896 CDS 100% 5.625 7.875 N LOC436244 n/a
3 TRCN0000367462 AGATACTTCATGCCCATTATG pLKO_005 876 CDS 100% 13.200 10.560 N PPEF1 n/a
4 TRCN0000002550 GCTAGGCCCAAATCACAAGTA pLKO.1 2396 3UTR 100% 4.950 3.960 N PPEF1 n/a
5 TRCN0000356062 GATGTCCCAGACTCCTATAAT pLKO_005 785 CDS 100% 15.000 10.500 N PPEF1 n/a
6 TRCN0000367459 CAATAAGCTTGCCAACATAAT pLKO_005 2230 CDS 100% 13.200 9.240 N PPEF1 n/a
7 TRCN0000356063 CCTCTCACTTGTACGGATATT pLKO_005 824 CDS 100% 13.200 9.240 N PPEF1 n/a
8 TRCN0000002547 CGGCTACAATTTCCTCTCACT pLKO.1 812 CDS 100% 2.640 1.848 N PPEF1 n/a
9 TRCN0000002549 GTGACTTTGTAGATCGAGGAA pLKO.1 1080 CDS 100% 2.640 1.584 N PPEF1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_152224.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01251 pDONR223 100% 95.7% 95.7% None 981_982ins84 n/a
2 ccsbBroad304_01251 pLX_304 0% 95.7% 95.7% V5 981_982ins84 n/a
3 TRCN0000471955 CGGGGTTCAATCGCGGAGGTGCTC pLX_317 15.8% 95.7% 95.7% V5 981_982ins84 n/a
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