Construct: ORF TRCN0000471959
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014894.1_s317c1
- Derived from:
- ccsbBroadEn_03336
- DNA Barcode:
- TCGTCTAGCGGCAGCCCGTTTCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM49B (51571)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471959
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51571 | FAM49B | family with sequence simila... | NM_001256763.2 | 100% | 100% | |
| 2 | human | 51571 | FAM49B | family with sequence simila... | NM_001353242.2 | 100% | 100% | |
| 3 | human | 51571 | FAM49B | family with sequence simila... | NM_001353243.2 | 100% | 100% | |
| 4 | human | 51571 | FAM49B | family with sequence simila... | NM_001353244.2 | 100% | 100% | |
| 5 | human | 51571 | FAM49B | family with sequence simila... | NM_001353245.1 | 100% | 100% | |
| 6 | human | 51571 | FAM49B | family with sequence simila... | NM_001353246.1 | 100% | 100% | |
| 7 | human | 51571 | FAM49B | family with sequence simila... | NM_001353247.1 | 100% | 100% | |
| 8 | human | 51571 | FAM49B | family with sequence simila... | NM_001353248.2 | 100% | 100% | |
| 9 | human | 51571 | FAM49B | family with sequence simila... | NM_001353249.1 | 100% | 100% | |
| 10 | human | 51571 | FAM49B | family with sequence simila... | NM_001353250.1 | 100% | 100% | |
| 11 | human | 51571 | FAM49B | family with sequence simila... | NM_001353251.1 | 100% | 100% | |
| 12 | human | 51571 | FAM49B | family with sequence simila... | NM_001353252.1 | 100% | 100% | |
| 13 | human | 51571 | FAM49B | family with sequence simila... | NM_001353253.1 | 100% | 100% | |
| 14 | human | 51571 | FAM49B | family with sequence simila... | NM_001353254.2 | 100% | 100% | |
| 15 | human | 51571 | FAM49B | family with sequence simila... | NM_001353255.1 | 100% | 100% | |
| 16 | human | 51571 | FAM49B | family with sequence simila... | NM_001353256.2 | 100% | 100% | |
| 17 | human | 51571 | FAM49B | family with sequence simila... | NM_001353257.2 | 100% | 100% | |
| 18 | human | 51571 | FAM49B | family with sequence simila... | NM_001353258.2 | 100% | 100% | |
| 19 | human | 51571 | FAM49B | family with sequence simila... | NM_001353259.2 | 100% | 100% | |
| 20 | human | 51571 | FAM49B | family with sequence simila... | NM_001353260.2 | 100% | 100% | |
| 21 | human | 51571 | FAM49B | family with sequence simila... | NM_001353261.2 | 100% | 100% | |
| 22 | human | 51571 | FAM49B | family with sequence simila... | NM_001353262.2 | 100% | 100% | |
| 23 | human | 51571 | FAM49B | family with sequence simila... | NM_001353263.2 | 100% | 100% | |
| 24 | human | 51571 | FAM49B | family with sequence simila... | NM_001353264.2 | 100% | 100% | |
| 25 | human | 51571 | FAM49B | family with sequence simila... | NM_001353265.2 | 100% | 100% | |
| 26 | human | 51571 | FAM49B | family with sequence simila... | NM_001353266.2 | 100% | 100% | |
| 27 | human | 51571 | FAM49B | family with sequence simila... | NM_001353267.2 | 100% | 100% | |
| 28 | human | 51571 | FAM49B | family with sequence simila... | NM_001353268.2 | 100% | 100% | |
| 29 | human | 51571 | FAM49B | family with sequence simila... | NM_016623.5 | 100% | 100% | |
| 30 | human | 51571 | FAM49B | family with sequence simila... | XM_011517112.3 | 100% | 100% | |
| 31 | human | 51571 | FAM49B | family with sequence simila... | XM_011517121.3 | 100% | 100% | |
| 32 | human | 51571 | FAM49B | family with sequence simila... | XM_017013545.2 | 100% | 100% | |
| 33 | human | 51571 | FAM49B | family with sequence simila... | XM_017013548.1 | 100% | 100% | |
| 34 | human | 51571 | FAM49B | family with sequence simila... | XM_017013551.2 | 100% | 100% | |
| 35 | human | 51571 | FAM49B | family with sequence simila... | XM_024447180.1 | 100% | 100% | |
| 36 | human | 51571 | FAM49B | family with sequence simila... | NM_001353302.1 | 97% | 96.9% | (many diffs) |
| 37 | human | 51571 | FAM49B | family with sequence simila... | NM_001353303.1 | 97% | 96.9% | (many diffs) |
| 38 | human | 51571 | FAM49B | family with sequence simila... | NM_001353304.1 | 97% | 96.9% | (many diffs) |
| 39 | human | 51571 | FAM49B | family with sequence simila... | NM_001353305.1 | 97% | 96.9% | (many diffs) |
| 40 | human | 51571 | FAM49B | family with sequence simila... | NM_001353306.2 | 97% | 96.9% | (many diffs) |
| 41 | human | 51571 | FAM49B | family with sequence simila... | NM_001353307.2 | 97% | 96.9% | (many diffs) |
| 42 | human | 51571 | FAM49B | family with sequence simila... | NM_001353308.1 | 97% | 96.9% | (many diffs) |
| 43 | human | 51571 | FAM49B | family with sequence simila... | NM_001353309.1 | 97% | 96.9% | (many diffs) |
| 44 | human | 51571 | FAM49B | family with sequence simila... | NM_001353310.2 | 97% | 96.9% | (many diffs) |
| 45 | human | 51571 | FAM49B | family with sequence simila... | NM_001353311.2 | 97% | 96.9% | (many diffs) |
| 46 | human | 51571 | FAM49B | family with sequence simila... | NM_001353312.2 | 97% | 96.9% | (many diffs) |
| 47 | human | 51571 | FAM49B | family with sequence simila... | NM_001353313.2 | 97% | 96.9% | (many diffs) |
| 48 | human | 51571 | FAM49B | family with sequence simila... | NM_001353314.2 | 97% | 96.9% | (many diffs) |
| 49 | human | 51571 | FAM49B | family with sequence simila... | NM_001353315.2 | 97% | 96.9% | (many diffs) |
| 50 | human | 51571 | FAM49B | family with sequence simila... | XM_017013554.2 | 97% | 96.9% | (many diffs) |
| 51 | human | 51571 | FAM49B | family with sequence simila... | XM_017013557.2 | 97% | 96.9% | (many diffs) |
| 52 | human | 51571 | FAM49B | family with sequence simila... | NM_001353316.2 | 94.8% | 89.8% | (many diffs) |
| 53 | human | 51571 | FAM49B | family with sequence simila... | NM_001330612.2 | 54.9% | 54.9% | 0_1ins438 |
| 54 | human | 51571 | FAM49B | family with sequence simila... | NM_001353269.1 | 54.9% | 54.9% | 0_1ins438 |
| 55 | human | 51571 | FAM49B | family with sequence simila... | NM_001353270.1 | 54.9% | 54.9% | 0_1ins438 |
| 56 | human | 51571 | FAM49B | family with sequence simila... | NM_001353271.1 | 54.9% | 54.9% | 0_1ins438 |
| 57 | human | 51571 | FAM49B | family with sequence simila... | NM_001353272.1 | 54.9% | 54.9% | 0_1ins438 |
| 58 | human | 51571 | FAM49B | family with sequence simila... | NM_001353273.1 | 54.9% | 54.9% | 0_1ins438 |
| 59 | human | 51571 | FAM49B | family with sequence simila... | NM_001353274.1 | 54.9% | 54.9% | 0_1ins438 |
| 60 | human | 51571 | FAM49B | family with sequence simila... | NM_001353275.1 | 54.9% | 54.9% | 0_1ins438 |
| 61 | human | 51571 | FAM49B | family with sequence simila... | NM_001353276.2 | 54.9% | 54.9% | 0_1ins438 |
| 62 | human | 51571 | FAM49B | family with sequence simila... | NM_001353277.1 | 54.9% | 54.9% | 0_1ins438 |
| 63 | human | 51571 | FAM49B | family with sequence simila... | NM_001353278.1 | 54.9% | 54.9% | 0_1ins438 |
| 64 | human | 51571 | FAM49B | family with sequence simila... | NM_001353279.1 | 54.9% | 54.9% | 0_1ins438 |
| 65 | human | 51571 | FAM49B | family with sequence simila... | NM_001353280.1 | 54.9% | 54.9% | 0_1ins438 |
| 66 | human | 51571 | FAM49B | family with sequence simila... | NM_001353281.1 | 54.9% | 54.9% | 0_1ins438 |
| 67 | human | 51571 | FAM49B | family with sequence simila... | NM_001353282.2 | 54.9% | 54.9% | 0_1ins438 |
| 68 | human | 51571 | FAM49B | family with sequence simila... | NM_001353283.2 | 54.9% | 54.9% | 0_1ins438 |
| 69 | human | 51571 | FAM49B | family with sequence simila... | NM_001353284.2 | 54.9% | 54.9% | 0_1ins438 |
| 70 | human | 51571 | FAM49B | family with sequence simila... | NM_001353285.2 | 54.9% | 54.9% | 0_1ins438 |
| 71 | human | 51571 | FAM49B | family with sequence simila... | NM_001353286.2 | 54.9% | 54.9% | 0_1ins438 |
| 72 | human | 51571 | FAM49B | family with sequence simila... | NM_001353287.2 | 54.9% | 54.9% | 0_1ins438 |
| 73 | human | 51571 | FAM49B | family with sequence simila... | NM_001353288.2 | 54.9% | 54.9% | 0_1ins438 |
| 74 | human | 51571 | FAM49B | family with sequence simila... | NM_001353289.2 | 54.9% | 54.9% | 0_1ins438 |
| 75 | human | 51571 | FAM49B | family with sequence simila... | NM_001353290.2 | 54.9% | 54.9% | 0_1ins438 |
| 76 | human | 51571 | FAM49B | family with sequence simila... | NM_001353291.2 | 54.9% | 54.9% | 0_1ins438 |
| 77 | human | 51571 | FAM49B | family with sequence simila... | NM_001353292.2 | 54.9% | 54.9% | 0_1ins438 |
| 78 | human | 51571 | FAM49B | family with sequence simila... | NM_001353293.2 | 54.9% | 54.9% | 0_1ins438 |
| 79 | human | 51571 | FAM49B | family with sequence simila... | NM_001353294.2 | 54.9% | 54.9% | 0_1ins438 |
| 80 | human | 51571 | FAM49B | family with sequence simila... | NM_001353295.2 | 54.9% | 54.9% | 0_1ins438 |
| 81 | human | 51571 | FAM49B | family with sequence simila... | NM_001353296.2 | 54.9% | 54.9% | 0_1ins438 |
| 82 | human | 51571 | FAM49B | family with sequence simila... | NM_001353297.2 | 54.9% | 54.9% | 0_1ins438 |
| 83 | human | 51571 | FAM49B | family with sequence simila... | NM_001353298.2 | 54.9% | 54.9% | 0_1ins438 |
| 84 | human | 51571 | FAM49B | family with sequence simila... | NM_001353299.2 | 54.9% | 54.9% | 0_1ins438 |
| 85 | human | 51571 | FAM49B | family with sequence simila... | NM_001353300.2 | 54.9% | 54.9% | 0_1ins438 |
| 86 | human | 51571 | FAM49B | family with sequence simila... | NM_001353301.2 | 54.9% | 54.9% | 0_1ins438 |
| 87 | mouse | 223601 | Fam49b | family with sequence simila... | NM_144846.5 | 94.7% | 99.6% | (many diffs) |
| 88 | mouse | 223601 | Fam49b | family with sequence simila... | XM_006520761.2 | 94.7% | 99.6% | (many diffs) |
| 89 | mouse | 223601 | Fam49b | family with sequence simila... | XM_006520763.2 | 94.7% | 99.6% | (many diffs) |
| 90 | mouse | 223601 | Fam49b | family with sequence simila... | XM_006520764.3 | 94.7% | 99.6% | (many diffs) |
| 91 | mouse | 223601 | Fam49b | family with sequence simila... | XM_011245563.1 | 94.7% | 99.6% | (many diffs) |
| 92 | mouse | 223601 | Fam49b | family with sequence simila... | XM_011245565.2 | 94.7% | 99.6% | (many diffs) |
| 93 | mouse | 223601 | Fam49b | family with sequence simila... | XM_006520762.3 | 91.8% | 96.6% | (many diffs) |
| 94 | mouse | 223601 | Fam49b | family with sequence simila... | XM_006520765.2 | 91.8% | 96.6% | (many diffs) |
| 95 | mouse | 223601 | Fam49b | family with sequence simila... | XM_011245564.1 | 91.8% | 96.6% | (many diffs) |
| 96 | mouse | 223601 | Fam49b | family with sequence simila... | XM_017316550.1 | 91.8% | 96.6% | (many diffs) |
| 97 | mouse | 223601 | Fam49b | family with sequence simila... | XM_006520766.2 | 51.9% | 54.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1038
- ORF length:
- 972
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gaatcttctt aaagttttga catgcacaga ccttgagcag gggccaaatt 121 ttttccttga ttttgaaaat gcccagccta cagagtctga gaaggaaatt tataatcagg 181 tgaatgtagt attaaaagat gcagaaggca tcttggagga cttgcagtca tacagaggag 241 ctggccacga aatacgagag gcaatccagc atccagcaga tgagaagttg caagagaagg 301 catggggtgc agttgttcca ctagtaggca aattaaagaa attttacgaa ttttctcaga 361 ggttagaagc agcattaaga ggtcttctgg gagccttaac aagtacccca tattctccca 421 cccagcatct agagcgagag caggctcttg ctaaacagtt tgcagaaatt cttcatttca 481 cactccggtt tgatgaactc aagatgacaa atcctgccat acagaatgat ttcagctatt 541 atagaagaac attgagtcgt atgaggatta acaatgtacc ggcagaagga gaaaatgaag 601 taaataatga attggcaaat cgaatgtctt tgttttatgc tgaggcaact ccaatgctga 661 aaacctTGAG TGATGCCACA ACAAAATTTG TATCAGAGAA TAAAAATTTA CCAATAGAAA 721 ATACCACAGA TTGTTTAAGC ACAATGGCTA GTGTATGCAG AGTCATGCTG GAAACACCGG 781 AATACAGAAG CAGATTTACA AATGAAGAGA CAGTGTCATT CTGCTTGAGG GTAATGGTGG 841 GTGTCATAAT ACTCTATGAC CACGTACATC CAGTGGGAGC ATTTGCTAAA ACTTCCAAAA 901 TTGATATGAA AGGTTGTATC AAAGTTCTTA AGGACCAACC TCCTAATAGT GTGGAAGGTC 961 TTCTAAATGC TCTCAGGTAC ACAACAAAAC ATTTGAATGA TGAGACTACC TCCAAGCAAA 1021 TTAAATCCAT GCTGCAATAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1081 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1141 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATCGTCTA GCGGCAGCCC 1201 GTTTCCCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt