Construct: ORF TRCN0000471976
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003666.1_s317c1
- Derived from:
- ccsbBroadEn_03119
- DNA Barcode:
- GTCTTGGCCGAGATCGTTTGCTGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CDIP1 (29965)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471976
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29965 | CDIP1 | cell death inducing p53 tar... | NM_001199054.2 | 100% | 100% | |
| 2 | human | 29965 | CDIP1 | cell death inducing p53 tar... | NM_013399.3 | 100% | 100% | |
| 3 | human | 29965 | CDIP1 | cell death inducing p53 tar... | NM_001199055.2 | 81.2% | 81.2% | 240_241ins117 |
| 4 | human | 29965 | CDIP1 | cell death inducing p53 tar... | NM_001199056.2 | 62% | 62% | 118_119ins237 |
| 5 | mouse | 66626 | Cdip1 | cell death inducing Trp53 t... | NM_025670.4 | 88.1% | 94.2% | (many diffs) |
| 6 | mouse | 66626 | Cdip1 | cell death inducing Trp53 t... | XM_006522467.1 | 88.1% | 94.2% | (many diffs) |
| 7 | mouse | 66626 | Cdip1 | cell death inducing Trp53 t... | XM_006522468.1 | 88.1% | 94.2% | (many diffs) |
| 8 | mouse | 66626 | Cdip1 | cell death inducing Trp53 t... | XM_011245996.2 | 88.1% | 94.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 690
- ORF length:
- 624
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cagcgagcct ccccctcctt atcctggggg ccccacagcc ccacttctgg 121 aagagaaaag tggagccccg cccaccccag gccgttcctc cccagctgtg atgcagcccc 181 ctccaggcat gccactgccc cctgcggaca ttggcccccc accctatgag ccgccgggtc 241 acccaatgcc ccagcctggc ttcatcccac cacacatgag tgcagatggc acctacatgc 301 cTCCGGGTTT CTACCCTCCT CCAGGCCCCC ACCCACCCAT GGGCTACTAC CCCCCAGGGC 361 CCTACACGCC AGGGCCCTAC CCTGGCCCTG GGGGCCACAC AGCCACAGTC CTGGTCCCTT 421 CAGGAGCTGC CACCACGGTG ACAGTGCTGC AGGGAGAGAT CTTTGAGGGA GCGCCTGTGC 481 AGACGGTGTG TCCCCACTGC CAGCAGGCCA TCACCACCAA GATCTCCTAC GAGATTGGCT 541 TGATGAATTT CGTGCTGGGT TTCTTCTGTT GCTTCATGGG ATGTGATCTG GGCTGCTGCC 601 TGATCCCCTG CCTCATCAAT GACTTCAAGG ATGTGACGCA CACATGCCCC AGCTGCAAAG 661 CCTACATCTA CACGTACAAG CGCCTGTGCT AGCCAACTTT CTTGTACAAA GTGGTTGATA 721 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 781 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGTCTT 841 GGCCGAGATC GTTTGCTGCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 901 tgaaagatt