Construct: ORF TRCN0000471985

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005763.1_s317c1
Derived from:
ccsbBroadEn_02251
DNA Barcode:
CCTTGGGCCGGACTCTACTATTCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NUP58 (9818)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471985
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9818 NUP58 nucleoporin 58 NM_014089.4 100% 100%
2 human 9818 NUP58 nucleoporin 58 NM_001008564.2 97.9% 97.9% 249_250ins36
3 human 9818 NUP58 nucleoporin 58 XM_005266600.4 94.4% 94.4% 1433_1434ins99
4 human 9818 NUP58 nucleoporin 58 XM_011535328.2 93.3% 91.8% (many diffs)
5 human 9818 NUP58 nucleoporin 58 XM_011535329.3 54.2% 54.2% 0_1ins822
6 human 9818 NUP58 nucleoporin 58 XM_017020881.2 54.2% 54.2% 0_1ins822
7 mouse 71844 Nupl1 nucleoporin like 1 NM_170591.1 88.7% 90.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1863
ORF length:
1797
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc cacagggttc tccttcgggt ccgggactct gggctccacc accgtggccg
121ccggcgggac cagcacaggc ggcgttttct ccttcggaac gggagcgtct agcaaccctt
181ctgtggggct caattttgga aatcttggaa gtacttcaac tccagcaact acatctgctc
241cttcaagtgg ttttggaacc gggctctttg gatctaaacc tgccactggg ttcactctag
301gaggaacaaa tacaggaata gcaacaacta taactacagg attaactctg ggaacgccag
361ccactacatc tgcagctaca acaggcttca gtttaggatt caataaacct gcagcatctg
421ccacaccatt tgctctacct attacctcta cctcagctag cggtctgact ctttcgtctg
481ctctgacatc aactccagca gcatccacag gatttactct aaataatttg ggtgggacaa
541cagccacaac tacaactgca tcaacaggcc tctctttagg gggagcctta gctggtttgg
601gaggttcact tttccagagt acaaacacag gaacatcagg acttggacag aatgctttag
661ggttgacttt gggaactaca gcagctactt caactgcagg caatgaaggc cttggtggta
721tagatttcag tagctcctca gataaaaaga gtgataaaac gggaacaaga ccagaggata
781gtaaagctct gaaggatgaa aatctacctc ctgtcatctg ccaggatgtt gaaaatctcc
841agaaatttgt gaaggagcag aaacaagttc aagaagaaat tagtagaatg tcttcaaaag
901caatgcttaa ggtacaagaa gatattaaag ctctgaagca gctcctgtcg ttggctgcca
961atggaataca gagaaacact ctcaacattg acaaattgaa aatagaaact gctcaggagt
1021tgaagaatgc tgaaatagct ttaagaaccc agaagacacc acctggactt caacatgaat
1081atgcagctcc tgctgactac ttcagaatct tggttcagca atttgaggta cagcttcagc
1141agtacaggca gcagattgaa gaactagaaa accatcttgc cactcaagca aataattcac
1201atataacccc tcaagatttg tcaatggcta tgcagaaaat ttatcaaaca tttgtagctt
1261tagcggcaca acttcagtct attcatgaaa atgtaaaggt tctgaaagaa cagtaccttg
1321gctacaggaa aatgttcttg ggagatgctg ttgatgtgtt tgaaacaagg cgagcagaag
1381ccaagaagtg gcagaacaca cccagagtta ctactggacc cactcctttc agcaccatgc
1441caaacgcagc agccgttgcc atggctgcaa cacttacaca gcagcaacag cctgctacag
1501ggccacagcc atctctgGGA GTTAGTTTTG GAACGCCATT CGGCTCAGGT ATTGGCACTG
1561GCTTGCAATC AAGTGGCTTA GGTTCTTCAA ACCTTGGAGG ATTTGGAACT AGCTCTGGTT
1621TTGGATGCAG CACCACAGGG GCCTCCACAT TTGGATTTGG AACAACAAAT AAACCCTCAG
1681GAAGTCTTAG TGCAGGCTTT GGCAGCTCAA GTACATCTGG GTTTAACTTC AGCAATCCTG
1741GCATCACGGC ATCAGCTGGT TTGACTTTTG GGGTGTCCAA TCCTGCCTCT GCAGGTTTTG
1801GAACAGGAGG ACAACTCCTT CAGTTGAAGA AACCTCCAGC TGGAAACAAA AGAGGAAAAA
1861GATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
1921TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
1981GGCTTTATAT ATCTTGTGGA AAGGACGACC TTGGGCCGGA CTCTACTATT CTACGCGTTA
2041AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)