Construct: ORF TRCN0000471985
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005763.1_s317c1
- Derived from:
- ccsbBroadEn_02251
- DNA Barcode:
- CCTTGGGCCGGACTCTACTATTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUP58 (9818)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471985
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9818 | NUP58 | nucleoporin 58 | NM_014089.4 | 100% | 100% | |
| 2 | human | 9818 | NUP58 | nucleoporin 58 | NM_001008564.2 | 97.9% | 97.9% | 249_250ins36 |
| 3 | human | 9818 | NUP58 | nucleoporin 58 | XM_005266600.4 | 94.4% | 94.4% | 1433_1434ins99 |
| 4 | human | 9818 | NUP58 | nucleoporin 58 | XM_011535328.2 | 93.3% | 91.8% | (many diffs) |
| 5 | human | 9818 | NUP58 | nucleoporin 58 | XM_011535329.3 | 54.2% | 54.2% | 0_1ins822 |
| 6 | human | 9818 | NUP58 | nucleoporin 58 | XM_017020881.2 | 54.2% | 54.2% | 0_1ins822 |
| 7 | mouse | 71844 | Nupl1 | nucleoporin like 1 | NM_170591.1 | 88.7% | 90.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1863
- ORF length:
- 1797
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cacagggttc tccttcgggt ccgggactct gggctccacc accgtggccg 121 ccggcgggac cagcacaggc ggcgttttct ccttcggaac gggagcgtct agcaaccctt 181 ctgtggggct caattttgga aatcttggaa gtacttcaac tccagcaact acatctgctc 241 cttcaagtgg ttttggaacc gggctctttg gatctaaacc tgccactggg ttcactctag 301 gaggaacaaa tacaggaata gcaacaacta taactacagg attaactctg ggaacgccag 361 ccactacatc tgcagctaca acaggcttca gtttaggatt caataaacct gcagcatctg 421 ccacaccatt tgctctacct attacctcta cctcagctag cggtctgact ctttcgtctg 481 ctctgacatc aactccagca gcatccacag gatttactct aaataatttg ggtgggacaa 541 cagccacaac tacaactgca tcaacaggcc tctctttagg gggagcctta gctggtttgg 601 gaggttcact tttccagagt acaaacacag gaacatcagg acttggacag aatgctttag 661 ggttgacttt gggaactaca gcagctactt caactgcagg caatgaaggc cttggtggta 721 tagatttcag tagctcctca gataaaaaga gtgataaaac gggaacaaga ccagaggata 781 gtaaagctct gaaggatgaa aatctacctc ctgtcatctg ccaggatgtt gaaaatctcc 841 agaaatttgt gaaggagcag aaacaagttc aagaagaaat tagtagaatg tcttcaaaag 901 caatgcttaa ggtacaagaa gatattaaag ctctgaagca gctcctgtcg ttggctgcca 961 atggaataca gagaaacact ctcaacattg acaaattgaa aatagaaact gctcaggagt 1021 tgaagaatgc tgaaatagct ttaagaaccc agaagacacc acctggactt caacatgaat 1081 atgcagctcc tgctgactac ttcagaatct tggttcagca atttgaggta cagcttcagc 1141 agtacaggca gcagattgaa gaactagaaa accatcttgc cactcaagca aataattcac 1201 atataacccc tcaagatttg tcaatggcta tgcagaaaat ttatcaaaca tttgtagctt 1261 tagcggcaca acttcagtct attcatgaaa atgtaaaggt tctgaaagaa cagtaccttg 1321 gctacaggaa aatgttcttg ggagatgctg ttgatgtgtt tgaaacaagg cgagcagaag 1381 ccaagaagtg gcagaacaca cccagagtta ctactggacc cactcctttc agcaccatgc 1441 caaacgcagc agccgttgcc atggctgcaa cacttacaca gcagcaacag cctgctacag 1501 ggccacagcc atctctgGGA GTTAGTTTTG GAACGCCATT CGGCTCAGGT ATTGGCACTG 1561 GCTTGCAATC AAGTGGCTTA GGTTCTTCAA ACCTTGGAGG ATTTGGAACT AGCTCTGGTT 1621 TTGGATGCAG CACCACAGGG GCCTCCACAT TTGGATTTGG AACAACAAAT AAACCCTCAG 1681 GAAGTCTTAG TGCAGGCTTT GGCAGCTCAA GTACATCTGG GTTTAACTTC AGCAATCCTG 1741 GCATCACGGC ATCAGCTGGT TTGACTTTTG GGGTGTCCAA TCCTGCCTCT GCAGGTTTTG 1801 GAACAGGAGG ACAACTCCTT CAGTTGAAGA AACCTCCAGC TGGAAACAAA AGAGGAAAAA 1861 GATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1921 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1981 GGCTTTATAT ATCTTGTGGA AAGGACGACC TTGGGCCGGA CTCTACTATT CTACGCGTTA 2041 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt