Transcript: Human NM_014089.4

Homo sapiens nucleoporin 58 (NUP58), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
NUP58 (9818)
Length:
4236
CDS:
150..1949

Additional Resources:

NCBI RefSeq record:
NM_014089.4
NBCI Gene record:
NUP58 (9818)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_014089.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000013517 GCGGCACAACTTCAGTCTATT pLKO.1 1347 CDS 100% 13.200 10.560 N NUP58 n/a
2 TRCN0000297841 GCGGCACAACTTCAGTCTATT pLKO_005 1347 CDS 100% 13.200 10.560 N NUP58 n/a
3 TRCN0000013514 CCACAGGATTTACTCTAAATA pLKO.1 589 CDS 100% 15.000 10.500 N NUP58 n/a
4 TRCN0000280443 CCACAGGATTTACTCTAAATA pLKO_005 589 CDS 100% 15.000 10.500 N NUP58 n/a
5 TRCN0000013516 GCTTCAGTTTAGGATTCAATA pLKO.1 469 CDS 100% 13.200 9.240 N NUP58 n/a
6 TRCN0000280396 GCTTCAGTTTAGGATTCAATA pLKO_005 469 CDS 100% 13.200 9.240 N NUP58 n/a
7 TRCN0000013513 CCGTTCATTCTATGAGTCTAT pLKO.1 1986 3UTR 100% 4.950 3.465 N NUP58 n/a
8 TRCN0000280398 CCGTTCATTCTATGAGTCTAT pLKO_005 1986 3UTR 100% 4.950 3.465 N NUP58 n/a
9 TRCN0000013515 CCTCCACATTTGGATTTGGAA pLKO.1 1726 CDS 100% 3.000 2.100 N NUP58 n/a
10 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 2474 3UTR 100% 4.950 2.475 Y ERAP2 n/a
11 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 2475 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_014089.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02251 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_02251 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000471985 CCTTGGGCCGGACTCTACTATTCT pLX_317 19.5% 100% 100% V5 n/a
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