Construct: ORF TRCN0000471988
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000709.1_s317c1
- Derived from:
- ccsbBroadEn_12034
- DNA Barcode:
- ATCCACCCTGGGGACTCACCTCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNTG2 (54221)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471988
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54221 | SNTG2 | syntrophin gamma 2 | XM_011510366.2 | 84.5% | 75.2% | (many diffs) |
| 2 | human | 54221 | SNTG2 | syntrophin gamma 2 | XM_017004367.1 | 84.5% | 75.2% | (many diffs) |
| 3 | human | 54221 | SNTG2 | syntrophin gamma 2 | XM_017004364.1 | 80.8% | 69.2% | (many diffs) |
| 4 | human | 54221 | SNTG2 | syntrophin gamma 2 | XM_017004363.1 | 80% | 67.3% | (many diffs) |
| 5 | human | 54221 | SNTG2 | syntrophin gamma 2 | NM_018968.4 | 76.3% | 76.2% | 189C>T;211_591del;1484C>T |
| 6 | human | 54221 | SNTG2 | syntrophin gamma 2 | XM_017004373.2 | 76.1% | 75.9% | 0_1ins294;809C>T |
| 7 | human | 54221 | SNTG2 | syntrophin gamma 2 | XM_024452970.1 | 76.1% | 75.9% | 0_1ins294;809C>T |
| 8 | human | 54221 | SNTG2 | syntrophin gamma 2 | XM_017004361.1 | 72.6% | 72.2% | (many diffs) |
| 9 | human | 54221 | SNTG2 | syntrophin gamma 2 | XM_017004362.1 | 63.6% | 58.3% | (many diffs) |
| 10 | human | 54221 | SNTG2 | syntrophin gamma 2 | XM_017004366.1 | 53.4% | 51.6% | (many diffs) |
| 11 | human | 54221 | SNTG2 | syntrophin gamma 2 | XM_017004365.1 | 52.7% | 51.8% | (many diffs) |
| 12 | human | 54221 | SNTG2 | syntrophin gamma 2 | XM_017004368.1 | 37.5% | 35.1% | (many diffs) |
| 13 | human | 54221 | SNTG2 | syntrophin gamma 2 | XM_017004369.1 | 36.9% | 34.6% | (many diffs) |
| 14 | human | 54221 | SNTG2 | syntrophin gamma 2 | XM_017004370.1 | 35.7% | 34.6% | (many diffs) |
| 15 | human | 54221 | SNTG2 | syntrophin gamma 2 | XM_017004371.1 | 30.9% | 28.3% | (many diffs) |
| 16 | human | 54221 | SNTG2 | syntrophin gamma 2 | XM_017004372.1 | 30.9% | 28.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1305
- ORF length:
- 1236
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcaccgag ggacccccgc ccccggccgc ctcccgcgga cgccagggct 121 gcctgctggt acctgcgcgg acgaaaacca ctattgctct gttgtatgat gaagagtccg 181 aaaatgccta tgacatccgg ctgaagctga cgaaagaggt gctgacaatt cagaaacaag 241 atgttgtctg tgtgggtgga agccaccagg gcaggaatgc cccatcgtca ccttcctcgc 301 ccatagctaa ggacccgagg tatgagaagc gctggctgga caccttgtcc gtgcctctgt 361 ccatggctcg catctcaagg tacaaagccg gaacggaaaa attaaggtgg aatgcgttcg 421 aggtgctcgc cctggacgga gtcagctctg ggatcctccg gttttacaca gcccaggatg 481 gcaccgactg gctgcgggcg gtctcagcca acatcaggga gctgacactt cagaacatga 541 agatggcgaa caaatgctgc tctccttccg accaggttgt gcatatgggg tgggtaaatg 601 agaaactcca aggagctgac tcctctcaaa ccttcagacc caagttccta gcactgaagg 661 gcccgtcctt ctacgttttc agcactcctc cggtgagcac attcgattgg gtgcgagcag 721 aaaggaccta tcacctctgt gaggtgctat ttaaagttca caagttctgg ctcacagagg 781 actgctggtt gcaagcaaac ttgtatctgg gtcttcaaga ttttgacttt gaggaccaga 841 ggccctattg cttcagcatc gtggccggcc atgggaagag ccatgttttc aacgtggagc 901 ttggcagcga gctggccatg tgggagaagt ccttccaaag agccacgttc atggaagttc 961 agagaaccgg gtccagaaca tacatgtgca gctggcaagg agagatgctg tgtttcacgg 1021 tggatttcgc gttgggattt acctgttttg agagtaagac caagaatgtg ctctggagat 1081 ttaaattttc ccagcttaag ggatcttcag atgatgggaa aactcgagta aagctgctgt 1141 ttcagaatct ggacaccaaa cagattgaga tgaaggaact cgagttccag gaccTGAGGG 1201 CTGTCCTGCA CTGCATCCAC TCCTTCATAG CAGCCAAGGT GGCCTCCGTG GACCCCGGCT 1261 TCATGGACAG TCAGAGTCTT GCCAGAAAAT ACATGTACAG CAGCTTGCCA ACTTTCTTGT 1321 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1381 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1441 GAAAGGACGA ATCCACCCTG GGGACTCACC TCAGACGCGT TAAGTCgaca atcaacctct 1501 ggattacaaa atttgtgaaa gatt