Construct: ORF TRCN0000471989
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004255.1_s317c1
- Derived from:
- ccsbBroadEn_06976
- DNA Barcode:
- GGATACCGGTCGTCCGGTGAAGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMARCE1 (6605)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471989
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6605 | SMARCE1 | SWI/SNF related, matrix ass... | NM_003079.5 | 99.9% | 99.7% | 136G>A |
| 2 | mouse | 57376 | Smarce1 | SWI/SNF related, matrix ass... | NM_020618.4 | 92.3% | 97% | (many diffs) |
| 3 | mouse | 57376 | Smarce1 | SWI/SNF related, matrix ass... | XM_006533827.3 | 83.8% | 88.8% | (many diffs) |
| 4 | mouse | 57376 | Smarce1 | SWI/SNF related, matrix ass... | XM_006533828.2 | 75.7% | 80.2% | (many diffs) |
| 5 | mouse | 57376 | Smarce1 | SWI/SNF related, matrix ass... | XM_011249158.1 | 75.7% | 80.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1299
- ORF length:
- 1233
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc aaaaagacca tcttatgccc cacctcccac cccagctcct gcaacacaaa 121 tgcccagcac accagggttt gtgggataca atccatacag tcatctcgcc tacaacaact 181 acaggctggg agggaacccg agcaccaaca gccgggtcac ggcatcctct ggtatcacga 241 ttccaaaacc cccaaagcca ccagataagc cgctgatgcc ctacatgagg tacagcagaa 301 aggtctggga ccaagtaaag gcttccaacc ctgacctaaa gttgtgggag attggcaaga 361 ttattggtgg catgtggcga gatctcactg atgaagaaaa acaagaatat ttaaacgaat 421 acgaagcaga aaagatagag tacaatgaat ctatgaaggc ctatcataat tcccccgcgt 481 accttgctta cataaatgca aaaagtcgtg cagaagctgc tttagaggaa gaaagtcgac 541 agagacaatc tcgcatggag aaaggagaac cgtacatgag cattcagcct gctgaagatc 601 cagatgatta tgatgatggc ttttcaatga agcatacagc caccgcccgt ttccagagaa 661 accaccgcct catcagtgaa attcttagtg agagtgtggt gccagacgtt cggtcagttg 721 tcacaacagc tagaatgcag gtcctcaaac ggcaggtcca gtccttaatg gttcatcagc 781 gaaaactaga agctgaactt cttcaaatag aggaacgaca ccaggagaag aagaggaaat 841 tcctggaaag cacagattca tttaacaatg aacttaaaag gttgtgcggt ctgaaagtag 901 aagtggatat ggagaaaatt gcagctgaga ttgcacaggc agaggaacag gcccgcaaaa 961 ggcaggagga aagggagaag gaggccgcag agcaagctga gcgcagtcag agcagcatcg 1021 ttcctgagga agaacaagca gctaacaaag gcgaggagaa gaaagacgac gagaacattc 1081 cgatggagac agaggagaca caccttgaag aaacaacaga gagccaacag aatggTGAAG 1141 AAGGCACGTC TACTCCTGAG GACAAGGAGA GTGGGCAGGA GGGGGTCGAC AGTATGGCAG 1201 AGGAAGGAAC CAGTGATAGT AACACTGGCT CGGAGAGCAA CAGTGCAACA GTGGAGGAGC 1261 CACCAACAGA TCCCATACCA GAAGATGAGA AAAAAGAATA CCCAACTTTC TTGTACAAAG 1321 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1381 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1441 ACGAGGATAC CGGTCGTCCG GTGAAGTGAC GCGTTAAGTC gacaatcaac ctctggatta 1501 caaaatttgt gaaagatt