Construct: ORF TRCN0000472008
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014434.1_s317c1
- Derived from:
- ccsbBroadEn_01314
- DNA Barcode:
- CAGCATATTCAAAGCTATCCCTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSMC2 (5701)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472008
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5701 | PSMC2 | proteasome 26S subunit, ATP... | NM_002803.4 | 99.9% | 100% | 934A>C |
| 2 | human | 5701 | PSMC2 | proteasome 26S subunit, ATP... | XR_002956469.1 | 37.2% | (many diffs) | |
| 3 | human | 5701 | PSMC2 | proteasome 26S subunit, ATP... | XR_002956468.1 | 36% | 1_309del;731_1327del;1702_1703ins503 | |
| 4 | human | 5701 | PSMC2 | proteasome 26S subunit, ATP... | NM_001204453.1 | 32.4% | 32.5% | 423_423delGins877 |
| 5 | mouse | 19181 | Psmc2 | proteasome (prosome, macrop... | NM_011188.3 | 80.9% | 90.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1365
- ORF length:
- 1299
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ggattacctc ggtgccgatc agcggaagac caaagaggat gagaaggacg 121 acaagcccat ccgagctctg gatgaggggg atattgcctt gttgaaaact tatggtcaga 181 gcacttactc taggcagatc aagcaagttg aagatgacat tcagcaactt ctcaagaaaa 241 ttaatgagct cactggtatt aaagaatctg acactggcct ggccccacca gcactctggg 301 atttggctgc agataagcag acactccaga gtgaacagcc tttacaggtt gccaggtgta 361 caaagataat caatgctgat tcggaggacc caaaatacat tatcaacgta aagcagtttg 421 ccaagtttgt ggtggacctt agtgatcagg tggcacctac tgacattgaa gaagggatga 481 gagtgggcgt ggatagaaat aaatatcaaa ttcacattcc attgcctcct aagattgacc 541 caacagttac catgatgcag gtggaagaga aacctgatgt cacatacagt gatgttggtg 601 gctgtaagga acagattgag aaactgcgag aagtagttga aaccccatta cttcatccag 661 agaggtttgt gaaccttggc attgagcctc ccaagggcgt gctgctcttt ggtccacccg 721 gtacaggcaa gacactctgt gcgcgggcag ttgctaatcg gactgatgcg tgcttcattc 781 gagttattgg atctgagctt gtacagaaat acgtcggtga gggggctcga atggttcgtg 841 aactctttga aatggccaga acaaaaaaag cctgccTTAT CTTCTTTGAT GAAATTGATG 901 CTATTGGAGG GGCTCGTTTT GATGATGGTG CTGGAGGTGA CAATGAAGTG CAGAGAACAA 961 TGTTGGAACT GATCAATCAG CTTGATGGTT TTGATCCTCG AGGCAATATT AAAGTGCTGA 1021 TGGCCACTAA CAGACCTGAT ACTTTGGATC CAGCACTGAT GAGGCCAGGG AGATTGGATA 1081 GAAAAATTGA ATTTAGCTTG CCCGATCTAG AGGGTCGGAC CCACATATTT AAGATTCACG 1141 CTCGTTCAAT GAGTGTTGAA AGAGATATCA GATTTGAACT GTTAGCACGA CTGTGTCCAA 1201 ATAGCACTGG TGCTGAGATT AGAAGCGTCT GCACAGAGGC TGGTATGTTT GCCATCAGAG 1261 CACGGCGAAA AATTGCTACC GAGAAGGATT TCTTGGAAGC TGTAAATAAG GTCATTAAGT 1321 CTTATGCCAA ATTCAGTGCT ACTCCTCGTT ACATGACATA CAACTGCCCA ACTTTCTTGT 1381 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1441 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1501 GAAAGGACGA CAGCATATTC AAAGCTATCC CTTCACGCGT TAAGTCgaca atcaacctct 1561 ggattacaaa atttgtgaaa gatt